01978nas a2200289   4500008004100000022001400041245011000055210006900165260009500234300001200329490000600341520094400347653001401291653002101305653001601326653001101342653002301353653001801376100002501394700001601419700002601435700002901461700002601490700001701516700002601533856012901559       2007                        eng d  a1550-703300aCdS quantum dots: enzyme mediated in vitro synthesis, characterization and conjugation with plant lectins0 aCdS quantum dots enzyme mediated in vitro synthesis characteriza  a25650 NORTH LEWIS WAY, STEVENSON RANCH, CA 91381-1439 USAbAMER SCIENTIFIC PUBLISHERScDEC  a406-4130 v33 a<p>This paper presents the in vitro synthesis of CdS quantum dot nanoparticles using sulphite reductase and capping peptide in the presence of a co-factor alpha-NADPH, their characterization and conjugation with plant lectins, Jacalin and Chick pea lectin. Incubation of sulphite reductase with aqueous CdCl2, Na2SO3, and capping peptide in the presence of a-NADPH, which acts as a co-factor, resulted in the formation of CdS nanoparticles of dimensions 5-20 nm. These CdS nanoparticles were conjugated with plant lectins by EDAC mediated coupling. These observations are of significance, as it will help in the development of eco-friendly process for the formation of CdS nanoparticles and their application as fluorescent biolabels. Moreover the above studies will also help in designing a rational enzymatic strategy for the synthesis of nanomaterials of different chemical composition, shapes and sizes as well as their separation.</p>10abio-label10aCdS quantum dots10aconjugation10aEnzyme10ain vitro synthesis10aNanoparticles1 aAnsary, Abu, Ayoobul1 aKumar, Anil1 aKrishnasastry, M., V.1 aAbyaneh, Majid, Kazemian1 aKulkarni, Sulabha, K.1 aAhmad, Absar1 aKhan, Mohammad, Islam  uhttps://library.ncl.res.in/content/cds-quantum-dots-enzyme-mediated-vitro-synthesis-characterization-and-conjugation-plant-001806nas a2200301   4500008004100000022001400041245008900055210006900144260007300213300001400286490000800300520081000308653002701118653001601145653001301161653001701174653002501191653001001216653001001226100001801236700002201254700002701276700001601303700001701319700002601336700002601362856011601388       2008                        eng d  a0014-579300aTaxol and 10-deacetylbaccatinIII induce distinct changes in the dynamics of caveolae0 aTaxol and 10deacetylbaccatinIII induce distinct changes in the d  aPO BOX 211, 1000 AE AMSTERDAM, NETHERLANDSbELSEVIER SCIENCE BVcOCT  a3595-36000 v5823 a<p>Taxol treatment of HeLa cells resulted in a transient recruitment of Caveolin-1 to the cell surface followed by internalization. Interestingly, 20 min after 10-deacetylbaccatinIII (10-DAB) treatment, the caveolae displayed faster `kiss and run' dynamics while BaccatinIII (BacIII) did not induce any change. Sustained phosphorylation of Caveolin-1 is observed upon treatment and between Taxol and 10-DAB, the former shows phosphorylated Raf-1, ERK1/2 and hyperphosphorylated Bcl-2 while the later showed much less magnitude of the same. BacIII treatment did not induce phosphorylation of Raf-1 or Bcl-2. It is possible that Taxol might act on multiple targets and the side chain may be crucial. (c) 2008 Federation of European Biochemical Societies. Published by Elsevier B. V. All rights reserved.</p>10a10-DeacetylbaccatinIII10aBaccatinIII10aCaveolae10aCytotoxicity10aKiss and run dynamic10aTaxol10aTIRFM1 aAhmed, Neesar1 aDasari, Sreekanth1 aSrivastava, Saumya, S.1 aSneh, Amita1 aAhmad, Absar1 aKhan, Mohammad, Islam1 aKrishnasastry, M., V.  uhttps://library.ncl.res.in/content/taxol-and-10-deacetylbaccatiniii-induce-distinct-changes-dynamics-caveolae-0