01917nas a2200229   4500008004100000022001400041245011600055210006900171260006700240300001200307490000800319520106400327653001801391653002801409653000801437100002201445700002501467700002301492700002801515700002501543856011901568       2014                        eng d  a0378-109700aIsolation of streptococcus tigurinus - a novel member of streptococcus mitis group from a case of periodontitis0 aIsolation of streptococcus tigurinus a novel member of streptoco  a111 RIVER ST, HOBOKEN 07030-5774, NJ USAbWILEY-BLACKWELLcAUG  a131-1350 v3573 a<p>Streptococcus tigurinus is a new member of the Streptococcus viridians group and is closely related to Streptococcus mitis, Streptococcus pneumoniae, Streptococcus pseudopneumoniae, Streptococcus oralis, and Streptococcus infantis. The type strain AZ_3a(T) of S. tigurinus was originally isolated from a patient with infective endocarditis. Accurate identification of S. tigurinus is facilitated only by newer molecular methods like 16S rRNA gene analysis. During the course of study on bacteraemia and infective endocarditis with reference to periodontitis and viridians group of streptococci, a strain of S. tigurinus isolated from subgingival plaque of a patient with periodontitis identified by 16S rRNA gene analysis, which was originally identified as Streptococcus pluranimalium by Vitek 2. Confirmation by 16S rRNA gene analysis showed 99.39% similarity (1476/1485 bp) with S. tigurinus AZ_3a(T) (AORU01000002). To the best of our knowledge, this is the first report of isolation of S. tigurinus from the oral cavity of a periodontitis patient.</p>10aperiodontitis10aStreptococcus tigurinus10aVGS1 aDhotre, Shree, V.1 aMehetre, Gajanan, T.1 aDharne, Mahesh, S.1 aSuryawanshi, Namdev, M.1 aNagoba, Basavraj, S.  uhttps://library.ncl.res.in/content/isolation-streptococcus-tigurinus-novel-member-streptococcus-mitis-group-case-001684nas a2200253   4500008004100000022001400041245011500055210006900170260007600239300001200315490000600327520077300333653001501106653001501121653002101136653001201157100001801169700002301187700001601210700002501226700002301251700002501274856013101299       2016                        eng d  a1876-034100aDetection of clostridium tetani in human clinical samples using tetX specific primers targeting the neurotoxin0 aDetection of clostridium tetani in human clinical samples using   a84 THEOBALDS RD, LONDON WC1X 8RR, ENGLANDbELSEVIER SCIENCE LONDONcJUL  a105-1090 v93 a<p>Tetanus resulting from ear injury remains an important health problem, particularly in the developing world. We report the successful detection of Clostridium tetani using tetX specific primers targeting the Cl. tetani neurotoxin. The sample was obtained from an ear discharge of a case of otogenic tetanus in a 2-year-old male child. Based on the culture results of the ear discharge, Gram staining and virulence testing by genotyping, a diagnosis of tetanus was confirmed. This is the first report from India on the successful detection of Cl. tetani in a human clinical sample using tetX specific primers targeting the Cl. tetani neurotoxin. (C) 2015 King Saud Bin Abdulaziz University for Health Sciences. Published by Elsevier Limited. All rights reserved.</p>10aCl. tetani10aNeurotoxin10aOtogenic tetanus10aTrismus1 aGanesh, Madhu1 aSheikh, Nasira, K.1 aShah, Pooja1 aMehetre, Gajanan, T.1 aDharne, Mahesh, S.1 aNagoba, Basavraj, S.  uhttps://library.ncl.res.in/content/detection-clostridium-tetani-human-clinical-samples-using-tetx-specific-primers-targeting-001462nas a2200145   4500008004100000245008700041210006900128300001000197490000900207520091800216100002301134700002501157700002201182856011201204       2017                        eng d00aMolecular methods for identification of clostridium tetani by targeting neurotoxin0 aMolecular methods for identification of clostridium tetani by ta  a37-470 v16003 aTetanus is a potentially fatal muscle spasm disease. It is an important public health problem, especially in rural/tribal areas of developing countries. Tetanus toxin, a neurotoxin (tetanospasmin ), is the most important virulence factor that plays a key role in the pathogenicity of tetanus . Confirmation of virulence by confirming the production of tetanospasmin by infecting species forms the most important part in the diagnosis of tetanus . Various molecular methods have been devised for confirmation of diagnosis by targeting different genes. The most common molecular methods are tetanospasmin producing (TetX) gene-targeted methods using TetX-specific primers. Here, we describe various molecular methods targeting TetX gene such as polymerase chain reaction, pulsed-field gel electrophoresis, Southern blotting, loop-mediated isothermal amplification assay, etc. to confirm the virulence of Cl. tetani.1 aDharne, Mahesh, S.1 aNagoba, Basavraj, S.1 aGohil, Kushal, N.  uhttps://library.ncl.res.in/content/molecular-methods-identification-clostridium-tetani-targeting-neurotoxin