01477nas a2200265   4500008004100000022001400041245008500055210006900140260007700209300001600286490000700302520058100309653001400890653001700904653001800921653000900939653001900948653001700967100001800984700002101002700001901023700002201042700003001064856011701094       2008                        eng d  a0947-653900aNatural gum reduced/stabilized gold nanoparticles for drug delivery formulations0 aNatural gum reducedstabilized gold nanoparticles for drug delive  aPO BOX 10 11 61, D-69451 WEINHEIM, GERMANYbWILEY-V C H VERLAG GMBHcOCT  a10244-102500 v143 a<p>``Gellan Gum'', widely used in food and confectionary industry as a thickening and gelling agent, has been employed as a reducing and stabilizing agent for the synthesis of gold nanoparticles. These nanoparticles display greater stability to electrolyte addition and pH changes relative to the traditional citrate and borohydride reduced nanoparticles. Subsequently these have been used to load anthracycline ring antibiotic doxorubicin hydrochloride. The drug loaded on these nanoparticles showed enhanced cytotoxic effects on human glioma cell lines LN-18 and LN-229.</p>10aApoptosis10aCytotoxicity10adrug delivery10aGold10aNanostructures10anatural gums1 aDhar, Sheetal1 aReddy, Maheswara1 aShiras, Anjali1 aPokharkar, Varsha1 aPrasad, Bhagavatula L. V.  uhttps://library.ncl.res.in/content/natural-gum-reducedstabilized-gold-nanoparticles-drug-delivery-formulations-002071nas a2200265   4500008004100000022001400041245011900055210006900174260009500243300001200338490000600350520109400356653001901450653001301469653002301482653002301505653001601528100002201544700001801566700002001584700001701604700002701621700003001648856012701678       2009                        eng d  a1550-703300aAcute and subacute toxicity studies of chitosan reduced gold nanoparticles: a novel carrier for therapeutic agents0 aAcute and subacute toxicity studies of chitosan reduced gold nan  a25650 NORTH LEWIS WAY, STEVENSON RANCH, CA 91381-1439 USAbAMER SCIENTIFIC PUBLISHERScJUN  a233-2390 v53 a<p>The objective of the present study was to evaluate the oral toxicity of chitosan reduced gold nanoparticles so as to demonstrate its applicability for drug delivery application. Acute oral toxicity studies in female rats documented no deaths or treatment related complications. The LD(50) value of gold nanoparticles was found to be greater than 2000 mg/kg. In case of sub-acute oral toxicity studies, gold nanoparticles were administered orally to male and female rats for a period of 28-days. At the end of study blood samples were collected for haematology and biochemical analysis. For histopathological analysis, organs of animals were weighed and processed for examination. All animals survived the duration of the study, with no significant changes in clinical signs, body weight, food consumption, hematological parameters, organ weights and histopathological findings. These studies establish that chitosan reduced gold nanoparticles produced no treatment related toxicity in rats following oral administration, thus can be exploited for potential therapeutic applications.</p>10aAcute Toxicity10aChitosan10agold nanoparticles10aSub-Acute Toxicity10aWistar Rats1 aPokharkar, Varsha1 aDhar, Sheetal1 aBhumkar, Devika1 aMali, Vishal1 aBodhankar, Subhash, L.1 aPrasad, Bhagavatula L. V.  uhttps://library.ncl.res.in/content/acute-and-subacute-toxicity-studies-chitosan-reduced-gold-nanoparticles-novel-carrier-002299nas a2200265   4500008004100000022001400041245010800055210006900163260007600232300001200308490000700320520135100327653001501678653002001693653001501713653002301728653002201751100001801773700001701791700002701808700001901835700003001854700002201884856012701906       2011                        eng d  a0260-437X00aBiocompatible gellan gum-reduced gold nanoparticles: cellular uptake and subacute oral toxicity studies0 aBiocompatible gellan gumreduced gold nanoparticles cellular upta  aCOMMERCE PLACE, 350 MAIN ST, MALDEN 02148, MA USAbWILEY-BLACKWELLcJUL  a411-4200 v313 a<p>Currently gold nanoparticles are being explored for drug delivery and other biomedical applications; therefore it is necessary to study the fate of such nanoparticles inside the body. The objective of the present study was to investigate the cellular uptake and toxicity of the gold nanoparticles synthesized using a microbial polysaccharide, gellan gum, as a capping and reducing agent. The cellular uptake of gold nanoparticles was studied on mouse embryonic fibroblast cells, NIH3T3 and human glioma cell line, LN-229. The cellular uptake study indicated that the gellan gum-reduced gold nanoparticles were located in cancer cells (LN-229) while no uptake was observed in normal mouse embryonic fibroblast cells (NIH3T3). The toxicity of the gold nanoparticles was evaluated by carrying out subacute 28 day oral toxicity studies in rats. Subacute administration of gum-reduced gold nanoparticles to the rats did not show any hematological or biochemical abnormalities. The weight and normal architecture of various organs did not change compared with control. The current findings, while establishing the specific uptake of nanoparticles into cancerous cells, also demonstrates that the gellan gum-reduced gold nanoparticles are devoid of toxicity in animals following oral administration. Copyright (c) 2010 John Wiley &amp; Sons, Ltd.</p>10acell lines10acellular uptake10aGellan gum10agold nanoparticles10asubacute toxicity1 aDhar, Sheetal1 aMali, Vishal1 aBodhankar, Subhash, L.1 aShiras, Anjali1 aPrasad, Bhagavatula L. V.1 aPokharkar, Varsha  uhttps://library.ncl.res.in/content/biocompatible-gellan-gum-reduced-gold-nanoparticles-cellular-uptake-and-subacute-oral-001712nas a2200205   4500008004100000022001400041245016800055210006900223260011000292300001200402490000600414520082600420100001801246700002101264700002101285700002201306700001901328700003001347856012901377       2011                        eng d  a2040-336400aCytotoxicity of sophorolipid-gellan gum-gold nanoparticle conjugates and their doxorubicin loaded derivatives towards human glioma and human glioma stem cell lines0 aCytotoxicity of sophorolipidgellan gumgold nanoparticle conjugat  aTHOMAS GRAHAM HOUSE, SCIENCE PARK, MILTON RD, CAMBRIDGE CB4 0WF, CAMBS, ENGLANDbROYAL SOC CHEMISTRYcJAN  a575-5800 v33 a<p>Biocompatible gold nanoparticles were synthesized by using a naturally occurring gum-Gellan Gum-as a capping and reducing agent. These were further conjugated with sophorolipids which again were accessed through a biochemical transformation of a fatty acid. The cellular uptake of sophorolipid-conjugated gellan gum reduced gold nanoparticles and their cytotoxicity on human glioma cell line LN-229 and human glioma stem cell line HNGC-2 were investigated. Quite surprisingly even the simple sophorolipid-conjugated gellan gum reduced/capped gold nanoparticles showed greater efficacy in killing the glioma cell lines and, gratifyingly, the glioma stem cell lines also. The cytotoxic effects became more prominent once the anti cancer drug doxorubicin hydrochloride was also conjugated to these gold nanoparticles.</p>1 aDhar, Sheetal1 aReddy, Maheswara1 aPrabhune, Asmita1 aPokharkar, Varsha1 aShiras, Anjali1 aPrasad, Bhagavatula L. V.  uhttps://library.ncl.res.in/content/cytotoxicity-sophorolipid-gellan-gum-gold-nanoparticle-conjugates-and-their-doxorubicin-001545nas a2200193   4500008004100000022001400041245011300055210006900168260011000237300001200347490000600359520075400365100001801119700002301137700001501160700001801175700003001193856012801223       2012                        eng d  a2040-336400aGellan gum capped silver nanoparticle dispersions and hydrogels: cytotoxicity and in vitro diffusion studies0 aGellan gum capped silver nanoparticle dispersions and hydrogels   aTHOMAS GRAHAM HOUSE, SCIENCE PARK, MILTON RD, CAMBRIDGE CB4 0WF, CAMBS, ENGLANDbROYAL SOC CHEMISTRYcOCT  a563-5670 v43 a<p>The preparation of highly stable water dispersions of silver nanoparticles using the naturally available gellan gum as a reducing and capping agent is reported. Further, exploiting the gel formation characteristic of gellan gum silver nanoparticle incorporated gels have also been prepared. The optical properties, morphology, zeta potential and long-term stability of the synthesized silver nanoparticles were investigated. The superior stability of the gellan gum-silver nanoparticle dispersions against pH variation and electrolyte addition is revealed. Finally, we studied the cytotoxicity of AgNP dispersions in mouse embryonic fibroblast cells (NIH3T3) and also evaluated the in vitro diffusion of AgNP dispersions/gels across rat skin.</p>1 aDhar, Sheetal1 aMurawala, Priyanka1 aShiras, A.1 aPokharkar, V.1 aPrasad, Bhagavatula L. V.  uhttps://library.ncl.res.in/content/gellan-gum-capped-silver-nanoparticle-dispersions-and-hydrogels-cytotoxicity-and-vitro-0