01521nas a2200217   4500008004100000022001400041245008500055210006900140260011700209300001000326490000600336520066800342653004301010653001901053653002501072653003501097100001601132700001801148700002101166856011601187       2008                        eng d  a0973-626300aImmobilization of penicillin G acylase on amino functionalized mesoporous silica0 aImmobilization of penicillin G acylase on amino functionalized m  aSECTOR A-80, SCHEME NO 54, VIJAY NAGAR, A B ROAD, INDORE, 452 010 MP, INDIAbRESEARCH JOURNAL BIOTECHNOLOGYcNOV  a18-210 v33 a<p>Penicillin G acylase was immobilized on Amino functionalized mesoporous silica by adsorption method. In this paper we present the effect of penicillin G acylase immobilization on amino-functionalized mesoporous silica and the effect of silica as host matrix on enzyme kinetics. The loading capacity of immobilized Penicillin G acylase is 34 mg protein per 0.5 g of SBA-15. About 65% of the protein was adsorbed on mesoporous silica. The stability of penicillin acylase was enhanced after immobilization on mesoporous silica. The adsorbed enzyme is more stable than the soluble form, both to temperature and pH environments and retained 73% of its activity.</p>10aAmino functionalized mesoporous silica10aImmobilization10aPenicillin G acylase10apH and temperature stabilities1 aSridevi, N.1 aPallavi, Shah1 aAsmita, Prabhune  uhttps://library.ncl.res.in/content/immobilization-penicillin-g-acylase-amino-functionalized-mesoporous-silica-001914nas a2200241   4500008004100000022001400041245013400055210006900189260011700258300001000375490000600385520092800391653003801319653002601357653003401383653003101417653002001448100001801468700002001486700001501506700002101521856013001542       2008                        eng d  a0973-626300aMedia optimization for the production of cephalosporin C acylase from a novel bacterial source: alcaligenes xylosoxidans MTCC*4910 aMedia optimization for the production of cephalosporin C acylase  aSECTOR A-80, SCHEME NO 54, VIJAY NAGAR, A B ROAD, INDORE, 452 010 MP, INDIAbRESEARCH JOURNAL BIOTECHNOLOGYcFEB  a16-210 v33 a<p>The Gram-negative bacterium Alcaligenes xylosoxidans MTCC *491 has been studied for the production of cephalosporin C acylase (CCA), a pharmaceutically important enzyme. The enzyme hydrolyses cephalosporin C (CPC), a natural product, to 7-aminocephalosporanic acid, a key intermediate in the production of semisynthetic cephalosporin antibiotics. Effect of medium, pH, temperature, carbon source, nitrogen source and incubation time, on the production of CCA was studied. 7.7 IU/ml activity was obtained when Luria Bertani broth (LB) was used as a fermentation medium. Glucose and galactose increased the enzyme activity by 9 % and 22 %, while all the nitrogen sources tested showed a repressive effect. Maximum CCA activity was observed around the 5(th) day of incubation indicating potential role of CCA in secondary metabolism. pH 8.0 and 40 degrees C were found to be the optimum conditions for acylase activity.</p>10aalcaligenes xylosoxidans MTCC*49110acephalosporin C (CPC)10acephalosporin C acylase (CCA)10afermentation carbon source10anitrogen source1 aNupura, Hirve1 aAsmita, Tingare1 aSharat, B.1 aAsmita, Prabhune  uhttps://library.ncl.res.in/content/media-optimization-production-cephalosporin-c-acylase-novel-bacterial-source-alcaligenes-002317nas a2200253   4500008004100000022001400041245010300055210006900158260011700227300001000344490000600354520131300360653002701673653002201700653002101722653003501743653003701778653002701815653003001842100001901872700001901891700002101910856013201931       2012                        eng d  a0973-626300aAntibacterial properties of linolenic sophorolipid and its chemically esterified methyl ester form0 aAntibacterial properties of linolenic sophorolipid and its chemi  aSECTOR A-80, SCHEME NO 54, VIJAY NAGAR, A B ROAD, INDORE, 452 010 MP, INDIAbRESEARCH JOURNAL BIOTECHNOLOGYcAUG  a40-450 v73 a<p>The antibacterial activity of Linolenic SL mixture (containing 80% lactone) and its methyl ester derivative against Gram positive (B. subtilis) and Gram negative (E. coli and P. aeruginosa) bacteria is reported here. Bacterial cultures were treated with increasing concentrations of Linolenic SL mixture and its methyl ester derivative and antibacterial activity was checked at different time-intervals (2, 4 and 6 hrs) using standard dilution micromethod and spread plate method. Decrease in bacterial colonies was observed with increase in concentrations of compounds as well as incubation time but the level of effectiveness varies with the compound and bacteria. The minimum inhibitory concentrations (MIC) of Linolenic SL mixture (LNNSL, containing 80% lactone) against B. subtilis, E. coli and P. aeruginosa were found to be 20, 10 and 10 mu g ml(-1) respectively. The MIC values of methyl ester form (LNNSLME) against B. subtilis, E. coli and P. aeruginosa were determined to be &gt;20, 20 and 20 mu g ml(-1) respectively. The results suggest that Linolenic SL mixture (containing 80% lactone) as compared to its methyl ester derivative showed good antibacterial activity towards both the Gram positive and Gram negative bacteria and were found to be more potent against Gram negative bacteria.</p>10aantibacterial activity10aBacillus subtilis10aEscherichia coli10aLinolenic sophorolipid mixture10aMinimum inhibitory concentration10aPseudomonas aeruginosa10aSophorolipid methyl ester1 aReetika, Gupta1 aKumar, Uma, S.1 aAsmita, Prabhune  uhttps://library.ncl.res.in/content/antibacterial-properties-linolenic-sophorolipid-and-its-chemically-esterified-methyl-ester-002008nas a2200229   4500008004100000022001400041245016100055210006900216260011700285300001000402490000600412520109600418653001501514653001901529653001901548653001401567653001301581653001601594100002401610700002101634856012301655       2012                        eng d  a0973-626300aGellan Gum a novel polysaccharide matrix for immobilization of thermo-tolerant yeast cells with invertase activity: factorial design and rheological studies0 aGellan Gum a novel polysaccharide matrix for immobilization of t  aSECTOR A-80, SCHEME NO 54, VIJAY NAGAR, A B ROAD, INDORE, 452 010 MP, INDIAbRESEARCH JOURNAL BIOTECHNOLOGYcNOV  a81-870 v73 a<p>The microbial extracellular polysaccharide gellan gum has been explored for its application in whole cell immobilization. Preliminary evaluation of the physicochemical properties of gellan gum in the presence of monovalent cation tetramethyl ammonium chloride (TMACl) was carried out. Gellan gum immobilized cells demonstrated better rheological properties than gellan gum. Attempts have been made to reveal the evaluation of gellan gum as a matrix to immobilize a thermotolerant yeast Kluyveromyces marxianus NCYC 2675. A 3(2) factorial design was used to study the simultaneous effect of two variables. The effect of the polymer concentration and TMACl concentration on various dependent variables like gelling temperature, mean particle size and enzyme activity of the yeast cells was studied. A statistical model with a significant interaction term was obtained to predict the results. Further, optimized immobilized system was evaluated with significant storage stability at 10 C and enhanced reusability with maximum conversion of &gt; 90 % when crosslinked with glutaraldehyde.</p>10aGellan gum10aGlutaraldehyde10aImmobilization10ainvertase10aRheology10ayeast cells1 aSulabha, Karandikar1 aAsmita, Prabhune  uhttps://library.ncl.res.in/content/gellan-gum-novel-polysaccharide-matrix-immobilization-thermo-tolerant-yeast-cells-0