02106nas a2200205   4500008004100000022001400041245017300055210006900228260008200297300001200379490000700391520127200398653001401670653001401684653001901698653001001717100002101727700002401748856012801772       2008                        eng d  a0042-750000aHigh frequency occurrence of single cotyledonary embryo morphotype and repetitive somatic embryogenesis in `Thompson Seedless' crossed with seven grapevine male parents0 aHigh frequency occurrence of single cotyledonary embryo morphoty  aGEILWEILERHOF, D-76833 SIEBELDINGEN, GERMANYbJKI-INSTITUT REBENZUCHTUNGcJAN  a169-1740 v473 a<p>Direct somatic embryogenesis was observed in zygotic embryos rescued from intra- and inter-specific crosses between `Thompson Seedless' and seven male parents of grapevine when the embryos were cultured on Woody Plant Medium (WPM) supplemented with benzyladenine (1 mu M). Repetitive somatic embryogenesis occurred on the same medium, which also supported a high percentage of embryo maturation, germination and plantlet development. The cultures retained embryogenic potential for more than two years. We observed a high frequency occurrence of mono-cotyledonous embryo morphotype and other morphological variations in somatic embryos of all the crosses. The percentage of embryos having mono-, di-, tri-, multiple and abnormal cotyledons varied among the crosses. The overall percentage of monocot embryos was 35.50 %, as against 38.64 % of dicot embryos, while the germination rates for mono- and dicot- embryos were 24.44 % and 24.15 %, respectively. Shoot development was poor in tri- and multiple-cotyledonary embryos, while there was no shoot formation in abnormal embryos. We assume that the relatively high occurrence of single cotyledonary morphotype, may be due to the repetitive exposure of embryogenic tissues to a medium containing benzyladenine.</p>10acotyledon10aGrapevine10asomatic embryo10aVitis1 aBharathy, P., V.1 aAgrawal, Dinesh, C.  uhttps://library.ncl.res.in/content/high-frequency-occurrence-single-cotyledonary-embryo-morphotype-and-repetitive-somatic-002839nas a2200301   4500008004100000020002200041022001400063245007100077210006900148260030100217300001200518520163500530653001602165653001402181653002202195653002102217653001902238653001002257100002002267700001802287700002102305700002402326700002402350700001902374700001902393700002302412856010202435       2008                        eng d  a978-90-6605-268-0  a0567-757200aVariability among vitis vinifera cultivars to in vitro propagation0 aVariability among vitis vinifera cultivars to in vitro propagati  aPo Box 500, 3001 Leuven 1, BelgiumbAgr & Processed Food Prod Export Dev Author; Indian Farmers Fertilizer Cooperat Ltd; Maharashtra Agro Industries Dev Corp Ltd; Indian Council Agr Res; Natl Res Ctr Grapes; Federat Indian Chambers Commerce & Ind; Natl Hort Board; Minist Food Proc IndustriecMAY  a127-1393 a<p>Response of grapevines to tissue culture has been reported to be genotype dependent, hence it becomes imperative to optimize culture conditions for newly developed varieties or cultivars, needing a large scale planting but availability of sufficient planting stock is a constraint. In the present study, conditions for in vitro propagation of six popular table grape cultivars have been standardized. Single node stem segments of cultivars 2A-Clone of Thompson Seedless, Red Globe, Crimson Seedless, Thompson Seedless, Flame Seedless and Italia cultured on ten different basal media showed varied percentages of bud break and morphogenetic responses. The percentage of bud break varied among the six cultivars. Direct rooting at basal ends of single node segments was observed in all the cultivars except in Italia. Woody plant medium induced the highest response and Eriksson medium the least in all the cultivars tested. Rooted nodal segments with shoots in axils could be established into whole plants on potting. Six cultivars showed different optimum concentrations of growth regulators for induction of maximum number of multiple shoots in both primary as well as secondary nodal segments. Shoot proliferation could be enhanced by several fold in the majority of cultivars on culture of initial shoot clumps to glass bottles instead of culture tubes. Six cultivars showed significant differences in optimum requirement of nutrients and growth regulators for shoot elongation, in vitro and ex vitro rooting and survival rate. Tissue culture plants of all the six cultivars could establish successfully in a greenhouse.</p>10abasal media10aGrapevine10agrowth regulators10amicropropagation10aTissue culture10aVitis1 aBarreto, M., S.1 aNookaraju, A.1 aJoglekar, A., M.1 aKaribasappa, G., S.1 aAgrawal, Dinesh, C.1 aAdsule, P., G.1 aSawant, I., S.1 aShikhamany, S., D.  uhttps://library.ncl.res.in/content/variability-among-vitis-vinifera-cultivars-vitro-propagation-0