01858nas a2200241   4500008004100000022001400041245006700055210006700122260007300189300001400262490000700276520105200283653003201335653001601367653002701383653001301410653001601423100001901439700001601458700002401474700001901498856009901517       2006                        eng d  a1381-514800aNovel beaded polymers from telechelic methacrylic ether esters0 aNovel beaded polymers from telechelic methacrylic ether esters  aPO BOX 211, 1000 AE AMSTERDAM, NETHERLANDSbELSEVIER SCIENCE BVcOCT  a1087-10960 v663 a<p>A series of telechelic dimethacrylic ether-esters (MEE) were prepared by solventless reaction of alpha,omega-dihydroxy poly(oxytetramethylene) (polytetrahydrofuran, PTHF) with phthalic anhydride and glycidyl methacrylate (GMA). MEE was polymerized with GMA as well as GMA-ethylene dimethacrylate (EGDM) to form porous beads. The terpolymer beads were observed using optical microscopy and SEM and characterized for internal pore volume, equilibrium volume-swelling ratio and dimethyl formamide and aqueous buffer regain. The morphology of the beads was dictated by the mole fraction and molecular weight of MEE in the feed. Porosity was found to increase with increase in molecular weight of MEE. Thus, porosity as high as 49%, 50% and 55% was observed with MEE of molecular weights 1580, 2580 and 3480, respectively. At a specific terpolymerization feed ratio of monomers, the terpolymers formed transform from gel like structure into a macroporous one, with increase in molecular weight of MEE. (c) 2006 Elsevier B.V. All rights reserved.</p>10aether-ester dimethacrylates10amacroporous10aPore size distribution10aporosity10atelechelics1 aPujari, N., S.1 aTrivedi, J.1 aIngavle, Ganesh, C.1 aPonrathnam, S.  uhttps://library.ncl.res.in/content/novel-beaded-polymers-telechelic-methacrylic-ether-esters-000792nas a2200169   4500008004100000245019200041210006900233260004400302100002400346700001900370700002900389700001500418700001900433700002200452700002500474856012300499       2006                        eng d00aPoly(High Internal Phase Emulsion) of 2-EHA, 2-EHMA and EGDA with naturally occurring phenolic compounds", paper presented at international conference on ?polymers for advanced technology0 aPolyHigh Internal Phase Emulsion of 2EHA 2EHMA and EGDA with nat  aNational Chemical Laboratory, PunecDEC1 aIngavle, Ganesh, C.1 aShaikh, A., A.1 aBhongale, Sunil, Sitaram1 aScaria, S.1 aChavan, Nayaku1 aQureshi, Mohammed1 aPonrathnam, Surendra  uhttps://library.ncl.res.in/content/polyhigh-internal-phase-emulsion-2-eha-2-ehma-and-egda-naturally-occurring-phenolic02151nas a2200265   4500008004100000022001400041245014400055210006900199260007300268300001200341490000700353520114100360653002401501653002601525653002801551653001701579100002801596700002501624700002301649700002401672700002501696700001901721700001701740856012801757       2007                        eng d  a1381-514800aImmobilization of mushroom polyphenol oxidase on poly(allyl glycidyl ether-co-ethylene glycol dimethacrylate) macroporous beaded copolymers0 aImmobilization of mushroom polyphenol oxidase on polyallyl glyci  aPO BOX 211, 1000 AE AMSTERDAM, NETHERLANDSbELSEVIER SCIENCE BVcOCT  a905-9150 v673 a<p>Functional, macroporous, beaded copolymers containing epoxy groups were synthesized for immobilization of polyphenol oxidase (PPO) from edible mushroom (Agaricus bisporus). The effect of incorporation of two different sets of monomers such as glycidyl methacrylate (GMA) and allyl glycidyl ether (AGE) and the effect of cross-linking agent ethylene glycol dimethacrylate (EGDM) with varying cross-link densities on binding and expression of mushroom PPO activity were studied. The effect of porogen viz. cyclohexanol and hexanol on PPO immobilization was studied. AGE copolymers with hexanol as a porogen were found to give higher binding and expression of PPO activity than GE polymers. Crosslinking of amino groups of enzyme with 5% glutaraldehyde for 6 h gave a stable binding of PPO on AGE-75(Hex) polymer with storage half-life of approximately 25 days. Under optimum conditions, AGE-75(Hex) polymer gave 70.3% of activity yield while percent retention of PPO activity was found to be 83.5%. Immobilized PPO showed a broader pH, higher temperature and excellent storage stability. (c) 2007 Elsevier Ltd. All rights reserved.</p>10across-linking agent10aEnzyme immobilization10aEpoxy-activated support10amushroom PPO1 aVaidya, Bhalchandra, K.1 aKarale, Abhijeet, J.1 aSuthar, Hitesh, K.1 aIngavle, Ganesh, C.1 aPathak, Tara, Sankar1 aPonrathnam, S.1 aNene, Sanjay  uhttps://library.ncl.res.in/content/immobilization-mushroom-polyphenol-oxidase-polyallyl-glycidyl-ether-co-ethylene-glycol-002622nas a2200241   4500008004100000022001400041245006900055210006900124260007300193300001200266490000800278520177300286653001802059653003702077653003102114653002002145653002102165100001802186700002402204700002502228700002302253856010402276       2007                        eng d  a1387-181100aLow density porous carrier based conceptual drug delivery system0 aLow density porous carrier based conceptual drug delivery system  aPO BOX 211, 1000 AE AMSTERDAM, NETHERLANDSbELSEVIER SCIENCE BVcMAY  a290-2980 v1023 a<p>Chronotherapy, a new approach for treating pathological conditions, is based on circadian rhythm. Present work conceptualizes a specific technology, based on combining floating and pulsatile principles to develop drug delivery system, intended for chronotherapy in arthritis. This approach was achieved by using low density microporous polypropylene, Accurel MP 1000 (R), as a multiparticulate carrier along with drug of choice ibuprofen. Carrier amount and solvent volume was kept invariant in designing this simple system by adsorbing drug via melting or solvent evaporation using different carrier: drug ratios. In solvent evaporation, methanol (M) and dichloromethane (DCM) were used. Drug loaded multiparticulate system was subjected to various characterization and evaluation parameters showing influence of adsorption process. Drug release study was performed in acidic environment using pH 1.2 HCl IP medium for 6 h to mimic gastric condition for evaluating gastroretention followed by basic environment using appropriate medium as phosphate buffer pH 7.2 IP for 3 h resembling transit. The release pattern showed influence of drug adsorption methods characterized by ever changing pore geometry with total release ranges in acidic medium as 10.7-27.6% and final release as 55.6-88.6%. Present drug delivery system devoid of any additives/excipients influencing drug release show distinct behaviour from other approaches/technologies in chronotherapy by (a) observing desired low drug release (11%) in acidic medium (b) overcoming the limitations of process variables caused by multiple formulation steps (c) reducing time consumption due to single step process (d) can be extended to controlled release also. (C) 2007 Elsevier Inc. All rights reserved.</p>10achronotherapy10afloating pulsatile drug delivery10alow density porous carrier10amelt adsorption10asolvent polarity1 aSher, Praveen1 aIngavle, Ganesh, C.1 aPonrathnam, Surendra1 aPawar, Atmaram, P.  uhttps://library.ncl.res.in/content/low-density-porous-carrier-based-conceptual-drug-delivery-system02676nas a2200241   4500008004100000022001400041245012400055210006900179260007300248300001000321490000800331520175700339653002602096653001202122653002402134653003302158653002402191100001802215700002402233700002502257700002302282856012902305       2007                        eng d  a0378-517300aLow density porous carrier - drug adsorption and release study by response surface methodology using different solvents0 aLow density porous carrier drug adsorption and release study by   aPO BOX 211, 1000 AE AMSTERDAM, NETHERLANDSbELSEVIER SCIENCE BVcFEB  a72-830 v3313 a<p>Low density porous carriers are widely used in the pharmaceutical applications. Response surface methodology, using 3 2 factorial design was used to study drug adsorption on and its release patterns from microporous polypropylene (Accurel MP 1000((R))) in the absence of additives. Ibuprofen, as model drug, was adsorbed on the polymer by solvent evaporation using two organic solvents methanol (M) and dichloromethane (DCM). The amount of carrier (100 mg) and its particle size range (250-350 mu m) were kept invariant while solvent volume (X-1) and drug amount (X-2) were taken as variables. Drug adsorption pattern depended on the type and amount of solvent used. DSC, XRD, FTIR and TGA, predict crystalline nature and physical form of adsorption. SEM showed the penetration and adsorption of the drug in and on the microporous polymer. Accurel NIP 1000((R)) had a pore volume of 1.992 g/cm(3) and surface area of 55.9855 m(2)/g as detected by mercury porosimetery. On drug adsorption, pore volume ranged from 0.413 to 1.198 g/cm(3) for methanol and 0.280-0.759 g/cm(3) for DCM. Similarly surface area was in the range 38.445-25.497 m(2)/g for methanol and 18.710-32.528 m(2)/g for DCM. The drug release was investigated in phosphate buffer pH 7.2. All batches showed excellent in vitro floating property. Drug release was partial with recovery to complete dependent on type and volume of solvent. R 2 values relating to bulk density, pore volume, surface area and drug release at 60, 120 and 180 min were estimated. Effect of solvent properties shows a positive influence on drug adsorption and release. Release profiles of some batches could be considered as gastroretentive drug delivery system. (c) 2006 Elsevier B.V. All rights reserved.</p>10a3(2) factorial design10acarrier10amicroporous polymer10aResponse surface methodology10asolvent evaporation1 aSher, Praveen1 aIngavle, Ganesh, C.1 aPonrathnam, Surendra1 aPawar, Atmaram, P.  uhttps://library.ncl.res.in/content/low-density-porous-carrier-drug-adsorption-and-release-study-response-surface-methodology01492nas a2200289   4500008004100000022001400041245009900055210006900154260013100223300001200354490000700366520045200373653001600825653001400841653001000855653002100865653002200886100001800908700002200926700001700948700002400965700002600989700001801015700002501033700001801058856012601076       2007                        eng d  a0972-853800aMacroporous beads for lipase immobilization: kinetic resolution of a racemic drug intermediate0 aMacroporous beads for lipase immobilization kinetic resolution o  aDIV FRUITS & HORTICULTURAL TECHNOL, INDIAN AGRICULTURAL RESEARCH INST, NEW DELHI, 110 012, INDIAbHORTICULTURAL SOC INDIAcMAR  a174-1940 v223 a<p>{Lipase isolated from Arthrobacter sp. (RRLJ-1, MTCC No. 5125, named ABL), is effective in resolving a wide range of racemic drug intermediates. In this study, ABL was immobilized on a series of synthetic macroporous epoxy copolymers beads with varying pore sizes, surface area and hydrophobicity. Poly(glycidyl methacrylate-co-ethylene dimethacrylate) beads, with 75% crosslink density and 10% of epoxy groups modified with dibutyl amine [</p>10aauxin pulse10acoco-peat10agrape10amicropropagation10aplantlet survival1 aBhushan, Indu1 aParshad, Rajinder1 aQazi, G., N.1 aIngavle, Ganesh, C.1 aJamalpure, Trupti, M.1 aRajan, C., R.1 aPonrathnam, Surendra1 aGupta, V., K.  uhttps://library.ncl.res.in/content/macroporous-beads-lipase-immobilization-kinetic-resolution-racemic-drug-intermediate-000841nas a2200169   4500008004100000245022100041210006900262260002900331100002400360700001800384700003000402700002300432700001900455700004300474700002500517856012900542       2007                        eng d00aRemoval of AS(III) and AS (V) from contaminated water sources by sorption onto novel pei-attached poly(hipe) beads", paper presented at international conference on ?role of analytical chemistry in nuclear technology?0 aRemoval of ASIII and AS V from contaminated water sources by sor  aBARC, Mumbai, IndiacDEC1 aIngavle, Ganesh, C.1 aTayal, Rajeev1 aShaikh, Wasif, Abdul Late1 aChaudhari, Sanjeev1 aChavan, Nayaku1 aRajan, Chelanattukizhakkemadath, Raman1 aPonrathnam, Surendra  uhttps://library.ncl.res.in/content/removal-asiii-and-v-contaminated-water-sources-sorption-novel-pei-attached-polyhipe-beads01845nas a2200229   4500008004100000022001400041245013700055210006900192260009900261300001400360490000700374520092000381653002601301653002601327653002801353100002801381700002401409700001901433700002101452700002101473856012101494       2008                        eng d  a0960-852400aImmobilization of Candida rugosa lipase on poly(allyl glycidyl ether-co-thylene glycol dimethacrylate) macroporous polymer particles0 aImmobilization of Candida rugosa lipase on polyallyl glycidyl et  aTHE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLANDbELSEVIER SCI LTDcJUN  a3623-36290 v993 a<p>Macroporous polymer particles containing surface epoxy groups were synthesized for immobilization of Candida rugosa lipase (CRL). The effect of incorporation of two different sets of monomers [allyl glycidyl ether (AGE) and glycidyl methacrylate (GMA)] and the effect of crosslinking density on immobilization of lipase were studied. AGE-co-EGDM polymers gave higher binding and expression of lipase than GMA-co-EGDM polymers. Optimization of immobilization parameters was done with respect to immobilization time and enzyme loading. Amongst AGE-co-EGDM polymer series, AGE-150 polymer found to give maximum lipase activity yield and therefore evaluated for temperature, pH and storage stability. Under optimum conditions, AGE-150 polymer gave 78.40% of activity yield. Immobilized lipase on AGE-150 showed a broader pH, higher temperature and excellent storage stability. (C) 2007 Published by Elsevier Ltd.</p>10aCandida rugosa lipase10aEnzyme immobilization10aEpoxy-activated support1 aVaidya, Bhalchandra, K.1 aIngavle, Ganesh, C.1 aPonrathnam, S.1 aKulkarni, B., D.1 aNene, Sanjay, N.  uhttps://library.ncl.res.in/content/immobilization-candida-rugosa-lipase-polyallyl-glycidyl-ether-co-thylene-glycol-003050nas a2200289   4500008004100000022001400041245012900055210006900184260009900253300001200352490000700364520194100371653004402312653002202356653002902378653004002407653001902447653001102466100001802477700002202495700002202517700002402539700001802563700002602581700002402607856012902631       2008                        eng d  a1359-511300aLipase enzyme immobilization on synthetic beaded macroporous copolymers for kinetic resolution of chiral drugs intermediates0 aLipase enzyme immobilization on synthetic beaded macroporous cop  aTHE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLANDbELSEVIER SCI LTDcAPR  a321-3300 v433 a<p>Lipase isolated from Arthrobacter sp. (bacterial strain, MTCC No. 5125) at RRL Jammu, being used for various process development. Arthrobacter sp. lipase (ABL) now has been immobilized on synthetic polymers and reused many a times. In this investigation number of various synthetic macroporous alkylated glycidyl epoxy copolymers with varying hydrophobicity, pore volume and surface area were prepared and used for this study. Among all the polymers prepared and used only two epoxy polymers GMA-EGDM 75-20(I) and GMA-EGDM 75-30(I) with particle size in the range of 150-450 nm, epoxy groups 80 and 70%, tertiary amino groups 20 and 30% was found suitable for immobilization of lipase (ABL). Dibutyl amine (DBA) incorporation created an internal pore radii 20-50 nm and hydrophobic microenvironment in both the polymers for binding the enzyme, which led to improvement in stability and enatioselectivity in racemic resolution process especially by binding to one of the isomers. The optimal ABL binding capacity of polymer GMA-EGDM 75-20(I) was 60 units, 34 mg protein and GMA-EGDM 75-30(l) was 36 units, 21 mg protein/g polymer. The immobilized lipase matrices displayed enhanced pH, thermal, organic solvent and long-term storage stability. Both the immobilized enzyme matrices were tested firstly for the hydrolysis of triglycerides using tributyrin as substrate. After testing, both the matrices were reused for racemic resolution of ethyl-3-hydroxy-3-phenyl propanoate (fluoxetine intermediate, an antidepressant drug) and racemic chiral auxiliary, acetyl-1-phenyl ethanol (intermediate of many chiral drugs) for 15 cycles. These immobilized lipase matrices have shown very high stability on recycling, high-enantioselectivity, high conversion and faster recovery of product compare to free enzyme, therefore these matrices may find use in kinetic resolution process developments. (C) 2007 Elsevier Ltd. All rights reserved.</p>10a1-phenyl ethanol and enantioselectivity10achiral resolution10aenantiomeric excess (ee)10aethyl-3-hydroxy-3-phenyl propanoate10aImmobilization10aLipase1 aBhushan, Indu1 aParshad, Rajinder1 aQazi, Gulam, Nabi1 aIngavle, Ganesh, C.1 aRajan, C., R.1 aPonrathnam, Surendera1 aGupta, Vijay, Kumar  uhttps://library.ncl.res.in/content/lipase-enzyme-immobilization-synthetic-beaded-macroporous-copolymers-kinetic-resolution-002674nas a2200253   4500008004100000022001400041245011100055210006900166260005700235300001200292490000700304520174800311653001802059653004402077653003102121653001402152653002102166100001802187700002402205700002502229700001902254700002302273856012402296       2009                        eng d  a1530-993200aModulation and optimization of drug release from uncoated low density porous carrier based delivery system0 aModulation and optimization of drug release from uncoated low de  a233 SPRING ST, NEW YORK, NY 10013 USAbSPRINGERcJUN  a547-5580 v103 a<p>The purpose of this research work was to explore an application of uncoated porous drug carrier prepared by single-step drug adsorption for a delivery system based on integration of floating and pulsatile principles intended for chronotherapy. This objective was achieved by utilizing 3(2) factorial design, solvent volume (X (1)) and drug amount (X (2)) as selected variables, for drug adsorption using solvents, methanol, and dichloromethane (DCM), of varying polarity. Nitrogen adsorption (N(2)), scanning electron microscopy of cross-sections, and atomic force microscopy were done to study adsorption patterns and their effect on release pattern. Drug release study was customized by performing for 6 h in acidic environment to mimic gastroretention followed by basic environment akin to transit phase. Correlation between porous data from mercury and N(2) adsorption was probably studied for the first time. Observed regression analysis values for pore volume, surface area, and drug release indicated the influence of selected variables. Total release range in acidic medium was 12.77-24.57% for methanol, 8.79-15.26% for DCM, and final release of 69.45-92.23% for methanol, and 60.16-99.99% for DCM influenced by varying internal geometries was observed. Present form of drug delivery system devoid of any additives/excipients influencing drug release shows distinct behavior from other approaches/technologies in chronotherapy by (a) observing desired low drug release (8%) in acidic medium, (b) overcoming the limitations of process variables caused by multiple formulation steps and different characteristic polymers, (c) reducing time consumption due to single step process, and (d) extending as controlled/extended release.</p>10achronotherapy10afloating pulsatile drug delivery system10alow density porous carrier10apore data10asolvent polarity1 aSher, Praveen1 aIngavle, Ganesh, C.1 aPonrathnam, Surendra1 aPoddar, Pankaj1 aPawar, Atmaram, P.  uhttps://library.ncl.res.in/content/modulation-and-optimization-drug-release-uncoated-low-density-porous-carrier-based-002426nas a2200265   4500008004100000022001400041245013100055210006900186260005700255300001400312490000700326520142100333653001801754653004401772653003301816653001401849653003701863100001801900700002401918700002501942700002201967700001701989700002302006856013102029       2009                        eng d  a1530-993200aNovel/conceptual floating pulsatile system using high internal phase emulsion based porous material intended for chronotherapy0 aNovelconceptual floating pulsatile system using high internal ph  a233 SPRING ST, NEW YORK, NY 10013 USAbSPRINGERcDEC  a1368-13800 v103 a<p>The aim of the present study was to design a novel/conceptual delivery system using ibuprofen, anticipated for chronotherapy in arthritis with porous material to overcome the formulation limits (multiple steps, polymers, excipients) and to optimize drug loading for a desired release profile suitable for in vitro investigations. The objective of this delivery system lies in the availability of maximum drug amount for absorption in the wee hours as recommended. Drug loading using 3(2) factorial design on porous carrier, synthesized by high internal phase emulsion technique using styrene and divinylbenzene, was done via solvent evaporation using methanol and dichloromethane. The system was evaluated in vitro for drug loading, encapsulation efficiency, and surface characterization by scanning electron, atomic force microscopy, and customized drug release study. This study examined critical parameters such as solvent volume, drug amount, and solvent polarity on investigations related to drug adsorption and release mostly favoring low-polarity solvent dichloromethane. Overall release in all batches ranged 0.98-52% in acidic medium and 71-94% in basic medium. These results exhibit uniqueness in achieving the least drug release of 0.98%, an ideal one, without using any release modifiers, making it distinct from other approaches/technologies for time and controlled release and for chronotherapy.</p>10achronotherapy10afloating pulsatile drug delivery system10ahigh internal phase emulsion10aibuprofen10amultiparticulate porous carriers1 aSher, Praveen1 aIngavle, Ganesh, C.1 aPonrathnam, Surendra1 aBenson, James, R.1 aLi, Nai-Hong1 aPawar, Atmaram, P.  uhttps://library.ncl.res.in/content/novelconceptual-floating-pulsatile-system-using-high-internal-phase-emulsion-based-porous-002517nas a2200217   4500008004100000022001400041245021100055210006900266260007100335300001400406490000700420520157400427100001902001700003102020700002402051700002202075700002402097700003002121700002502151856012302176       2010                        eng d  a0743-746300aAdsorption induced enzyme denaturation: the role of polymer hydrophobicity in adsorption and denaturation of alpha-chymotrypsin on allyl glycidyl ether (AGE)-ethylene glycol dimethacrylate (EGDM) copolymers0 aAdsorption induced enzyme denaturation the role of polymer hydro  a1155 16TH ST, NW, WASHINGTON, DC 20036 USAbAMER CHEMICAL SOCcJAN  a1096-11060 v263 a<p>Effects of changes in hydrophobicity of polymeric support oil structure and activity of alpha-chymotrypsin (E.C.3.4.21.1) have been studied with copolymers of allyl glycidyl ether (AGE) and ethylene glycol dimethacrylate (EGDM) with increasing molar ratio of EGDM to AGE (cross-link density 0.05 to 1.5). The enzyme is readily adsorbed front aqueous buffer at room temperature following Langmuir adsorption isotherms in unexpectedly large amounts (25% w/w). Relative hydrophobicity of the copolymers has been assessed by studying adsorption of naphthalene and Fmoc-methionine by the series of copolymers from aqueous solutions. Polymer hydrophobicity appears to increase linearly oil increasing cross-link density from 0.05 to 0.25. Further increase in cross-link density Causes a decrease in naphthalene binding but has little effect on binding of Fmoc-Met. Binding of alpha-chymotrypsin to these copolymers follow the trend for Fmoc-methionine binding, rather than naphthalene binding, indicating involvement of polar interactions along with hydrophobic interactions during binding of protein to the polymer. The adsorbed enzyme undergoes extensive denaturation (ca. 80%) with loss of both tertiary and secondary structure on contact with the copolymers as revealed by fluorescence, CID and Raman spectra of the adsorbed protein. Comparison of enzyme adsorption behavior with Eupergit C, macroporous Amberlite XAD-2, and XAD-7 Suggests that polar interactions of the EGDM ester functional groups with the protein play a significant role in enzyme denaturation.</p>1 aLahari, Challa1 aJasti, Lakshmi, Swarnalath1 aFadnavis, Nitin, W.1 aSontakke, Kalpana1 aIngavle, Ganesh, C.1 aDeokar, Sarika, Babasaheb1 aPonrathnam, Surendra  uhttps://library.ncl.res.in/content/adsorption-induced-enzyme-denaturation-role-polymer-hydrophobicity-adsorption-and-001975nas a2200217   4500008004100000022001400041245013800055210006900193260007300262300001200335490000700347520111400354653002601468653002801494653001901522100002801541700002401569700001901593700002101612856012401633       2012                        eng d  a1381-514800aPoly(allyl glycidyl ether-co-ethylene glycol dimethacrylate) copolymer beads as support for covalent immobilization of L-aminoacylase0 aPolyallyl glycidyl ethercoethylene glycol dimethacrylate copolym  aPO BOX 211, 1000 AE AMSTERDAM, NETHERLANDSbELSEVIER SCIENCE BVcOCT  a687-6940 v723 a<p>Porous epoxy-activated copolymer beads were synthesized as support for the covalent immobilization of Aspergillus melleus L-aminoacylase. Here, a series of copolymer bead were synthesized using either glycidyl methacrylate (GMA) or ally] glycidyl ether (AGE) as monomer units and ethylene glycol dimethacrylate (EGDM) as cross-linking agent. The effect of monomer used and the effect of amount of cross-linking agent on covalent immobilization of aminoacylase were studied. Furthermore, the effect of porogen on immobilization of aminoacylase was also evaluated. AGE-co-EGDM copolymer beads gave higher binding of aminoacylase than GMA-co-EGDM copolymer beads. AGE-co-EGDM copolymer beads synthesized with lauryl alcohol as porogen and having 150% cross-linked density (i.e. AGE-(L)-150) gave maximum enzyme binding. Under optimum conditions, AGE-(L)-150 copolymer beads gave about 130 U/g of aminoacylase activity which corresponds to 72.24% of activity yield. Immobilized aminoacylase showed a broader pH, higher temperature and extended storage stability. (C) 2012 Elsevier Ltd. All rights reserved.</p>10aEnzyme immobilization10aEpoxy-activated support10aL-Aminoacylase1 aVaidya, Bhalchandra, K.1 aIngavle, Ganesh, C.1 aPonrathnam, S.1 aNene, Sanjay, N.  uhttps://library.ncl.res.in/content/polyallyl-glycidyl-ether-co-ethylene-glycol-dimethacrylate-copolymer-beads-support-0