01909nas a2200217   4500008004100000022001400041245016500055210006900220260007900289300001200368490000800380520103200388653001601420653002401436653001001460653002101470653003101491100002201522700002101544856012601565       2015                        eng d  a0273-228900aPossible correlation between bile salt hydrolysis and ahl deamidation: staphylococcus epidermidis RM1, a potent quorum quencher and bile salt hydrolase producer0 aPossible correlation between bile salt hydrolysis and ahl deamid  a999 RIVERVIEW DRIVE SUITE 208, TOTOWA, NJ 07512 USAbHUMANA PRESS INCcMAY  a140-1500 v1763 a<p>The aim of the present work was to isolate a bile salt hydrolase (BSH) producer from fermented soy curd and explore the ability of the BSH produced to cleave bacterial quorum sensing signals. Bacterial isolates with possible ability to deconjugate bile salts were enriched and isolated on De Man, Rogosa and Sharpe (MRS) medium containing 0.2 % bile salts. BSH-producing positive isolate with orange-pink-pigmented colonies was isolated and was identified as a strain of Staphylococcus epidermidis using biochemical and phylogenetic tools. S. epidermidis RM1 was shown to possess both potent BSH and N-acyl homoserine lactone (AHL) cleavage activity. Genetic basis of this dual-enzyme activity was explored by means of specific primers designed using S. epidermidis ATCC 12228 genome as template. It was observed that a single enzyme was not responsible for both the activity. Two different genetic elements corresponding to each of the enzymatic activity were successfully amplified from the genomic DNA of the isolate.</p>10aAHL acylase10aBile salt hydrolase10aCV02610aQuorum quenching10aStaphylococcus epidermidis1 aMukherji, Ruchira1 aPrabhune, Asmita  uhttps://library.ncl.res.in/content/possible-correlation-between-bile-salt-hydrolysis-and-ahl-deamidation-staphylococcus-0