01813nas a2200193   4500008004100000022001400041245008400055210006900139260007400208300001000282490000700292520109600299100002301395700001801418700002201436700002101458700002501479856011501504       2016                        eng d  a0015-563200aBiological activity of sophorolipids and their possible use as antiviral agents0 aBiological activity of sophorolipids and their possible use as a  aVAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDSbSPRINGERcJAN  a85-890 v613 a<p>Sophorolipids are surface active glycolipids consisting of a hydrophilic sophorose unit and a hydrophobic portion composed of a fatty acid tail. Crude sophorolipid sample contains both acidic and lactonic forms of sophorolipid with different degrees of acetylation and varying lengths of the fatty acid chains depending on the substrates used in the production process. Carboxylic end in the acidic form of the fatty acid is free, whereas in the lactonic form, it is internally esterified. Sophorolipids show different physicochemical properties with wide range of applications for each structural compound. Lactonic form of sophorolipids shows surface tension reducing ability and biological activity, whereas the acidic form possesses better foam forming ability and higher solubility. Presence of acetyl groups gives hydrophilic nature to the sophorolipids which promotes its antiviral and cytokine-stimulating properties. The aim of this review is to explore and suggest the plausibility of sophorolipids as therapeutic and prophylactic agents for the treatment of viral diseases.</p>1 aBorsanyiova, Maria1 aPatil, Amrita1 aMukherji, Ruchira1 aPrabhune, Asmita1 aBopegamage, Shubhada  uhttps://library.ncl.res.in/content/biological-activity-sophorolipids-and-their-possible-use-antiviral-agents-002008nas a2200169   4500008004100000245009300041210006900134260000800203300001400211490000800225520140700233100002001640700001801660700002101678700001701699856012201716       2016                        eng d00aInhibition of quorum-sensing-mediated biofilm formation in Cronobacter sakazakii strains0 aInhibition of quorumsensingmediated biofilm formation in Cronoba  cSEP  a1708-17140 v1623 aThe present study investigated plant extracts for their anti-quorum-sensing (QS) potential to inhibit the biofilm formation in Cronobacter sakazakii strains. The bioassay based on loss of pigment production by Chromobacterium violaceum 026 and Agrobacterium tumefaciens NTL4 (pZLR4) was used for initial screening of the extracts. Further, the effect of extracts on the inhibition of QS-mediated biofilm in C. sakazakii isolates was evaluated using standard crystal violet assay. The effect on biofilm texture was studied using SYTO9 staining and light and scanning electron microscopy. Among the tested extracts, Piper nigrum and Cinnamomum verum at 100 ppm resulted in 78 and 68% reduction in the production of violacein as well as blue-green colour in both biosensor strains. A higher inhibitory activity (>50 %) on biofilm formation in C. sakazakii was observed for Pip. nigrum and Cin. verum, whereas the other extracts possessed moderate (25-50 %) and minimal (<25 %) inhibitory activities. Further, the fluorescent and scanning electron microscopic images indicated a major disruption in the architecture of biofilms of tested strains by Pip. nigrum. This study points to the possibility of using Pip. nigrum and Cin. verum as inhibitor of QS-mediated biofilm formation by C. sakazakii that could be further explored for novel bioactive molecules to limit the emerging infections of C. sakazakii1 aSingh, Niharika1 aPatil, Amrita1 aPrabhune, Asmita1 aGoel, Gunjan  uhttps://library.ncl.res.in/content/inhibition-quorum-sensing-mediated-biofilm-formation-cronobacter-sakazakii-strains02026nas a2200169   4500008004100000245009900041210006900140260000800209300001400217490000900231520140500240100001801645700002501663700002201688700002101710856012501731       2017                        eng d00aBiosynthesis of glycomonoterpenes to attenuate quorum sensing associated virulence in bacteria0 aBiosynthesis of glycomonoterpenes to attenuate quorum sensing as  cAPR  a1533-15480 v181 3 aThe acquisition of multidrug resistance in bacteria has become a bigger threat of late, mainly due to the bacterial signaling phenomenon, quorum sensing (QS). QS, among a population of bacteria, initiates the formation of biofilms and offers myriad advantages to bacteria. Burgeoning antibiotic resistance in biofilm-producing bacteria has motivated efforts toward finding new alternatives to these traditional antimicrobials. In the present study, we report the increased solubility and additional quorum quenching as well as biofilm disruption activity of glyco-derivatives of monoterpenes (citral and citronellal). Glycomonoterpenes of citral and citronellal were synthesized via conjugation of the monoterpenes with glucose by the non-pathogenic yeast Candida bombicola (ATCC 22214). Structural elucidation of newly synthesized glycomonoterpenes showed that one synthesized using citronellal contains three major lactonic forms with molecular weight 492.43, 473.47, and 330.39 Da whereas the one produced using citral has an acidic form with molecular weight 389.33 and 346.23 Da. The glycomonoterpenes were able to individually inhibit QS, mediated through various medium-chain and long-chain N-acyl homoserine lactones (AHLs). These new compounds are interesting additions to the known range of quorum sensing inhibitors (QSIs) and could be further explored for potential clinical applications.1 aPatil, Amrita1 aJoshi-Navre, Kasturi1 aMukherji, Ruchira1 aPrabhune, Asmita  uhttps://library.ncl.res.in/content/biosynthesis-glycomonoterpenes-attenuate-quorum-sensing-associated-virulence-bacteria01469nas a2200181   4500008004100000245010400041210006900145260000800214300001200222490000600234520081300240100002001053700001801073700003101091700001801122700001701140856013001157       2017                        eng d00aDiverse profiles of N-acyl-homoserine lactones in biofilm forming isolates of Cronobacter sakazakii0 aDiverse profiles of Nacylhomoserine lactones in biofilm forming   cOCT  a275-2810 v83 a<p>The present study investigates the role of quorum sensing (QS) molecules expressed by C. sakazakii in biofilm formation and extracellular polysaccharide expression. The QS signaling was detected using Chromobacterium violaceum 026 and Agrobacterium tumefaciens NTL4(pZLR4) based bioassay. Long chain N-acyl-homoserine lactones (AHLs) with C6- C18 chain length were identified using High Performance Liquid Chromatography and Liquid Chromatography-High Resolution Mass Spectrometry. A higher Specific Biofilm Formation (SBF) index (p &lt; 0.05) with the presence of genes associated with cellulose biosynthesis (bcsA, bcsC and bcsG) was observed in the strains. AHLs and their mechanisms can serve as novel targets for developing technologies to eradicate and prevent biofilm formation by C. sakazakii.</p>1 aSingh, Niharika1 aPatil, Amrita1 aPrabhune, Asmita, Ashutosh1 aRaghav, Mamta1 aGoel, Gunjan  uhttps://library.ncl.res.in/content/diverse-profiles-n-acyl-homoserine-lactones-biofilm-forming-isolates-cronobacter-sakazakii