02328nas a2200193 4500008004100000022001400041245009400055210006900149260011000218300001400328490000600342520154600348100002101894700001901915700002401934700002201958700002701980856012702007 2016 eng d a2040-336400aBioresponsive carbon nano-gated multifunctional mesoporous silica for cancer theranostics0 aBioresponsive carbon nanogated multifunctional mesoporous silica aTHOMAS GRAHAM HOUSE, SCIENCE PARK, MILTON RD, CAMBRIDGE CB4 0WF, CAMBS, ENGLANDbROYAL SOC CHEMISTRYcDEC a4537-45460 v83 a

Designing bioresponsive nanocarriers for controlled and efficient intracellular drug release for cancer therapy is a major thrust area in nanomedicine. With recent recognition by the US FDA as a safe material for human trials, mesoporous silica nanoparticles (MSNPs) are being extensively explored as promising theranostic agents. Green fluorescent carbon quantum dots (CQDs), though known as possible alternatives for their more toxic and relatively less efficient predecessors, are less known as gate keepers for drug release control. We report for the first time an efficient bioresponse of CQDs when judiciously designed using glutathione cleavable (redox responsive) disulphide bonds. When the anticancer drug doxorubicin loaded MSNPs are capped with these CQDs, they display promising drug release control on exposure to a mimicked intracellular cancer environment. Their dual functionality is well established with good control on preventing the premature release and exceptional bio-imaging of HeLa cancer cells. Fluorescence images prove selective targeting of HeLa cells by overexpression of folate receptors from the surface functionalised folic acid ligand. Extensive characterisation using XRD, TEM, BET analysis, drug loading tests, drug release kinetics, MTT assay and fluoroscence cell imaging helps in understanding the multi-functionalities of the successful design, extending its scope with exciting prospects towards non-invasive targeted drug delivery and bio-imaging for effective cancer diagnosis and treatment.

1 aPrasad, Rajendra1 aAiyer, Sandhya1 aChauhan, Deepak, S.1 aSrivastava, Rohit1 aSelvaraj, Kaliaperumal uhttps://library.ncl.res.in/content/bioresponsive-carbon-nano-gated-multifunctional-mesoporous-silica-cancer-theranostics-002069nas a2200241 4500008004100000245009300041210006900134260000800203300001600211490000600227520124200233100002401475700002701499700002301526700002301549700002201572700002101594700002901615700002101644700001601665700002201681856012401703 2017 eng d00aNIR light-triggered shrinkable thermoresponsive PNVCL nanoshells for cancer theranostics0 aNIR lighttriggered shrinkable thermoresponsive PNVCL nanoshells  cSEP a44026-440340 v73 aFor the localized treatment of a tumor in a more controlled fashion, several stimuli-responsive nanocarriers and minimally or non-invasive techniques like photothermal therapy (PTT) have emerged. However, PTT is limited to only treatment of small and superficial tumors due to the inability of NIR light to penetrate more and kill the core cells of large and deep-seated tumors. As a preliminary step towards addressing the problem, NIR light-triggered thermoresponsive theranostic nanoshell consisting of chitosan-grafted poly(N-vinyl caprolactam) as core and biocompatible gold as shell (Au PNVCL NS) are synthesized and well characterized by various techniques. PNVCL is polymerized from N-vinyl caprolactam using free radical polymerization method, and chitosan is grafted to raise its lower critical solution temperature (LCST) to hyperthermic temperature (∼43 °C). Surface plasmon resonant gold shell over PNVCL NPs core is assembled by ascorbic acid-driven in situ reduction. Core to shell diameter ratio is controlled to tune the peak in NIR region (750 nm). Therapeutic potential of Au PNVCL NS is determined over breast cancer cells MCF-7, while diagnostic potential is compared with the commercial contrast agent-Omnipaque.1 aChauhan, Deepak, S.1 aSingaravelu, Indulekha1 aGottipalli, Rupesh1 aReddy, Pradeep, K.1 aChikate, Tanmayee1 aGupta, Ramkrishn1 aJahagirdar, Dushyant, N.1 aPrasad, Rajendra1 aDe, Abhijit1 aSrivastava, Rohit uhttps://library.ncl.res.in/content/nir-light-triggered-shrinkable-thermoresponsive-pnvcl-nanoshells-cancer-theranostics01677nas a2200181 4500008004100000245010800041210006900149260000800218300001400226490000700240520100400247100002401251700002101275700002401296700002701320700002201347856012601369 2018 eng d00aDisintegrable NIR light triggered gold nanorods supported liposomal nanohybrids for cancer theranostics0 aDisintegrable NIR light triggered gold nanorods supported liposo cMAY a1510-15180 v293 aIn this work, facile synthesis and application of targeted, dual therapeutic gold nanorods-liposome (GNR-Lipos) nanohybrid for imaging guided photothermal therapy and chemotherapy is investigated. The dual therapeutic GNR-Lipos nanohybrid consists of GNR supported, and doxorubicin (DOX) loaded liposome. GNRs not only serve as a photothermal agent and increase the drug release in intracellular environment of cancer cells, but also provide mechanical strength to liposomes by being decorated both inside and outside of bilayer surfaces. The designed nanohybrid shows a remarkable response for synergistic chemophotothermal therapy compared to only chemotherapy or photothermal therapy. The NIR response, efficient uptake by the cells, disintegration of GNR-Lipos nanohybrid, and synergistic therapeutic effect of photothermal and chemotherapy over breast cancer cells MDA-MB-231 are studied for the better development of a biocompatible nanomaterial based multifunctional cancer theranostic agent.1 aChauhan, Deepak, S.1 aPrasad, Rajendra1 aDevrukhkar, Janhavi1 aSelvaraj, Kaliaperumal1 aSrivastava, Rohit uhttps://library.ncl.res.in/content/disintegrable-nir-light-triggered-gold-nanorods-supported-liposomal-nanohybrids-cancer02261nas a2200181 4500008004100000022001400041245014200055210006900197300001400266490000800280520154500288100002101833700002401854700002401878700002201902700002701924856012801951 2018 eng d a1043-180200aIn vivo examination of folic acid-conjugated gold-silica nanohybrids as contrast agents for localized tumor diagnosis and biodistribution0 aIn vivo examination of folic acidconjugated goldsilica nanohybri a4012-40190 v 293 aEnhanced biocompatibility of nanosized contrast agent with high radiodensity and specific biodistribution is an important parameter for localized tumor imaging and organ safety. Various nanoparticles, especially gold nanorods (GNRs), have been applied for tumor diagnosis. However, their toxicity, nonspecific biodistribution, and easy aggregation are critical issues in cancer medicine. To avoid these issues, encapsulation of the GNRs in the core of nanoscopic mesoporous silica (MS) under ambient conditions, yielding multifunctional nanomaterials for cancer nanomedicine, is a recent and active development. Interestingly, GNR embedded MS nanohybrid (GNR-MS), though a promising material in nanomedicine, is rarely examined for tumor diagnosis, in vivo toxicity, organ safety, contrast ability, and excretion. Herein, we report a systematic in vivo examination of folic acid functionalized GNR-MS (GNR-MS-FA) for localized 4T1 breast tumor diagnosis, organ safety, and excretion using a one-time dose administration. The nanomaterials show good aqueous dispersibility, biocompatibility, high radiodensity, and tumor specific targeting ability (in vitro as well as in vivo). The in vivo tumor diagnosis and specific biodistribution of injected nanomaterials clearly demonstrates their potential for the visualization of tumors deep in the body of mice. In addition, all organs including the healthy glomerulus of the kidney are observed to be free of tissue injuries thereby indicating the superior biocompatibility of the nanomaterials.1 aPrasad, Rajendra1 aAgawane, Sachin, B.1 aChauhan, Deepak, S.1 aSrivastava, Rohit1 aSelvaraj, Kaliaperumal uhttps://library.ncl.res.in/content/vivo-examination-folic-acid-conjugated-gold-silica-nanohybrids-contrast-agents-localized02121nas a2200205 4500008004100000245012900041210006900170260000800239300001600247490000600263520136900269100002101638700002001659700002001679700002401699700002101723700002201744700002701766856012201793 2019 eng d00aGraphene oxide supported liposomes as red emissive theranostics for phototriggered tissue visualization and tumor regression0 aGraphene oxide supported liposomes as red emissive theranostics  cJUL a3312–33200 v23 a

Selective tissue visualization and localized tumor regression without affecting the surrounding healthy tissues are critical concerns in cancer nanomedicine. Importantly, the complete wrapping of a flimsy matrix like liposome by multifunctional graphene oxide is an interesting engineering idea for nanomedicine design. Moreover, designing a safe and biodegradable nanohybrid with significant theranostic ability is a current need for targeted combined therapies. Here, we report a comprehensive result of in vivo tumor diagnosis and phototriggered tumor regression using a biodegradable red emissive nanotheranostic system, viz., graphene oxide flakes fortified liposome (GOF-Lipo), functionalized with folic acid (FA): GOF-Lipo-FA. Graphene oxide support enhances the stability of drug-loaded liposomes in an extracellular environment that prevents the premature release of loaded anticancer drug from the liposomal cavity. Promising outcomes of tumor regression (∼300 to 25 mm3) from organized cellular and animal studies are demonstrated in this work. These studies reveal superior biocompatibility, deep intracellular localization, 4T1 breast tumor diagnosis, and long time tumor binding ability of an injected emissive nanohybrid. Overall, a single dose of designed multifunctional systems demonstrates the best tumor regression.

1 aPrasad, Rajendra1 aYadav, Amit, S.1 aGorain, Mahadeo1 aChauhan, Deepak, S.1 aKundu, Gopal, C.1 aSrivastava, Rohit1 aSelvaraj, Kaliaperumal uhttps://library.ncl.res.in/content/graphene-oxide-supported-liposomes-red-emissive-theranostics-phototriggered-tissue