02444nas a2200217   4500008004100000022001400041245005500055210005500110260006100165300001200226490000700238520177700245653001402022653001102036653001302047653002602060653001602086100001602102700001902118856008902137       2007                        eng d  a1054-547600aMicropropagation of mature pongamia pinnata pierre0 aMicropropagation of mature pongamia pinnata pierre  a233 SPRING STREET, NEW YORK, NY 10013 USAbSPRINGERcDEC  a608-6130 v433 a<p>Murashige and Skoog's (MS) basal medium with benzylaminopurine (BA), kinetin (KN), zeatin (Z), and thidiazuron (TDZ) were tested for induction of multiple shoots from mature-tree-derived axillary meristems of Pongamia pinnata. Sprouting of buds was 64% on medium devoid of plant growth regulators (PGR). Incorporation of BA, KN, or Z was ineffective in enhancing sprouting frequency or induction of multiple shoots. Sprouting was completely suppressed in the presence of TDZ. Caulogenic buds appeared in nodal meristems of these explants after withdrawal of TDZ. The number of shoot buds was more on explants precultured in higher concentrations. At higher concentrations of this PGR, a swelling developed at the axil. Multiple shoot primordia appeared and differentiated from this swelling after culturing these explants on MS medium for six passages of 2 wk each. Shoots were harvested and cultured on 0.45 mu M TDZ for further proliferation. Primary explants after harvesting of shoots were identified as `stump'. Reculturing of stumps on 0.45 mu M TDZ produced more shoots. This step was followed for six cycles to obtain additional shoots in each cycle. Shoots maintained on 0.45 mu M TDZ elongated and rooted (70%) on growth regulator-free medium. Rooted shoots (65%) survived transfer to a sand/soil mixture. This report describes the protocol for micropropagation of P. pinnata using mature-tree-derived nodal meristems. Recycling of mature stock to produce a stream of useable shoots for subculturing and eventual stabilization is of great value and can possibly be generalized as an isolation protocol especially for woody species. Repeated proliferation of caulogenic buds from the same origin may also find application in rescue of endangered germplasm.</p>10aBiodiesel10aPongam10aPongamia10ashoot differentiation10athidiazuron1 aSujatha, K.1 aHazra, Sulekha  uhttps://library.ncl.res.in/content/micropropagation-mature-pongamia-pinnata-pierre-0