02480nas a2200277   4500008004100000022001400041245015400055210006900209260008900278300001200367490000700379520142000386653001401806653001301820653002001833653003501853653002301888653001801911100002501929700002501954700002001979700002401999700002802023700002102051856013002072       2006                        eng d  a0885-576500aDifferentially expressed gene transcripts in roots of resistant and susceptible chickpea plant (Cicer arietinum L.) upon Fusarium oxysporum infection0 aDifferentially expressed gene transcripts in roots of resistant   a24-28 OVAL RD, LONDON NW1 7DX, ENGLANDbACADEMIC PRESS LTD ELSEVIER SCIENCE LTDcAPR  a176-1880 v683 a<p>Differentially expressed genes in chickpea, (Cicer arietinum L.) during root infection by Fusarium oxysportan f sp. ciceri Racel, were identified using cDNA-RAPD and cDNA-AFLP approaches. The former employed decamer primers on cDNA template and revealed nine differentially expressed transcripts in the resistant-infected chickpea variety. Among the 2000 transcript-derived fragments (TDFs) screened by cDNA-AFLP, 273 were differentially expressed in chickpea roots during Fusaritan infection. Only 13.65% of the TDFs were differentially regulated during pathogen challenge, while the other 86% were expressed non-differentially during the process of pathogen infection in chickpea roots. Nineteen TDFs, which expressed differentially in the resistant-infected chickpea variety were cloned and sequenced. Two of these TDFs were similar to transcription factors like WRKY proteins and 14-3-3 proteins, while three TDFs represented the NBS-LRR-type gene sequences. Two TDFs had sequence identity to genes known to have function in defense. The RAPID TDF CaFRi60 showed sequence identity to gamma-glutamyl-cysteine synthetase. Among the TDFs examined by cDNA-AFLP, 19 were confirmed by Reverse Northern blot to be differentially expressed. The data confirms the effectiveness of the cDNA-AFLP technique in detecting differentially expressed genes during pathogenesis. (c) 2006 Elsevier Ltd. All rights reserved.</p>10acDNA-AFLP10aChickpea10aCicer arietinum10adifferentially expressed genes10afusarium oxysporum10aTranscriptome1 aNimbalkar, Suhas, B.1 aHarsulkar, Abhay, M.1 aGiri, Ashok, P.1 aSainani, Mohini, N.1 aFranceschi, Vincent, R.1 aGupta, Vidya, S.  uhttps://library.ncl.res.in/content/differentially-expressed-gene-transcripts-roots-resistant-and-susceptible-chickpea-plant-002302nas a2200265   4500008004100000022001400041245007900055210006900134260007300203300001000276490000800286520133100294653002501625653003001650653003701680653003501717653003201752100002301784700002001807700002401827700002101851700002301872700002801895856011301923       2009                        eng d  a0378-111900aWinged bean chymotrypsin inhibitors retard growth of Helicoverpla anmigera0 aWinged bean chymotrypsin inhibitors retard growth of Helicoverpl  aPO BOX 211, 1000 AE AMSTERDAM, NETHERLANDSbELSEVIER SCIENCE BVcFEB  a80-850 v4313 a<p>Two putative Kunitz-type chymotrypsin inhibitor genes (WC12 and WC15) were isolated from winged bean (Psophocarpus tetragonolobus (L.) DC). While WC12 has previously been characterized, WC15 represents a new member of the WC1 family. WC15 was exclusively expressed in winged bean seeds. Theoretical translation of both the genes resulted into polypeptides of 207 amino acids with 86% sequence similarity. The polypeptide sequences contain four half-cysteine residues and a well-conserved Leu(65)-Ser(66) reactive site, typical for chymotrypsin inhibitors. WC15 and WC12 were expressed in Pichia pastoris and the recombinant proteins were assayed against various proteinases. Both the inhibitors strongly inhibited commercially available bovine chymotrypsin. More importantly, gut proteinases of Helicoverpa armigera larvae that damage many important crop plants, were inhibited by WC12 and WC15. In addition, both proteinase inhibitors demonstrated significant reduction of growth of H. armigera larvae after feeding on inhibitor incorporated artificial diets. The inhibitory effects of WC12 and WC15 on activity of proteinases and larval growth make these proteins and their genes promising candidates for enhancing plant defense against H. armigera using transgenic plants. (C) 2008 Elsevier B.V. All rights reserved.</p>10aAntimetabolic effect10aDigestive gut proteinases10aKunitz-type proteinase inhibitor10aOrgan-specific mRNA expression10aPsophocarpus tetragonolobus1 aTelang, Manasi, A.1 aGiri, Ashok, P.1 aPyati, Prashant, S.1 aGupta, Vidya, S.1 aTegeder, Mechthild1 aFranceschi, Vincent, R.  uhttps://library.ncl.res.in/content/winged-bean-chymotrypsin-inhibitors-retard-growth-helicoverpla-anmigera-0