01901nas a2200229   4500008004100000022001400041245010700055210006900162260007600231300001200307490000700319520101300326653002801339653003301367653003101400653003001431100002001461700002101481700001801502700002401520856012701544       2012                        eng d  a0009-429300a4(R/S)-Amino/guanidino-substituted proline peptides: design, synthesis and DNA transfection properties0 a4RSAminoguanidinosubstituted proline peptides design synthesis a  aSCHWARZTORSTRASSE 9, CH-3007 BERN, SWITZERLANDbSWISS CHEMICAL SOCcDEC  a936-9400 v663 a<p>Collagen is a major structural protein found in the connective tissues of higher organisms and mammals and its biomechanical properties are related to the high thermal stability of its triple helical structure. The primary structure of collagen is composed of the repeating tripeptide motif of Pro-Hyp-Gly, where Hyp is 4R-hydroxy proline. Cationic collagen mimetics consisting of [Pro(X)-Pro(Y)-Gly](6) where Pro(X) and Pro(Y) are 4(R/S)-amino/guanidine proline have been synthesized and shown to form triplexes more stable than the unmodified collagen peptide [Pro-Hyp-Gly](6). The origin of hyperstability is due to conformational pre-organization of proline pucker arising from the electronegativity of the cationic group. These cationic collagen peptides are shown to be effective cell penetrating and plasmid DNA transfecting agents. The results have potential for design of new collagen mimetics for biomaterial applications and efficient cell penetrating agents for drug delivery applications.</p>10a4-Aminoproline peptides10a4-Guanidino proline peptides10aCationic collagen mimetics10aCell penetrating peptides1 aUmashankara, M.1 aNanda, Manaswini1 aSonar, Mahesh1 aGanesh, Krishna, N.  uhttps://library.ncl.res.in/content/4rs-aminoguanidino-substituted-proline-peptides-design-synthesis-and-dna-transfection-001132nas a2200157   4500008004100000022001400041245015400055210006900209260007100278300001400349490000700363520042900370100002100799700002400820856013000844       2012                        eng d  a0022-326300a4(R/S)-Guanidinylprolyl collagen peptides: on-resin synthesis, complexation with plasmid DNA, and the role of peptides in enhancement of transfection0 a4RSGuanidinylprolyl collagen peptides onresin synthesis complexa  a1155 16TH ST, NW, WASHINGTON, DC 20036 USAbAMER CHEMICAL SOCcAPR  a4131-41350 v773 a<p>Chimeric collagen peptides containing cationic 4(R/S)-guanidinylproline are synthesized by in situ amidinylation of 4(R/S)-aminoproline residues. These peptides uniquely enhance the transfection efficiency of GFP-encoded plasmid DNA (pRmHa3-GFP) into cells through efficient DNA condensation resulting from nonspecific electrostatic interactions of cationic guanidino groups and localize in subcytoplasmic organelles.</p>1 aNanda, Manaswini1 aGanesh, Krishna, N.  uhttps://library.ncl.res.in/content/4rs-guanidinylprolyl-collagen-peptides-resin-synthesis-complexation-plasmid-dna-and-role-0