02267nas a2200277   4500008004100000022001400041245011900055210006900174260007900243300001200322490000700334520126700341653000801608653002501616653002501641653001201666653001601678653002301694100002501717700002001742700002401762700002501786700002601811700002101837856013101858       2010                        eng d  a1073-608500aRelatedness of Indian flax genotypes (Linum usitatissimum L.): an inter-simple sequence repeat (ISSR) primer assay0 aRelatedness of Indian flax genotypes Linum usitatissimum L an in  a999 RIVERVIEW DRIVE SUITE 208, TOTOWA, NJ 07512 USAbHUMANA PRESS INCcJUN  a161-1700 v453 a<p>The objective of this study was to analyze the genetic relationships, using PCR-based ISSR markers, among 70 Indian flax (Linum usitatissimum L.) genotypes actively utilized in flax breeding programs. Twelve ISSR primers were used for the analysis yielding 136 loci, of which 87 were polymorphic. The average number of amplified loci and the average number of polymorphic loci per primer were 11.3 and 7.25, respectively, while the percent loci polymorphism ranged from 11.1 to 81.8 with an average of 63.9 across all the genotypes. The range of polymorphism information content scores was 0.03-0.49, with an average of 0.18. A dendrogram was generated based on the similarity matrix by the Unweighted Pair Group Method with Arithmetic Mean (UPGMA), wherein the flax genotypes were grouped in five clusters. The Jaccard's similarity coefficient among the genotypes ranged from 0.60 to 0.97. When the omega-3 alpha linolenic acid (ALA) contents of the individual genotypes were correlated with the clusters in the dendrogram, the high ALA containing genotypes were grouped in two clusters. This study identified SLS 50, Ayogi, and Sheetal to be the most diverse genotypes and suggested their use in breeding programs and for developing mapping populations.</p>10aALA10aAlpha linolenic acid10aDiversity assessment10aLinseed10aOil content10aOmega-3 fatty acid1 aRajwade, Ashwini, V.1 aArora, Ritu, S.1 aKadoo, Narendra, Y.1 aHarsulkar, Abhay, M.1 aGhorpade, Prakash, B.1 aGupta, Vidya, S.  uhttps://library.ncl.res.in/content/relatedness-indian-flax-genotypes-linum-usitatissimum-l-inter-simple-sequence-repeat-issr-0