02058nas a2200241 4500008004100000022001400041245011200055210006900167260008100236300000600317490000600323520117100329100002101500700001901521700002301540700002201563700002101585700001901606700002201625700001901647700002001666856013001686 2013 eng d a1757-474900aDraft genome of ochrobactrum intermedium strain M86 isolated from non-ulcer dyspeptic individual from India0 aDraft genome of ochrobactrum intermedium strain M86 isolated fro a236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLANDbBIOMED CENTRAL LTDcAPR a70 v53 a

Background: Ochrobactrum intermedium is an emerging opportunistic pathogen of humans that is closely related to members of the genus Brucella. Earlier, we reported the case of an Indian subject with non-ulcer dyspeptic symptoms whose urease positive gastric biopsy revealed the presence of Helicobacter pylori along with non-Helicobacter like bacteria, eventually cultured and identified as O. intermedium strain M86. Results: Here, we describe the unclosed draft genome of the strain M86 with a length of 5,188,688 bp and mean G+C content of 57.9%. We have also identified many putative gene clusters that might be responsible for its persistence in the gastric mucosa. Comparative analysis of genomic features of Ochrobactrum intermedium strain M86 and Ochrobactrum intermedium LMG 3301(T) was also done. Conclusions: This paper attempts to gain whole-genome based insights into the putative gene determinants of O. intermedium for survival in the highly acidic stomach lumen environment. Identification of genes putatively involved in the various metabolic pathways may lead to a better understanding of the survival of O. intermdedium in acidic condition.

1 aKulkarni, Girish1 aDhotre, Dhiraj1 aDharne, Mahesh, S.1 aShetty, Sudarshan1 aChowdhury, Somak1 aMisra, Vatsala1 aMisra, Sriprakash1 aPatole, Milind1 aShouche, Yogesh uhttps://library.ncl.res.in/content/draft-genome-ochrobactrum-intermedium-strain-m86-isolated-non-ulcer-dyspeptic-individual-001684nas a2200253 4500008004100000022001400041245011500055210006900170260007600239300001200315490000600327520077300333653001501106653001501121653002101136653001201157100001801169700002301187700001601210700002501226700002301251700002501274856013101299 2016 eng d a1876-034100aDetection of clostridium tetani in human clinical samples using tetX specific primers targeting the neurotoxin0 aDetection of clostridium tetani in human clinical samples using  a84 THEOBALDS RD, LONDON WC1X 8RR, ENGLANDbELSEVIER SCIENCE LONDONcJUL a105-1090 v93 a

Tetanus resulting from ear injury remains an important health problem, particularly in the developing world. We report the successful detection of Clostridium tetani using tetX specific primers targeting the Cl. tetani neurotoxin. The sample was obtained from an ear discharge of a case of otogenic tetanus in a 2-year-old male child. Based on the culture results of the ear discharge, Gram staining and virulence testing by genotyping, a diagnosis of tetanus was confirmed. This is the first report from India on the successful detection of Cl. tetani in a human clinical sample using tetX specific primers targeting the Cl. tetani neurotoxin. (C) 2015 King Saud Bin Abdulaziz University for Health Sciences. Published by Elsevier Limited. All rights reserved.

10aCl. tetani10aNeurotoxin10aOtogenic tetanus10aTrismus1 aGanesh, Madhu1 aSheikh, Nasira, K.1 aShah, Pooja1 aMehetre, Gajanan, T.1 aDharne, Mahesh, S.1 aNagoba, Basavraj, S. uhttps://library.ncl.res.in/content/detection-clostridium-tetani-human-clinical-samples-using-tetx-specific-primers-targeting-005367nas a2200205 4500008004100000245017900041210006900220260000800289490000800297520456800305100001904873700001804892700001604910700002304926700002104949700002304970700002304993700002205016856012305038 2020 eng d00aDeciphering taxonomic and functional diversity of fungi as potential bioindicators within confluence stretch of Ganges and Yamuna Rivers, impacted by anthropogenic activities0 aDeciphering taxonomic and functional diversity of fungi as poten cAUG0 v2523 a

River confluences are interesting ecological niche with limited information in respect of the structure and the functions of diverse microbial communities. Fungi are gaining global attention as promising biological spectacles for defining the trophic status of riverine systems. We condense existing knowledge in confluence diversity in two Indian rivers (i.e. Ganges and Yamuna), by combining sediment metagenomics using long read aided MinION nanopore sequencing. A total of 63 OTU’s were observed, of which top 20 OTU’s were considered based on relative abundance of each OTU at a particular location. Fungal genera such as Aspergillus, PenicilliumKluveromyces, Lodderomyces, and Nakaseomyces were deciphered as potential bio indicators of river pollution and eutrophication in the confluent zone. In silico functional gene analysis uncovered hits for neurodegenerative diseases and xenobiotic degradation potential, supporting bioindication of river pollution in wake of anthropogenic intervention.

1 aSamson, Rachel1 aRajput, Vinay1 aShah, Manan1 aYadav, Rakeshkumar1 aSarode, Priyanka1 aDastager, Syed, G.1 aDharne, Mahesh, S.1 aKhairnar, Krishna uhttps://library.ncl.res.in/content/deciphering-taxonomic-and-functional-diversity-fungi-potential-bioindicators-within