01985nas a2200193   4500008004100000022001400041245007600055210006900131260007800200300001200278490000700290520129000297100002401587700001901611700002001630700001701650700002301667856010101690       2008                        eng d  a1462-031600aIn vitro regeneration of `Green Sweet' apple via nucellus-raised callus0 aIn vitro regeneration of Green Sweet apple via nucellusraised ca  aINVICTA PRESS, ASHFORD TN24 8HH, KENT, ENGLANDbHEADLEY BROTHERS LTDcJUL  a447-4520 v833 a<p>For the first time, an in vitro regeneration system for `Green Sweet' apple has been developed from nucellus-raised callus. Callus induction (100%) occurred in nucellar explants excised from immature fruit when cultivated on Murashige and Skoog (MS) basal medium, free of plant growth regulators (PGR) and supplemented with 3.0% (w/v) sucrose and 0.8% (w/v) agar. When cultured on MS basal medium supplemented with 10.0 mu M benzyladenine (BA) and 1.0 mu M gibberellic acid (GA(3)), calli showed 88.9 +/- 5.6% shoot regeneration with 4.1 +/- 0.1. shoots per explant, and a mean shoot length of 3.5 +/- 0.2 cm. Elongated shoots produced roots (44.4 +/- 5.6%) when placed in half-strength MS basal medium supplemented with 1.0 mu M indole-3-butyric acid (IBA) and 1.0 mu M a-naphthaleneacetic acid (NAA). Maximum rooting percentage (55.6 +/- 5.6%) was obtained on 0.4% (w/v) agar, while the highest number of roots per shoot (2.8 +/- 0.4 roots) and root length (3.4 +/- 0.2 cm) were recorded on 0.6% (w/v) agar. Acclimatisation of in vitro-raised plantlets resulted in 39.1 % surviving plantlets. The findings of this study indicate that nucellus-raised callus of the apple cultivar `Green Sweet' can efficiently regenerate shoots that can be rooted and successfully acclimatised.</p>1 aJoshi, Soumai, Kant1 aBisht, Vandana1 aDhar, Uppeandra1 aJoshi, Meena1 aBisht, Anil, Kumar  uhttps://library.ncl.res.in/content/vitro-regeneration-green-sweet-apple-nucellus-raised-callus-002260nas a2200193   4500008004100000022001400041245015000055210006900205260007800274300001200352490000700364520144100371100001801812700002001830700002401850700003001874700003301904856012901937       2011                        eng d  a1462-031600aRecurrent somatic embryogenesis and plant regeneration in Angelica glauca Edgew., a critically endangered medicinal plant of the Western Himalaya0 aRecurrent somatic embryogenesis and plant regeneration in Angeli  aINVICTA PRESS, ASHFORD TN24 8HH, KENT, ENGLANDbHEADLEY BROTHERS LTDcSEP  a493-4980 v863 a<p>Secondary somatic embryogenesis and plant regeneration from seedling explants of Angelica glauca, an endangered medicinal plant of the Himalaya, is reported for the first time. Callus was obtained from all the explants tested in the present study (i.e., epicotyls, hypocotyls, and cotyledonary nodes). The highest frequency of callus formation (95.8%) was observed using epicotyl explants on 4.0 mu M 2,4-dichlorophenoxyacetic acid (2,4-D), whereas 70.8% of hypocotyl explants, and 58.3% of cotyledonary nodes produced callus. One-hundred percent embryogenic callus was induced from epicotyl explants in 2.0 mu M 6-benzyladenine (BA) and 2.0 mu M alpha-naphthaleneacetic acid (NAA), together with the maximum number of somatic embryos (34.2 embryos per explant). Cotyledonary nodes did not produce somatic embryos. Histological studies confirmed the induction of somatic embryogenesis. Somatic embryos germinated into plantlets upon transfer to half-strength Murashige and Skoog (MS) medium without added plant growth regulators. We observed 85% survival of these plantlets under field conditions. The development of secondary embryos was also observed when primary embryos were sub-cultured on full-strength MS medium containing 2.0 mu M NAA plus 2.0 mu M BA. This system of recurrent somatic embryogenesis provides a route for gene transfer and also for the large-scale production of this critically endangered medicinal plant.</p>1 aPandey, Meena1 aDhar, Uppeandra1 aSamant, Sher, Singh1 aShirgurkar, Mrudul, Vijay1 aThengane, Shubhada, Ratnakar  uhttps://library.ncl.res.in/content/recurrent-somatic-embryogenesis-and-plant-regeneration-angelica-glauca-edgew-critically-0