01744nas a2200229   4500008004100000022001400041245010400055210006900159260007300228300001400301490000900315520085400324653001601178653002201194653002801216653002701244653003301271100002701304700002901331700002501360856012901385       2011                        eng d  a0021-967300aDetermination of organic acid impurities in lactic acid obtained by fermentation of sugarcane juice0 aDetermination of organic acid impurities in lactic acid obtained  aPO BOX 211, 1000 AE AMSTERDAM, NETHERLANDSbELSEVIER SCIENCE BVcOCT  a7147-71570 v12183 a<p>Lactic acid produced by fermentation process mostly contains a number of aliphatic carboxylic acids as impurities. In this work, carboxylic acid impurities in lactic acid samples from a number of sources were determined at ppm levels. A simple HPLC method was developed that utilized a new generation polar embedded reverse phase, 20 mM phosphate buffer at pH 2.20 (+/- 0.05) and UV detection at 210 nm. The method enabled quantitative analysis of the above acids in lactic acid matrix. The experimental conditions for column temperature, mobile phase pH and flow rate were optimized. A detailed validation of the method was performed for linearity, precision, accuracy, selectivity, limit of detection (LOD), limit of quantitation (LOQ), ruggedness and repeatability and reproducibility (R&amp;R). (C) 2011 Elsevier B.V. All rights reserved.</p>10aLactic acid10aMethod validation10aOrganic acid impurities10aPolar embedded RP-HPLC10aSugarcane juice fermentation1 aQureshi, Mohd, Shadbar1 aBhongale, Sunil, Sitaram1 aThorave, Archana, K.  uhttps://library.ncl.res.in/content/determination-organic-acid-impurities-lactic-acid-obtained-fermentation-sugarcane-juice-0