01831nas a2200181   4500008004100000022001400041245009000055210006900145260011000214300001400324490000600338520108900344100002501433700002101458700002701479700002301506856012001529       2012                        eng d  a1742-206X00aSimple method for incorporating dynamic effects of intronic miRNA mediated regulation0 aSimple method for incorporating dynamic effects of intronic miRN  aTHOMAS GRAHAM HOUSE, SCIENCE PARK, MILTON RD, CAMBRIDGE CB4 0WF, CAMBS, ENGLANDbROYAL SOC CHEMISTRYcAPR  a2145-21520 v83 a<p>The importance of microRNA (miRNA) in modulating gene expression at the post-transcriptional level is well known. Such regulation has been shown to influence the dynamics of several regulatory networks including the cell cycle. In this study we incorporated regulatory effects of intronic miRNA into an existing mathematical model of the cell cycle through the use of an existing `proxy' protein - the host protein. It was observed that the incorporation of intronic miRNA mediated regulation improved the performance of the model resulting in a closer match to experimental results. To test the universality of this approach we compared the effects of intronic miRNA mediated regulation and host protein mediated regulation. Further, we compared miRNA mediated and protein mediated positive and negative feedback regulations of the target protein. We found that the target protein profiles were predominantly similar. These observations show the applicability of our method for incorporating intronic miRNA mediated dynamic effects in models for regulation of gene expression.</p>1 aGokhale, Sucheta, A.1 aHariharan, Manoj1 aBrahmachari, Samir, K.1 aGadgil, Chetan, J.  uhttps://library.ncl.res.in/content/simple-method-incorporating-dynamic-effects-intronic-mirna-mediated-regulation-0