02397nas a2200241   4500008004100000022001400041245012000055210006900175260010900244300001200353490000800365520145200373653002301825653001801848653002901866100002001895700001701915700002401932700002801956700002401984700002002008856012702028       2010                        eng d  a0378-874100aCytotoxic activity of 9,10-dihydro-2,5-dimethoxyphenanthrene-1,7-diol from Eulophia nuda against human cancer cells0 aCytotoxic activity of 910dihydro25dimethoxyphenanthrene17diol fr  aELSEVIER HOUSE, BROOKVALE PLAZA, EAST PARK SHANNON, CO, CLARE, 00000, IRELANDbELSEVIER IRELAND LTDcMAR  a251-2530 v1283 a<p>Ethnopharmacological relevance: Eulophia nuda L. (Orchidaceae) is a medicinally important terrestrial orchid used for the treatment of tumours and various health problems by the local healers throughout the Western Ghats region in Maharashtra (India). Aim of the study: To isolate the active molecule from Eulophia nuda and to study its cytotoxic potential against human cancer cells. Materials and methods: The crude methanolic extract of Eulophia nuda tubers was fractionated by stepwise gradient of the solvents chloroform-methanol to isolate the pure compound. Isolated pure compound was assessed for its cytotoxic potential against human breast cancer cell lines, MCF-7 and MDA-MB-231 using MTT assay. Structure elucidation of the isolated active compound was carried out by extensive spectroscopic analysis including (1)H NMR, (13)C NMR, NOESY, COSY, LC-MS and IR. Results: The isolated active molecule was identified as phenanthrene derivative 9,10-dihydro-2,5-dimethoxyphenanthrene-1,7-diol. This compound showed good antiproliferative activity against human breast cancer cell lines MCF-7 (91%) and MDA-MB-231 (85%) at 1000 mu g/ml concentration. Conclusion: 9,10-Dihydro-2,5-dimethoxyphenanthrene-1,7-diol from Eulophia nuda tubers showed good growth suppressive effect against human cancer cell lines MCF-7 and MDA-MB-231 making it a potential biomolecule against human cancer. (C) 2009 Elsevier Ireland Ltd. All rights reserved.</p>10aCytotoxic activity10aEulophia nuda10aPhenanthrene derivatives1 aShriram, Varsha1 aKumar, Vinay1 aKishor, P., B. Kavi1 aSuryawanshi, Sharad, B.1 aUpadhyay, Ankur, K.1 aBhat, Manoj, K.  uhttps://library.ncl.res.in/content/cytotoxic-activity-910-dihydro-25-dimethoxyphenanthrene-17-diol-eulophia-nuda-against-0