01968nas a2200229   4500008004100000022001400041245019500055210006900250260007900319300001400398490000800412520102600420653002001446653001001466653002101476653003001497653001201527100002201539700002601561700002101587856013001608       2013                        eng d  a0273-228900aCrystalline xylitol production by a novel yeast, pichia caribbica (HQ222812), and its application for quorum sensing inhibition in gram-negative marker strain chromobacterium violaceum CV0260 aCrystalline xylitol production by a novel yeast pichia caribbica  a999 RIVERVIEW DRIVE SUITE 208, TOTOWA, NJ 07512 USAbHUMANA PRESS INCcMAR  a1753-17630 v1693 a<p>Xylitol, a sugar alcohol, is fast gaining ground over other artificial sugar substitutes owing to its advantageous properties. Xylitol is a safer alternative for diabetics because of insulin-independent metabolism. It has beneficial properties suitable to form an important part of odontological formulations. Conventional commercial production of xylitol involves harsh chemical method operating at high temperature and pressure. Thus, microbial production of xylitol is preferred over chemical method, and yeasts have been extensively exploited for this purpose. In the present manuscript, quantitative production of xylitol from d-xylose with the yield of 0.852 gm/gm and volumetric productivity of 1.83 gm/l/h in crystalline form, using novel yeast Pichia caribbica is reported. Also, a mild, safe procedure for product extraction is described. The ability of xylitol to act as a quorum sensing antagonist in gram-negative marker strain Chromobacterium violaceum CV026 has been demonstrated for the first time.</p>10aCrystallization10aCV02610aPichia caribbica10aQuorum sensing antagonist10axylitol1 aMukherji, Ruchira1 aJoshi-Navare, Kasturi1 aPrabhune, Asmita  uhttps://library.ncl.res.in/content/crystalline-xylitol-production-novel-yeast-pichia-caribbica-hq222812-and-its-application-001819nas a2200229   4500008004100000022001400041245011000055210006900165260011000234300001200344490000700356520091600363100002601279700002201305700002601327700002001353700001901373700002101392700002101413700002501434856013001459       2013                        eng d  a1463-926200aFluorescent sophorolipid molecular assembly and its magnetic nanoparticle loading: a pulsed laser process0 aFluorescent sophorolipid molecular assembly and its magnetic nan  aTHOMAS GRAHAM HOUSE, SCIENCE PARK, MILTON RD, CAMBRIDGE CB4 0WF, CAMBS, ENGLANDbROYAL SOC CHEMISTRYcJAN  a943-9530 v153 a<p>We report realization of strong green fluorescence in a fully biocompatible highly spherical mesoscale molecular assembly of sophorolipid created by pulsed UV laser processing of a water-based dispersion of sophorolipid. Remarkably, the said fluorescence is totally absent in the original molecule of sophorolipid and is thus the property of the pulsed laser induced modifications in the molecule and the assembly. We have separately examined the consequences of laser irradiation for glucose and oleic acid components which form the sophorolipid. This fluorescence character appears to be driven by the oleic acid component while the assembly process is assisted by the glucose component. Importantly the laser synthesized mesostructures can be easily redispersed in an aqueous medium after being dried and can also be loaded with magnetic nanoparticles (magnetite) for inducing the hyperthermia effect.</p>1 aSingh, Pradeep, Kumar1 aMukherji, Ruchira1 aJoshi-Navare, Kasturi1 aBanerjee, Abhik1 aGokhale, Rohan1 aNagane, Satyawan1 aPrabhune, Asmita1 aOgale, Satishchandra  uhttps://library.ncl.res.in/content/fluorescent-sophorolipid-molecular-assembly-and-its-magnetic-nanoparticle-loading-pulsed-001599nas a2200253   4500008004100000022001400041245014700055210006900202260007100271300001600342490000600358520064800364653002001012653001401032653002501046653001901071100002201090700002001112700002701132700002101159700002101180700001801201856012601219       2013                        eng d  a1944-824400aSelective imaging of quorum sensing receptors in bacteria using fluorescent Au nanocluster probes surface functionalized with signal molecules0 aSelective imaging of quorum sensing receptors in bacteria using   a1155 16TH ST, NW, WASHINGTON, DC 20036 USAbAMER CHEMICAL SOCcDEC  a13076-130810 v53 a<p>Fluorescent ultrasmall gold clusters decorated with bacterial quorum sensing signal molecules, acyl homoserine lactone, are synthesized. These fluorescent probes are found to have emission in the near-infrared spectral region advantageous for bioimaging. Imaging studies using different. strains of bacteria with and without acyl homoserine lactone receptors with the aid of confocal microscopy have shown that the probe interacts preferentially with cells possessing these receptors. This indicates that, with appropriate surface functionalization, the Au clusters can be used for receptor specific detection with enhanced selectivity.</p>10aAu nanoclusters10aBiosensor10afluorescence imaging10aquorum sensing1 aMukherji, Ruchira1 aSamanta, Anupam1 aIllathvalappil, Rajith1 aChowdhury, Somak1 aPrabhune, Asmita1 aDevi, Nandini  uhttps://library.ncl.res.in/content/selective-imaging-quorum-sensing-receptors-bacteria-using-fluorescent-au-nanocluster-002532nas a2200253   4500008004100000022001400041245008300055210006900138260007300207300000900280490000700289520165900296653001801955653002201973653002601995653002702021100002402048700002202072700001502094700001302109700002102122700002302143856011202166       2014                        eng d  a0928-493100aControlled release of antimicrobial Cephalexin drug from silica microparticles0 aControlled release of antimicrobial Cephalexin drug from silica   aPO BOX 211, 1000 AE AMSTERDAM, NETHERLANDSbELSEVIER SCIENCE BVcJAN  a9-140 v343 a<p>Release of antimicrobial drugs in a controlled fashion for extended duration of time has been investigated for long. Such controlled-drug-releasing materials show promising applications in medicinal bandages. Along with antimicrobial agents, one could also incorporate other therapeutic drugs, to make such bandages more versatile. In this context, silica micro particles were synthesized using direct reduction method, in which the synthesis was done in the presence of Cephalexin. Cephalexin was chosen as an antimicrobial candidate. The morphological characterization shows formation of monodispersed, silica microparticles of similar to 200 nm in size. The FTIR spectroscopy shows weak interaction of the drug molecule at its hydroxide (OH) site with oxygen ions on the silica surface. Upon conjugation, the UV-vis spectroscopy shows persistence of the Cephalexin signature, especially its R group, confirming its antimicrobial activity even after conjugation. Loading studies reveal 12% Cephalexin loading on silica. The antimicrobial studies were done on three micro-organisms, namely, Staphylococcus aureus, Bacillus subtilis and Escherichia coli. Using zone-of-inhibition studies, it was found that E. coli, did not respond to the delivery of Cephalexin either directly or via microparticles. However, for both S. aureus and B. subtilis, the particles showed controlled release of Cephalexin for the duration of 48 h and continued maintenance and even increase in the zone of inhibition. This work demonstrates an effective protocol to prepare antimicrobial patches for controlled drug delivery. (C) 2013 Elsevier B.V. All rights reserved.</p>10aAntimicrobial10aMedicinal bandage10aSilica microparticles10asustained drug release1 aRao, B., V. Bhaskar1 aMukherji, Ruchira1 aShitre, G.1 aAlam, F.1 aPrabhune, Asmita1 aKale, Sangeeta, N.  uhttps://library.ncl.res.in/content/controlled-release-antimicrobial-cephalexin-drug-silica-microparticles-002274nas a2200241   4500008004100000022001400041245014400055210006900199260007900268300000800347490000700355520135400362653002901716653001001745653001001755653002101765100002201786700002901808700002601837700001901863700002101882856012901903       2014                        eng d  a0141-022900aNew role for penicillin acylases: degradation of acyl homoserine lactone quorum sensing signals by Kluyvera citrophila penicillin G acylase0 aNew role for penicillin acylases degradation of acyl homoserine   a360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USAbELSEVIER SCIENCE INCcMAR  a1-70 v563 a<p>Use of penicillin acylases for the production of semi-synthetic penicillins is well-known. Escherichia coli penicillin G acylase (EcPGA) has been extensively used for this purpose; however, Kluyvera citrophila penicillin G acylase (KcPGA) is assumed to be a better substitute, owing to its increased resilience to extreme pH conditions and ease of immobilization. In the present article we report a new dimension for the amidase activity of KcPGA by demonstrating its ability to cleave bacterial quorum sensing signal molecules, acyl homoserine lactones (AHL) with acyl chain length of 6-8 with or without oxo-substitution at third carbon position. Initial evidence of AHL degrading capability of KcPGA was obtained using CV026 based bioassay method. Kinetic studies performed at pH 8.0 and 50 degrees C revealed 3-oxo-C6 HSL to be the best substrate for the enzyme with V-max and K-m values of 21.37+0.85 mM/h/mg of protein and 0.1+0.01 mM, respectively. C6 HSL was found to be the second best substrate with V-max. and K-m value of 10.06+0.27 mM/h/mg of protein and 0.28+0.02 mM, respectively. Molecular modeling and docking studies performed on the active site of the enzyme support these findings by showing the fitting of AHLs perfectly within the hydrophobic pocket of the enzyme active site. (c) 2013 Elsevier Inc. All rights reserved.</p>10aAcyl homoserine lactones10aCV02610aKcPGA10aQuorum quenching1 aMukherji, Ruchira1 aVarshney, Nishant, Kumar1 aPanigrahi, Priyabrata1 aSuresh, C., G.1 aPrabhune, Asmita  uhttps://library.ncl.res.in/content/new-role-penicillin-acylases-degradation-acyl-homoserine-lactone-quorum-sensing-signals-002059nas a2200145   4500008004100000022001400041245013800055210006900193260009700262300001100359520137300370100002201743700002101765856012701786       2014                        eng d  a1537-744X00aNovel glycolipids synthesized using plant essential oils and their application in quorum sensing inhibition and as antibiofilm agents0 aNovel glycolipids synthesized using plant essential oils and the  a410 PARK AVENUE, 15TH FLOOR, \#287 PMB, NEW YORK, NY 10022 USAbHINDAWI PUBLISHING CORPcJAN  a8907093 a<p>Essential oils (EOs) form an important part of traditional medicine so their anti-microbial and, in the recent past, antiquorum sensing activity has been well studied. However it is likely that due to their hydrophobic nature and reduced solubility in aqueous environments full potential of their activity cannot be realized. hence it is only rational to formulate a process to make these molecules more polar in nature. The present paper reports synthesis of sophorolipids using 12 different essential oils as substrates, thus providing surfactant-like properties to these EOs. The synthesis protocol makes the use of Candida bombicola ATCC 22214 as producer organism. The production process required 7 days of incubation at 28 degrees C and 180 rpm. Preliminary characterization of the synthesized essential oil sophorolipids (EOSLs) was performed using thin layer chromatography (TLC) and Fourier transform infrared spectroscopy (FTIR). Additionally, essential oils that were incapable of mediating quorum sensing inhibition (QSI) on their own became potent quorum sensing inhibitors upon conversion into their corresponding EOSLs. Antibiofilm potential of these EOSLs was also demonstrated using V. cholerae as test organism. Use of essential oils as substrates for glycolipid synthesis has not been attempted previously, and hence this is the first report.</p>1 aMukherji, Ruchira1 aPrabhune, Asmita  uhttps://library.ncl.res.in/content/novel-glycolipids-synthesized-using-plant-essential-oils-and-their-application-quorum-002159nas a2200217   4500008004100000022001400041245012100055210006900176260005700245300001200302490000700314520133900321653002001660653002301680653001301703653002701716653003001743100002201773700002101795856012501816       2015                        eng d  a0959-399300aNew class of bacterial quorum sensing antagonists: glycomonoterpenols synthesized using linalool and alpha terpineol0 aNew class of bacterial quorum sensing antagonists glycomonoterpe  a233 SPRING ST, NEW YORK, NY 10013 USAbSPRINGERcJUN  a841-8490 v313 a<p>With increasing burden of antibiotic resistant microorganism search for newer drug targets and potent drug molecules is a never ending scenario. Quorum sensing (QS), the phenomenon of bacterial cross-talk, is one such target that has captured the attention of many and has been touted as the future of new age antimicrobials. Quorum sensing has the potential to regulate a plethora of bacterial virulence phenotypes and search of molecules with powerful quorum sensing inhibitory (QSI) capacity are underway. Monoterpene alcohols like linalool and alpha terpineol have been shown to possess antimicrobial and anti-biofilm activity. However in this article we attempt to bring forth a new class of compounds, glycomonoterpenols, derived from monoterpenoids alcohols. These glycomonoterpenols have been synthesized using Candida bombicola ATCC 22214 by feeding the cells with linalool and alpha terpineol respectively as substrates in 10 % glucose, production medium. The advantage of these molecules over their parent compound is their additional surfactant like property, increased solubility and enhanced QSI potential. A variety of gram-negative bacteria capable of elaborating quorum sensing mediated phenotypes have been selected and both these glycoterpenoid derivatives have been shown to possess strong anti-QS activity.</p>10aAlpha-terpineol10aGlycomonoterpenols10aLinalool10aPseudomonas aeruginosa10aQuorum sensing inhibition1 aMukherji, Ruchira1 aPrabhune, Asmita  uhttps://library.ncl.res.in/content/new-class-bacterial-quorum-sensing-antagonists-glycomonoterpenols-synthesized-using-001909nas a2200217   4500008004100000022001400041245016500055210006900220260007900289300001200368490000800380520103200388653001601420653002401436653001001460653002101470653003101491100002201522700002101544856012601565       2015                        eng d  a0273-228900aPossible correlation between bile salt hydrolysis and ahl deamidation: staphylococcus epidermidis RM1, a potent quorum quencher and bile salt hydrolase producer0 aPossible correlation between bile salt hydrolysis and ahl deamid  a999 RIVERVIEW DRIVE SUITE 208, TOTOWA, NJ 07512 USAbHUMANA PRESS INCcMAY  a140-1500 v1763 a<p>The aim of the present work was to isolate a bile salt hydrolase (BSH) producer from fermented soy curd and explore the ability of the BSH produced to cleave bacterial quorum sensing signals. Bacterial isolates with possible ability to deconjugate bile salts were enriched and isolated on De Man, Rogosa and Sharpe (MRS) medium containing 0.2 % bile salts. BSH-producing positive isolate with orange-pink-pigmented colonies was isolated and was identified as a strain of Staphylococcus epidermidis using biochemical and phylogenetic tools. S. epidermidis RM1 was shown to possess both potent BSH and N-acyl homoserine lactone (AHL) cleavage activity. Genetic basis of this dual-enzyme activity was explored by means of specific primers designed using S. epidermidis ATCC 12228 genome as template. It was observed that a single enzyme was not responsible for both the activity. Two different genetic elements corresponding to each of the enzymatic activity were successfully amplified from the genomic DNA of the isolate.</p>10aAHL acylase10aBile salt hydrolase10aCV02610aQuorum quenching10aStaphylococcus epidermidis1 aMukherji, Ruchira1 aPrabhune, Asmita  uhttps://library.ncl.res.in/content/possible-correlation-between-bile-salt-hydrolysis-and-ahl-deamidation-staphylococcus-002736nas a2200277   4500008004100000022001400041245013100055210006900186260008000255300001400335490000700349520172600356653001702082653002802099653002302127653002102150653001402171653002302185653002002208100002602228700001802254700002202272700002602294700001902320856011902339       2015                        eng d  a1367-543500aSequence and structure-based comparative analysis to assess, identify and improve the thermostability of penicillin G acylases0 aSequence and structurebased comparative analysis to assess ident  aTIERGARTENSTRASSE 17, D-69121 HEIDELBERG, GERMANYbSPRINGER HEIDELBERGcNOV  a1493-15060 v423 a<p>Penicillin acylases are enzymes employed by the pharmaceutical industry for the manufacture of semi-synthetic penicillins. There is a continuous demand for thermostable and alkalophilic enzymes in such applications. We have carried out a computational analysis of known penicillin G acylases (PGAs) in terms of their thermostable nature using various protein-stabilizing factors. While the presence of disulfide bridges was considered initially to screen putative thermostable PGAs from the database, various other factors such as high arginine to lysine ratio, less content of thermolabile amino acids, presence of proline in beta-turns, more number of ion-pair and other non-bonded interactions were also considered for comparison. A modified consensus approach designed could further identify stabilizing residue positions by site-specific comparison between mesostable and thermostable PGAs. A most likely thermostable enzyme identified from the analysis was PGA from Paracoccus denitrificans (PdPGA). This was cloned, expressed and tested for its thermostable nature using biochemical and biophysical experiments. The consensus site-specific sequence-based approach predicted PdPGA to be more thermostable than Escherichia coli PGA, but not as thermostable as the PGA from Achromobacter xylosoxidans. Experimental data showed that PdPGA was comparatively less thermostable than Achromobacter xylosoxidans PGA, although thermostability factors favored a much higher stability. Despite being mesostable, PdPGA being active and stable at alkaline pH is an advantage. Finally, several residue positions could be identified in PdPGA, which upon mutation selectively could improve the thermostability of the enzyme.</p>10aAlkalistable10abeta-lactam antibiotics10aConsensus approach10aDisulfide bridge10aIon pairs10aPenicillin acylase10aThermostability1 aPanigrahi, Priyabrata1 aChand, Deepak1 aMukherji, Ruchira1 aRamasamy, Sureshkumar1 aSuresh, C., G.  uhttps://library.ncl.res.in/content/sequence-and-structure-based-comparative-analysis-assess-identify-and-improve-001813nas a2200193   4500008004100000022001400041245008400055210006900139260007400208300001000282490000700292520109600299100002301395700001801418700002201436700002101458700002501479856011501504       2016                        eng d  a0015-563200aBiological activity of sophorolipids and their possible use as antiviral agents0 aBiological activity of sophorolipids and their possible use as a  aVAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDSbSPRINGERcJAN  a85-890 v613 a<p>Sophorolipids are surface active glycolipids consisting of a hydrophilic sophorose unit and a hydrophobic portion composed of a fatty acid tail. Crude sophorolipid sample contains both acidic and lactonic forms of sophorolipid with different degrees of acetylation and varying lengths of the fatty acid chains depending on the substrates used in the production process. Carboxylic end in the acidic form of the fatty acid is free, whereas in the lactonic form, it is internally esterified. Sophorolipids show different physicochemical properties with wide range of applications for each structural compound. Lactonic form of sophorolipids shows surface tension reducing ability and biological activity, whereas the acidic form possesses better foam forming ability and higher solubility. Presence of acetyl groups gives hydrophilic nature to the sophorolipids which promotes its antiviral and cytokine-stimulating properties. The aim of this review is to explore and suggest the plausibility of sophorolipids as therapeutic and prophylactic agents for the treatment of viral diseases.</p>1 aBorsanyiova, Maria1 aPatil, Amrita1 aMukherji, Ruchira1 aPrabhune, Asmita1 aBopegamage, Shubhada  uhttps://library.ncl.res.in/content/biological-activity-sophorolipids-and-their-possible-use-antiviral-agents-002026nas a2200169   4500008004100000245009900041210006900140260000800209300001400217490000900231520140500240100001801645700002501663700002201688700002101710856012501731       2017                        eng d00aBiosynthesis of glycomonoterpenes to attenuate quorum sensing associated virulence in bacteria0 aBiosynthesis of glycomonoterpenes to attenuate quorum sensing as  cAPR  a1533-15480 v181 3 aThe acquisition of multidrug resistance in bacteria has become a bigger threat of late, mainly due to the bacterial signaling phenomenon, quorum sensing (QS). QS, among a population of bacteria, initiates the formation of biofilms and offers myriad advantages to bacteria. Burgeoning antibiotic resistance in biofilm-producing bacteria has motivated efforts toward finding new alternatives to these traditional antimicrobials. In the present study, we report the increased solubility and additional quorum quenching as well as biofilm disruption activity of glyco-derivatives of monoterpenes (citral and citronellal). Glycomonoterpenes of citral and citronellal were synthesized via conjugation of the monoterpenes with glucose by the non-pathogenic yeast Candida bombicola (ATCC 22214). Structural elucidation of newly synthesized glycomonoterpenes showed that one synthesized using citronellal contains three major lactonic forms with molecular weight 492.43, 473.47, and 330.39 Da whereas the one produced using citral has an acidic form with molecular weight 389.33 and 346.23 Da. The glycomonoterpenes were able to individually inhibit QS, mediated through various medium-chain and long-chain N-acyl homoserine lactones (AHLs). These new compounds are interesting additions to the known range of quorum sensing inhibitors (QSIs) and could be further explored for potential clinical applications.1 aPatil, Amrita1 aJoshi-Navre, Kasturi1 aMukherji, Ruchira1 aPrabhune, Asmita  uhttps://library.ncl.res.in/content/biosynthesis-glycomonoterpenes-attenuate-quorum-sensing-associated-virulence-bacteria