02172nas a2200157   4500008004100000022001400041245012100055210006900176260014500245300001200390490000700402520144200409100001301851700002001864856013001884       2009                        eng d  a1344-630400aEvaluation of high performance liquid chromatography purified leptospiral antigen for the diagnosis of leptospirosis0 aEvaluation of high performance liquid chromatography purified le  aJPN J INFECT DIS ED OFF NATL INST INFECTIOUS DISEASES TOYAMA 1-23-1, SHINJUKU-KU, TOKYO, 162-8640, JAPANbNATL INST INFECTIOUS DISEASEScNOV  a428-4310 v623 a<p>Leptospirosis is a re-emerging infection, and often occurs in outbreaks. Early diagnosis is important for appropriate control measures and treatment. The sensitivity and specificity of the diagnostic test depends on the antigen, which is used for the detection of antibodies. In the present study, an effort was made to purify and characterize leptospiral antigens, and their performance in the laboratory was assessed. Leptospira biflexa semaranga, Patoc was subjected to processing by the heat extraction method, and the crude antigenic preparation was subjected to high-performance liquid chromatography for purification. The purified antigen fractions were subjected to enzyme-linked immunosorbent assay (ELISA) to determine their reactivity. Only the reactive fractions were tested for specificity by ELISA by allowing the fractions to react with positive sera of patients with non-leptospiral illness. H1, the purified antigen fraction of the heat-extracted preparation, was found to be the most reactive and specific of all fractions tested. The protein was found to have a molecular weight of 50 kDa. The performance of H1 in the laboratory was assessed by formulating an in-house ELISA using the H1 antigen. The results were compared with those of microscopic agglutination testing and commercial ELISA. The specificity and sensitivity of in-house ELISA using the H1 antigen were found to be 93.3 and 85.0%, respectively.</p>1 aPol, Sae1 aBharadwaj, Renu  uhttps://library.ncl.res.in/content/evaluation-high-performance-liquid-chromatography-purified-leptospiral-antigen-diagnosis-0