01923nas a2200217   4500008004100000022001400041245009300055210006900148260007900217300001200296490000700308520112200315653001901437653002301456653002301479653001101502653002301513100002001536700002501556856012401581       2008                        eng d  a0141-022900aRapid and high biodegradation of phenols catalyzed by Candida tropicalis NCIM 3556 cells0 aRapid and high biodegradation of phenols catalyzed by Candida tr  a360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USAbELSEVIER SCIENCE INCcNOV  a431-4350 v433 a<p>Cells of 19 yeast cultures, belonging to genus Candida were screened for biodegradation of phenol. The cultures procured from NCIM culture collection and were selected so as to contain a representative of varied strains of the species. These were grown in MGYP3 medium and tested for biodegradation of phenol. C. tropicalis (CT) nos. 3118, 3556 and C. lipolytica 3472 effectively degraded &gt;90% 2 g l(-1) phenol. The C. tropicalis 3556 strain was selected for further studies as it metabolized &gt;95% phenol in just 16 h as compared to the other two which took 48 h. The maximum substrate concentration that could be completely degraded was 2 g l(-1). A study of induction, of different concentration of phenol in the growth medium showed that toxicity to cell growth increased with increasing phenol levels in growth media. Biodegradation of phenol derivatives - o-cresol, m-cresol. 2,6-dimethyl phenol, alpha-naphthol, o-chlorophenol and p-nitrophenol, by the selected three strains indicated that the specificity of each culture varied for different substrate. (C) 2008 Elsevier Inc. All rights reserved.</p>10aBiodegradation10aCandida lipolytica10aCandida tropicalis10aphenol10aPhenol derivatives1 aVarma, Rita, J.1 aGaikwad, Bhaskar, G.  uhttps://library.ncl.res.in/content/rapid-and-high-biodegradation-phenols-catalyzed-candida-tropicalis-ncim-3556-cells-001834nas a2200229   4500008004100000022001400041245009500055210006900150260006100219300001200280490000700292520101100299653001801310653001501328653002901343653001701372653002501389653002301414100002001437700002501457856012201482       2008                        eng d  a0959-399300aSpectrophotometric method for estimation of aliphatic primary amines in biological samples0 aSpectrophotometric method for estimation of aliphatic primary am  a233 SPRING STREET, NEW YORK, NY 10013 USAbSPRINGERcAPR  a573-5760 v243 a<p>A method based on Rimini test for aliphatic amines was studied and developed for quantitative estimation of aliphatic primary amines. The method involves action of the amine with acetone to form schiff base which complexes with sodium nitroprusside to give violet colour. The absorption maximum in the visible range of the spectrum, for the reaction mixture was found to be 550 nm. The pH (8-11) and reaction time scan for the assay were optimized. A linear relation of concentration (0.2-3 mg/mL) of amine against absorbance at 550 nm was established. Interference due to other reaction components was negligible (+/- 0.02 mg/mL) as compared to the sample in buffer. 1,3-dimethyl butylamine was used as the model amine and the method was applied to other amines; it was observed that when electron-withdrawing substituents are present in the molecule the reaction is retarded, as the incubation time was longer. This method is useful for estimation of aliphatic primary amine in biological samples.</p>10adetermination10aestimation10aprimary aliphatic amines10aSchiffs base10asodium nitroprusside10aspectrophotometric1 aVarma, Rita, J.1 aGaikwad, Bhaskar, G.  uhttps://library.ncl.res.in/content/spectrophotometric-method-estimation-aliphatic-primary-amines-biological-samples-0