02594nas a2200241   4500008004100000022001400041245008200055210006900137260000800206300001400214490000700228520178900235653001502024653002302039653002802062653001702090653005102107100002102158700001902179700001802198700002302216856011302239       2023                        eng d  a2190-681500aInvestigation of cold-active and mesophilic cellulases: opportunities awaited0 aInvestigation of coldactive and mesophilic cellulases opportunit  cJUL  a8829-88520 v133 a<p>
	In the recent decade, the global demand and fuel prices have urged a need to track down an alternate resource. Second-generation (2G) biofuel from the lignocellulosic biomass (LCB) is trending as the fundamental alternative resource. Although LCB is the most abundantly available renewable resource, its commercialization into 2G biofuel technology is a major challenge. Efficient LCB hydrolysis requires a proper lignocellulolytic enzyme cocktail. In view to addressing this problem, several researchers are investigating for efficient enzymes to hydrolyze LCB. To date, there are very few commercial enzymes that aid in the breakdown of LCB, and these enzymes are traditionally isolated from culturable microbes. As only 1% of the microbes can be cultivated in the laboratory, the potentials of the uncultured remain under-explored. In the recent decade, advances in metagenomics using next-generation sequencing (NGS) technologies have revealed the vast diversity of hydrolytic enzymes and multiple domain proteins in the ecosystem. Aiming this, we focus our review on investigating efficient cold-active and mesophilic cellulases from the metagenome. India is an agro-based country with various climatic regions, ranging from warm and humid in the south to mild or moderate and cold or snowy in the Himalayan north; therefore, both cold-active and mesophilic cellulases are needed for LCB to ethanol. Along with downsizing, the conversion cost of LCB to fermentable sugars not only increases the enzymatic conversion but also increases the fermentation efficiency, which ultimately helps to commercialize the second-generation biofuel technology. Metagenomics is an evolving concept, and it has opened new horizons for the discovery of micro-organisms and new enzymes.</p>
10acellulases10aGlycosyl hydrolase10aLignocellulosic biomass10aMetagenomics10asimultaneous saccharification and fermentation1 aChavan, Sambhaji1 aShete, Ashvini1 aMirza, Yasmin1 aDharne, Mahesh, S.  uhttps://library.ncl.res.in/content/investigation-cold-active-and-mesophilic-cellulases-opportunities-awaited02841nas a2200241   4500008004100000022001400041245015000055210006900205260000800274300001200282490000700294520195200301653001402253653002802267653002802295653003302323653002702356653001302383100002502396700002302421700002302444856013202467       2024                        eng d  a0972-152500aBioprocess optimization of penicillium funiculosum NCIM 1228 for improved production and hydrolytic efficiency of cellulases on sugarcane bagasse0 aBioprocess optimization of penicillium funiculosum NCIM 1228 for  cFEB  a215-2330 v263 a<p>The cellulase enzyme is currently the world's third largest commercial enzyme. Because of its requirement in various industries like textiles, food, waste management, pharmaceuticals, agriculture, pulp paper, biofuels, and others, its demand curve has increased sharply. The response surface methodology (RSM) approach was employed to optimize media components and process parameters in the current investigation, which successfully increased the cellulase production from Penicillium funiculosum NCIM 1228. Statistical optimization for the hyperproduction of cellulases was conducted using RSM. The Plackett-Burman design (PBD) approach was used to investigate the critical factors of the cellulase production medium. Subsequently, the Box-Behnken design (BBD) method was used to statistically estimate optimum values and conditions that substantially impacted cellulase production. The estimated optimal combinations of parameters for cellulase production were urea (0.2%), CaCl2 (0.2%), MgSO4 (0.05%), peptone (1.5%), microcrystalline cellulose (5.0%), wheat bran (2.5%), corn steep liquor (CSL) (2.5%), KH2PO4 (0.15%), inoculum (10.65%), agitation (157 rpm), pH (5.88), and temperature (29.84 C-degrees). Conclusively, experimental validation under optimal conditions detected an increased production of 3.82- and 3.61-fold in filter paper assay (FPase) and beta-glucosidase, respectively. Additionally, 1.66- and 1.57-fold enhancement in FPase and beta-glucosidase specific activity was observed where an xylanase activity was enhanced by 3.29-fold. Furthermore, the enzyme showed 51.30 per cent hydrolysis efficiency on sugarcane bagasse lignocellulosic biomass (LCB), at a dose of 7 FPase units per g of cellulose. P. funiculosum NCIM 1228 offers the benefit of producing cellulase with an entire cellulolytic system of enzymes that can be synthesized extracellularly, thus acting as a promising biocatalyst for biofuel industries.</p>
10aCellulase10aLignocellulosic biomass10aPenicillium funiculosum10aResponse surface methodology10aSubmerged fermentation10aXylanase1 aChavan, Sambhaji, B.1 aShete, Ashvini, M.1 aDharne, Mahesh, S.  uhttps://library.ncl.res.in/content/bioprocess-optimization-penicillium-funiculosum-ncim-1228-improved-production-and-hydrolytic