02151nas a2200265   4500008004100000022001400041245014400055210006900199260007300268300001200341490000700353520114100360653002401501653002601525653002801551653001701579100002801596700002501624700002301649700002401672700002501696700001901721700001701740856012801757       2007                        eng d  a1381-514800aImmobilization of mushroom polyphenol oxidase on poly(allyl glycidyl ether-co-ethylene glycol dimethacrylate) macroporous beaded copolymers0 aImmobilization of mushroom polyphenol oxidase on polyallyl glyci  aPO BOX 211, 1000 AE AMSTERDAM, NETHERLANDSbELSEVIER SCIENCE BVcOCT  a905-9150 v673 a<p>Functional, macroporous, beaded copolymers containing epoxy groups were synthesized for immobilization of polyphenol oxidase (PPO) from edible mushroom (Agaricus bisporus). The effect of incorporation of two different sets of monomers such as glycidyl methacrylate (GMA) and allyl glycidyl ether (AGE) and the effect of cross-linking agent ethylene glycol dimethacrylate (EGDM) with varying cross-link densities on binding and expression of mushroom PPO activity were studied. The effect of porogen viz. cyclohexanol and hexanol on PPO immobilization was studied. AGE copolymers with hexanol as a porogen were found to give higher binding and expression of PPO activity than GE polymers. Crosslinking of amino groups of enzyme with 5% glutaraldehyde for 6 h gave a stable binding of PPO on AGE-75(Hex) polymer with storage half-life of approximately 25 days. Under optimum conditions, AGE-75(Hex) polymer gave 70.3% of activity yield while percent retention of PPO activity was found to be 83.5%. Immobilized PPO showed a broader pH, higher temperature and excellent storage stability. (c) 2007 Elsevier Ltd. All rights reserved.</p>10across-linking agent10aEnzyme immobilization10aEpoxy-activated support10amushroom PPO1 aVaidya, Bhalchandra, K.1 aKarale, Abhijeet, J.1 aSuthar, Hitesh, K.1 aIngavle, Ganesh, C.1 aPathak, Tara, Sankar1 aPonrathnam, S.1 aNene, Sanjay  uhttps://library.ncl.res.in/content/immobilization-mushroom-polyphenol-oxidase-polyallyl-glycidyl-ether-co-ethylene-glycol-001845nas a2200229   4500008004100000022001400041245013700055210006900192260009900261300001400360490000700374520092000381653002601301653002601327653002801353100002801381700002401409700001901433700002101452700002101473856012101494       2008                        eng d  a0960-852400aImmobilization of Candida rugosa lipase on poly(allyl glycidyl ether-co-thylene glycol dimethacrylate) macroporous polymer particles0 aImmobilization of Candida rugosa lipase on polyallyl glycidyl et  aTHE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLANDbELSEVIER SCI LTDcJUN  a3623-36290 v993 a<p>Macroporous polymer particles containing surface epoxy groups were synthesized for immobilization of Candida rugosa lipase (CRL). The effect of incorporation of two different sets of monomers [allyl glycidyl ether (AGE) and glycidyl methacrylate (GMA)] and the effect of crosslinking density on immobilization of lipase were studied. AGE-co-EGDM polymers gave higher binding and expression of lipase than GMA-co-EGDM polymers. Optimization of immobilization parameters was done with respect to immobilization time and enzyme loading. Amongst AGE-co-EGDM polymer series, AGE-150 polymer found to give maximum lipase activity yield and therefore evaluated for temperature, pH and storage stability. Under optimum conditions, AGE-150 polymer gave 78.40% of activity yield. Immobilized lipase on AGE-150 showed a broader pH, higher temperature and excellent storage stability. (C) 2007 Published by Elsevier Ltd.</p>10aCandida rugosa lipase10aEnzyme immobilization10aEpoxy-activated support1 aVaidya, Bhalchandra, K.1 aIngavle, Ganesh, C.1 aPonrathnam, S.1 aKulkarni, B., D.1 aNene, Sanjay, N.  uhttps://library.ncl.res.in/content/immobilization-candida-rugosa-lipase-polyallyl-glycidyl-ether-co-thylene-glycol-001975nas a2200217   4500008004100000022001400041245013800055210006900193260007300262300001200335490000700347520111400354653002601468653002801494653001901522100002801541700002401569700001901593700002101612856012401633       2012                        eng d  a1381-514800aPoly(allyl glycidyl ether-co-ethylene glycol dimethacrylate) copolymer beads as support for covalent immobilization of L-aminoacylase0 aPolyallyl glycidyl ethercoethylene glycol dimethacrylate copolym  aPO BOX 211, 1000 AE AMSTERDAM, NETHERLANDSbELSEVIER SCIENCE BVcOCT  a687-6940 v723 a<p>Porous epoxy-activated copolymer beads were synthesized as support for the covalent immobilization of Aspergillus melleus L-aminoacylase. Here, a series of copolymer bead were synthesized using either glycidyl methacrylate (GMA) or ally] glycidyl ether (AGE) as monomer units and ethylene glycol dimethacrylate (EGDM) as cross-linking agent. The effect of monomer used and the effect of amount of cross-linking agent on covalent immobilization of aminoacylase were studied. Furthermore, the effect of porogen on immobilization of aminoacylase was also evaluated. AGE-co-EGDM copolymer beads gave higher binding of aminoacylase than GMA-co-EGDM copolymer beads. AGE-co-EGDM copolymer beads synthesized with lauryl alcohol as porogen and having 150% cross-linked density (i.e. AGE-(L)-150) gave maximum enzyme binding. Under optimum conditions, AGE-(L)-150 copolymer beads gave about 130 U/g of aminoacylase activity which corresponds to 72.24% of activity yield. Immobilized aminoacylase showed a broader pH, higher temperature and extended storage stability. (C) 2012 Elsevier Ltd. All rights reserved.</p>10aEnzyme immobilization10aEpoxy-activated support10aL-Aminoacylase1 aVaidya, Bhalchandra, K.1 aIngavle, Ganesh, C.1 aPonrathnam, S.1 aNene, Sanjay, N.  uhttps://library.ncl.res.in/content/polyallyl-glycidyl-ether-co-ethylene-glycol-dimethacrylate-copolymer-beads-support-0