02227nas a2200265   4500008004100000022001400041245014100055210006900196260007300265300001200338490000700350520126500357653001201622653001701634653001401651100001701665700002101682700002201703700001801725700001901743700001701762700002701779700002501806856013001831       2015                        eng d  a0141-813000aZinc inhibits glycation induced structural, functional modifications in albumin and protects erythrocytes from glycated albumin toxicity0 aZinc inhibits glycation induced structural functional modificati  aPO BOX 211, 1000 AE AMSTERDAM, NETHERLANDSbELSEVIER SCIENCE BVcAUG  a601-6100 v793 a<p>The present work aims to investigate the concentration and time dependant effect of zinc on the in vitro non enzymatic modifications of albumin by diabetic levels of glucose. Further, preventive and curative effect of zinc was studied by adding zinc before and after initiation of glycation respectively. Glycation of albumin was done at different concentrations of zinc (125, 250 and 500 mu M) at different time intervals (21,28 and 35 days) with appropriate controls. The antiglycation potential of zinc was assessed by estimating different markers of albumin glycation (fructosamines, carbonyls, bound sugar, AGEs), structural modifications (free amino, thiol group, beta amyloid, native PAGE, ANS binding, fluorescence lifetime decay and CD analysis) and functional properties (antioxidant activity, hemolysis). Zinc at highest concentration (500 mu M) significantly reduced modifications of albumin which was comparable to aminoguanidine and also protected secondary and tertiary structure of albumin after 28 days of incubation. Zinc exhibited significant protective effect on erythrocytes by inhibiting hemolysis. Thus the present study indicate preventive mode of albumin glycation inhibition by zinc. (C) 2015 Elsevier B.V. All rights reserved.</p>10aAlbumin10aErythrocytes10aGlycation1 aTupe, Rashmi1 aKulkarni, Amruta1 aAdeshara, Krishna1 aSankhe, Neena1 aShaikh, Shamim1 aDalai, Sayli1 aBhosale, Siddharth, H.1 aGaikwad, Sushama, M.  uhttps://library.ncl.res.in/content/zinc-inhibits-glycation-induced-structural-functional-modifications-albumin-and-protects-0