02093nas a2200217   4500008004100000022001400041245012400055210006900179260008000248300001400328490000700342520122700349653002401576653002701600653002201627653001701649653002301666100002101689700003301710856013201743       2011                        eng d  a0137-588100aInfluence of abiotic elicitation on production of dipyranocoumarins in suspension cultures of calophyllum inophyllum L.0 aInfluence of abiotic elicitation on production of dipyranocoumar  aTIERGARTENSTRASSE 17, D-69121 HEIDELBERG, GERMANYbSPRINGER HEIDELBERGcNOV  a2149-21580 v333 a<p>Effects of elicitation with heavy metals such as copper, cadmium, chromium (abiotic elicitation) and supplementation of CaCl(2) on production of dipyranocoumarins (inophyllums) in suspension cultures of leaf and stem callus of Calophyllum inophyllum were studied. The optimum timing for elicitor introduction was found to be the 10th day after initiating the suspension cultures. Cadmium as abiotic elicitor in suspension cultures of stem callus was found best to elicit maximum production of inophyllums A, C, and calophyllolide while cadmium in suspension cultures of leaf callus was found best for eliciting maximum production of inophyllums B and P. Inophyllum D was the only dipyranocoumarin whose highest production was achieved when 1.0 mM chromium was used as abiotic elicitor in suspension cultures of stem callus. Out of the three abiotic elicitors used, none could result biomass growth. Only incorporation of CaCl(2) in suspension cultures resulted biomass growth. A maximum of 35.26-fold biomass growth was achieved when suspension cultures of stem callus were incorporated with 2.0 mM CaCl(2). CaCl(2) was noted to have no positive influence on production of most of the dipyranocoumarins under study.</p>10aAbiotic elicitation10aCalophyllum inophyllum10adipyranocoumarins10aHeavy metals10aSuspension culture1 aPawar, Kiran, D.1 aThengane, Shubhada, Ratnakar  uhttps://library.ncl.res.in/content/influence-abiotic-elicitation-production-dipyranocoumarins-suspension-cultures-calophyllum-0