01963nas a2200217   4500008004100000022001400041245017400055210006900229260006700298300001000365490000800375520103500383653002401418653004101442653003601483100002501519700002201544700002301566700002401589856013201613       2014                        eng d  a0378-109700aGenome sequencing analysis reveals virulence-related gene content of Ochrobactrum intermedium strain 229E, a urease-positive strain isolated from the human gastric niche0 aGenome sequencing analysis reveals virulencerelated gene content  a111 RIVER ST, HOBOKEN 07030-5774, NJ USAbWILEY-BLACKWELLcOCT  a12-150 v3593 a<p>We report draft genome sequence of Ochrobactrum intermedium strain 229E concurrent with Helicobacter pylori in urease positive gastric biopsy of non-ulcer dyspeptic individual from Southern part of India. Since the role of Ochrobactrum in human gastric environment is poorly understood, comprehensive pathological, microbiological, and genome level understanding are necessary to evaluate its association with H. pylori in the gastric niche. Comparative analysis of O. intermedium 299E strain revealed functional similarities with virulence related gene clusters present in H. pylori genomes, which probably might aid in its ability to persist in the human gastric mucosa. However, H. pylori specific vacuolating cytotoxin (vacA) involved in vacuolization, cytotoxicity, and T-cell inhibition was absent in the O. intermedium 229E genome. Taken together, O. intermedium 229E shared numerous features like secretion system, urease, and flagella with H. pylori genome sequence that might aid concurrence in the gastric niche.</p>10aHelicobacter pylori10aOchrobactrum intermedium strain 229E10avirulence related gene clusters1 aKulkarni, Girish, J.1 aShetty, Sudarshan1 aDharne, Mahesh, S.1 aShouche, Yogesh, S.  uhttps://library.ncl.res.in/content/genome-sequencing-analysis-reveals-virulence-related-gene-content-ochrobactrum-intermedium-000781nas a2200205   4500008004100000022001400041245012100055210006900176260001200245300000900257490000700266100002300273700001800296700002300314700001800337700002300355700004200378700002700420856012800447       2022                        eng d  a0972-232700aGut microbiota dysbiosis in patients with intracranial sino-venous thrombosis and acute ischemic stroke in the young0 aGut microbiota dysbiosis in patients with intracranial sinovenou  cSEP-OCT  a980+0 v251 aPrabhu, Varsha, A.1 aRajput, Vinay1 aYadav, Rakeshkumar1 aGohil, Kushal1 aDharne, Mahesh, S.1 aUnnikrishnan, Mazhuvancherry, Kesavan1 aGorthi, Sankar, Prasad  uhttps://library.ncl.res.in/content/gut-microbiota-dysbiosis-patients-intracranial-sino-venous-thrombosis-and-acute-ischemic02902nas a2200301   4500008004100000022001400041245009800055210006900153260000900222300001100231490000800242520188100250653002502131653002402156653003702180653002702217653001802244653002602262100002502288700002502313700002302338700002302361700001802384700002302402700002202425700002702447856012602474       2024                        eng d  a0168-160500aGenome sequencing and protein modeling unraveled the 2AP biosynthesis in Bacillus cereus DB250 aGenome sequencing and protein modeling unraveled the 2AP biosynt  cMAR   a1106000 v4133 a<p>
	2-Acetyl-1-pyrroline (2AP) is an important and major flavor aroma compound responsible for the fragrance of basmati rice, cheese, wine, and several other food products. Biosynthesis of 2AP in aromatic rice and a few other plant species is associated with a recessive Betaine aldehyde dehydrogenase 2 (BADH2) gene. However, the literature is scant on the relationship between the functional BADH2 gene and 2AP biosynthesis in prokaryotic systems. Therefore, in the present study, we aimed to explore the functionality of the BADH2 gene for 2AP biosynthesis in 2AP synthesizing rice rhizobacterial isolate Bacillus cereus DB25 isolated from the rhizosphere of basmati rice (Oryza sativa L.). Full-length BcBADH2 sequence was obtained through whole genome sequencing (WGS) and further confirmed through traditional PCR and Sanger sequencing. Then the functionality of the BcBADH2 gene was evaluated in-silico through bioinformatics analysis and protein docking studies and further experimentally validated through enzyme assay. The sequencing and bioinformatics analysis results revealed a full-length 1485 bp BcBADH2 coding sequence without any deletion or premature stop codons. Full-length BcBADH2 was found to encode a fully functional protein of 54.08 kDa with pI of 5.22 and showed the presence of the conserved amino acids responsible for enzyme activity. The docking studies confirmed a good affinity between the protein and its substrate whereas the presence of BcBADH2 enzyme activity confirmed the functionality of BADH2 enzyme in B. cereus DB25. In conclusion, the findings of the present study suggest that B. cereus DB25 is able to synthesize 2AP despite a functional BADH2 gene and there may be a different molecular mechanism responsible for 2AP biosynthesis in bacterial systems, unlike that found in aromatic rice and other eukaryotic plant species.</p>
10a2-Acetyl-1-pyrroline10aBasmati rice flavor10aBetaine aldehyde dehydrogenase 210aProtein-ligand docking10aRhizobacteria10aWhole-genome sequence1 aDhondge, Harshal, V.1 aBarvkar, Vitthal, T.1 aDastager, Syed, G.1 aDharne, Mahesh, S.1 aRajput, Vinay1 aPable, Anupama, A.1 aHenry, Robert, J.1 aNadaf, Altafhusain, B.  uhttps://library.ncl.res.in/content/genome-sequencing-and-protein-modeling-unraveled-2ap-biosynthesis-bacillus-cereus-db25