02008nas a2200229 4500008004100000022001400041245011400055210006900169260010200238300001400340490000600354520110100360653002401461653001701485653002101502653002901523100002201552700002701574700002301601700002601624856012801650 2009 eng d a1930-212600aCellulases from penicillium janthinellum mutants: solid-state production and their stability in ionic liquids0 aCellulases from penicillium janthinellum mutants solidstate prod aCAMPUS BOX 8005, RALEIGH, NC 27695-8005 USAbNORTH CAROLINA STATE UNIV DEPT WOOD & PAPER SCIcAUG a1670-16810 v43 a

The cellulase production by P. janthinellum mutants on lignocellulosic material such as cellulose or steam exploded bagasse (SEB) in combination with wheat bran was studied in solid state fermentation (SSF). One of the mutants, EU2D21, produced the highest levels of endoglucanase (3710 IU g(-1) carbon source) and beta-glucosidase (155 IU g(-1) carbon source). Ionic liquids are so-called green solvents that have become attractive for biocatalysis. Stability of mutant cellulases was tested in 10-50% of the ionic liquid 1-butyl-3-methylimidazolium chloride ([bmim]Cl). FPA and CMCase were significantly stable in 10% ionic liquid after 5h. beta-glucosidase showed 85% of its original activity after 5 h incubation in 30% ionic liquid and retained 55% of its activity after 24 h. This enzyme preparation hydrolyzed ionic-liquid-treated SEB completely in 15 h in the presence of 20% ionic liquid. These studies revealed that there is no need of regenerating cellulose after ionic liquid treatment, since cellulase of mutant strain was found to be significantly stable in the ionic liquid.

10aCellulase stability10aIonic liquid10aMutant cellulase10aSolid state fermentation1 aAdsul, Mukund, G.1 aTerwadkar, Asawari, P.1 aVarma, Anjanikumar1 aGokhale, Digambar, V. uhttps://library.ncl.res.in/content/cellulases-penicillium-janthinellum-mutants-solid-state-production-and-their-stability-001471nas a2200169 4500008004100000022001400041245011000055210006900165260011000234300001400344490000700358520073700365100002201102700001901124700002601143856013201169 2011 eng d a0959-942800aCombined strategy for the dispersion/dissolution of single walled carbon nanotubes and cellulose in water0 aCombined strategy for the dispersiondissolution of single walled aTHOMAS GRAHAM HOUSE, SCIENCE PARK, MILTON RD, CAMBRIDGE CB4 0WF, CAMBS, ENGLANDbROYAL SOC CHEMISTRYcJAN a2054-20560 v213 a

Co-dispersion of native cellulose and single walled carbon nanotubes in water is demonstrated. The pH of the water should be between 6 and 10 for better dispersion. Raman spectra confirm debundling of nanotubes in water and FTIR spectra reveal that the co-solubility is likely caused through disruption of intramolecular hydrogen bonds in the cellulose by hydroxyl groups present on nanotubes surface and the creation of intermolecular hydrogen bonds between cellulose and carbon nanotubes. This is a very simple method for co-dispersion/dissolution of nanotubes and cellulose in water without covalent modifications and expands the repertoire of nanotube modification strategies that are amenable to biological applications.

1 aAdsul, Mukund, G.1 aRey, Diego, A.1 aGokhale, Digambar, V. uhttps://library.ncl.res.in/content/combined-strategy-dispersiondissolution-single-walled-carbon-nanotubes-and-cellulose-water-001852nas a2200217 4500008004100000022001400041245014800055210006900203260009900272300001400371490000800385520094100393653003701334653001901371653002901390653002701419100002001446700002201466700002001488856012601508 2011 eng d a0960-852400aComparative production of cellulases by mutants of penicillium janthinellum NCIM 1171 and its application in hydrolysis of avicel and cellulose0 aComparative production of cellulases by mutants of penicillium j aTHE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLANDbELSEVIER SCI LTDcJUN a6569-65720 v1023 a

Mutants of Penicillium janthinellum NCIM 1171 were evaluated for cellulase production using both submerged fermentation (SmF) and solid state fermentation (SSF). Mutant EU2D-21 gave highest yields of cellulases in both SmF and SSF. Hydrolysis of Avicel and cellulose were compared using SmF and SSF derived enzyme preparations obtained from EU2D-21. Surprisingly, the use of SSF derived preparation gave less hydrolysis compared to SmF derived enzymes. This may be due to inactivation of beta-glucosidase at 50 degrees C in SSF derived enzyme preparations. SmF derived enzyme preparations contained both thermostable and thermosensitive beta-glucosidases where as SSF derived enzyme preparations contained predominantly thermosensitive beta-glucosidase. This is the first report on less thermostability of SSF derived p-glucosidase which is the main reason for getting less hydrolysis. (C) 2011 Elsevier Ltd. All rights reserved.

10abeta-Glucosidase thermostability10aMutant EU2D-2110aSolid state fermentation10aSubmerged fermentation1 aSinghvi, M., S.1 aAdsul, Mukund, G.1 aGokhale, D., V. uhttps://library.ncl.res.in/content/comparative-production-cellulases-mutants-penicillium-janthinellum-ncim-1171-and-its-0