01970nas a2200217   4500008004100000022001400041245006600055210006500121260007100186300001400257490000800271520123400279100002101513700001901534700001801553700002501571700002201596700001901618700001801637856009701655       2012                        eng d  a1520-610600aKinetic studies of amino acid-based surfactant binding to DNA0 aKinetic studies of amino acidbased surfactant binding to DNA  a1155 16TH ST, NW, WASHINGTON, DC 20036 USAbAMER CHEMICAL SOCcMAY  a5831-58370 v1163 a<p>In this work, the binding kinetics of amino acid-based surfactants, presenting different linkers and head groups, with calf thymus (CT)-DNA was studied using stopped-flow fluorescence spectroscopy. The kinetic studies were carried out as a function of Na+ concentration and surfactant-to-DNA charge ratio. The surfactant binding on DNA took place in two consecutive steps, for which the corresponding first and second relative rate constants (k(1) and k(2)) were determined. The fast step was attributed to the surfactant binding to DNA and micelle formation in its vicinity, the slower step to DNA condensation and possible rearrangement of the surfactant aggregates. In general, both relative rate constants increase with surfactant concentration and decrease with the ionic strength of the medium. The architecture of the surfactant was found to have a significant impact on the kinetics of the DNA surfactant complexation. Surfactants with amide linkers showed larger relative rate constants than those with ester linkers. The variation of the relative rate constants with the head groups of the surfactants, alanine and proline, was found to be less obvious, being partially dependent on the surfactant concentration.</p>1 aSanthiya, Deenan1 aDias, Rita, S.1 aDutta, Sounak1 aDas, Prasanta, Kumar1 aMiguel, Maria, G.1 aLindman, Bjorn1 aMaiti, Souvik  uhttps://library.ncl.res.in/content/kinetic-studies-amino-acid-based-surfactant-binding-dna-0