02401nas a2200193   4500008004100000245011600041210006900157260000800226300002600234490000600260520167800266100002401944700001701968700002701985700001902012700002102031700003102052856012402083       2009                        eng d00aHuman blood vessel-derived endothelial progenitors for endothelialization of small diameter vascular prosthesis0 aHuman blood vesselderived endothelial progenitors for endothelia  cNOV  aArticle Number: e77180 v43 aBackground: Coronary bypass graft failure as a result of acute thrombosis and intimal hyperplasia has been the major challenge in surgical procedures involving small-diameter vascular prosthesis. Coating synthetic grafts with patients' own endothelial cells has been suggested to improve the patency rate and overall success of bypass surgeries. 
Methodology/Principal Findings: We isolated endothelial progenitor cells (EPCs) from leftover pieces of human saphenous vein/mammary artery. We demonstrate that EPCs can be expanded to generate millions of cells under low-density culture conditions. Exposure to high-density conditions induces differentiation to endothelial cell phenotype. EPC-derived endothelial cells show expression of CD144(high), CD31, and vWF. We then assessed the ability of differentiated endothelial cells to adhere and grow on small diameter expanded polytetrafluoroethylene (ePTFE) tubings. Since ePTFE tubings are highly hydrophobic, we optimized protocols to introduce hydrophilic groups on luminal surface of ePTFE tubings. We demonstrate here a stepwise protocol that involves introduction of hydrophilic moieties and coating with defined ECM components that support adhesion of endothelial cells, but not of blood platelets. 
Conclusion/Significance: Our data confirms that endothelial progenitors obtained from adult human blood vessels can be expanded in vitro under xenoprotein-free conditions, for potential use in endothelialization of small diameter ePTFE grafts. These endothelialized grafts may represent a promising treatment strategy for improving the clinical outcome of small-caliber vascular grafts in cardiac bypass surgeries.1 aRanjan, Amaresh, K.1 aKumar, Umesh1 aHardikar, Ashutosh, A.1 aPoddar, Pankaj1 aNair, Prabha, D.1 aHardikar, Anandwardhan, A.  uhttps://library.ncl.res.in/content/human-blood-vessel-derived-endothelial-progenitors-endothelialization-small-diameter02188nas a2200277   4500008004100000022001400041245013600055210006900191260011100260300001200371490000600383520117200389653001801561653001801579653000901597653001301606653001801619653001101637100001701648700002401665700002701689700003101716700002001747700001901767856012401786       2012                        eng d  a1573-413700aGreen approach towards size controlled synthesis of biocompatible antibacterial metal nanoparticles in aqueous phase using lysozyme0 aGreen approach towards size controlled synthesis of biocompatibl  aEXECUTIVE STE Y-2, PO BOX 7917, SAIF ZONE, 1200 BR SHARJAH, U ARAB EMIRATESbBENTHAM SCIENCE PUBL LTDcFEB  a130-1400 v83 a<p>Inspired by array of proteins present in nature, we choose lysozyme (hen egg protein) as a model system to synthesize nanoparticles of noble metals to understand the mechanism of interaction as well as to use them for potential applications such as potent antibacterial agents. Lysozyme is a very well studied biomolecule containing aromatic amino acids like tryptophan and tyrosine. Tyrosine has phenoxy group which is considered to be responsible for interacting with the metal ions. Lysozyme can be suitably modified by treatment with N-bromosuccinimide/N-acetylimidazole to obtain tight control over size distribution of nanoparticles. Here we report the direct synthesis of nanocrystals of gold and silver at controlled pH and light conditions without using any known reducing agents. Out of these, synthesis of gold nanoparticles is assisted by the presence of low concentration of Ag+ ions through the galvanic exchange. It is remarkable to note that the structure of protein is not changed drastically as seen by the FTIR studies. As-synthesized lysozyme capped nanoparticles prepared by this method are biocompatible and retain antibacterial property.</p>10aAntibacterial10aBiocompatible10aGold10alysozyme10aNanoparticles10aSilver1 aKumar, Umesh1 aRanjan, Amaresh, K.1 aSharan, Chandrashekhar1 aHardikar, Anandwardhan, A.1 aPundle, Archana1 aPoddar, Pankaj  uhttps://library.ncl.res.in/content/green-approach-towards-size-controlled-synthesis-biocompatible-antibacterial-metal-0