02695nas a2200265   4500008004100000022001400041245011400055210006900169260007400238300001400312490000700326520177900333653002302112653001202135653001402147653000902161653001002170100002202180700002302202700002102225700001802246700001702264700002502281856012302306       2012                        eng d  a1380-374300aMicrosatellite mapping identifies TTKST-effective stem rust resistance gene in wheat cultivars VL404 and Janz0 aMicrosatellite mapping identifies TTKSTeffective stem rust resis  aVAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDSbSPRINGERcDEC  a1757-17650 v303 a<p>Wheat cultivar VL404 carries seedling resistance to Puccinia graminis f. sp. tritici pathotype TTKST. Monogenic segregation for seedling resistance was observed in a VL404/WL711 recombinant inbred line population and the resistance locus was temporarily designated SrVL. Bulked segregant analysis using Diversity Arrays Technology markers located SrVL on chromosome 2BL. Detailed simple sequence repeat mapping placed SrVL between gwm120 and wmc175, both at genetic distances of 3.3 cM. Based on adult plant responses of Janz and VL404 in India and Kenya, we expected these cultivars to carry the same gene against TTKST. A subset of Diamondbird/Janz doubled haploid (DH) population showed monogenic segregation, when tested against TTKST and the locus was temporarily named SrJNZ. SrVL-linked markers gwm120 and wmc175 flanked SrJNZ at a similar genetic distance, thereby confirming our hypothesis. Chromosome 2BL carries Sr9, Sr16 and Sr28. Sr9 is a multi-allelic locus and all known alleles of Sr9 and Sr16 are ineffective against TTKSK and its derivatives. A recombination value of 16.7 cM between Sr9g-linked stripe rust resistance gene Yr7 and SrJNZ in Diamondbird/Janz DH population suggested that SrJNZ is not an allele at the Sr9 locus. Based on comparison of published genetic distances between Lr13, Sr9, Sr28 and Sr16 with that observed in this study, we concluded SrVL and SrJNZ to be Sr28. This gene was contributed by a common parent Gabo, which also exhibited resistance against TTKST. Sr28-linked markers gwm120 and wmc175 confirmed the presence of this gene in a high proportion of Australian cultivars that showed stem rust resistance in Kenya. These markers can be used for marker-assisted pyramiding of Sr28 with other stem rust resistance genes.</p>10aDisease resistance10aMarkers10aStem rust10aUg9910aWheat1 aBansal, Urmil, K.1 aZwart, Rebecca, S.1 aBhavani, Sridhar1 aWanyera, Ruth1 aGupta, Vidya1 aBariana, Harbans, S.  uhttps://library.ncl.res.in/content/microsatellite-mapping-identifies-ttkst-effective-stem-rust-resistance-gene-wheat-002233nas a2200313   4500008004100000022001400041245007500055210006900130260007400199300001200273490000700285520122400292653002301516653001401539653003001553653002701583653001001610100002601620700002001646700001601666700001701682700001501699700002101714700002001735700002201755700001801777700001801795856010601813       2013                        eng d  a1380-374300aMolecular mapping of leaf rust resistance gene Lr15 in hexaploid wheat0 aMolecular mapping of leaf rust resistance gene Lr15 in hexaploid  aVAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDSbSPRINGERcMAR  a743-7470 v313 a<p>Leaf rust is a widespread and commonly occurring rust disease of wheat. Genetic resistance is the most economical method of reducing losses due to leaf rust. Lr15 has been shown to be present on wheat chromosome 2D and is reported to be a seedling resistance gene. However, tightly linked markers associated with Lr15 have not been reported to date. To identify molecular markers linked to Lr15, an F-2 mapping population of Thatcher x Thatcher-Lr15 was generated. Available wheat simple sequence repeat markers were utilized in parental screening and polymorphic markers were used to analyze the entire population of 221 plants. Phenotypic evaluations of the F-2-derived F-3 progenies with Puccinia triticina Eriks. pathotype 162A (93R15) confirmed the monogenic inheritance of Lr15. The linkage group representing chromosome 2DS was constructed at LOD 4.0 which revealed the closest flanking markers Xgwm4562 and Xgwm102 at a distance of 3.1 and 9.3 cM, respectively. Furthermore, utilization of these flanking markers in combination has successfully identified wheat lines with or without Lr15. These markers could potentially be useful in gene pyramiding with other genes to enhance rust resistance in wheat.</p>10aDisease resistance10aLeaf rust10aMarker-assisted selection10aSimple sequence repeat10aWheat1 aDholakia, Bhushan, B.1 aRajwade, A., V.1 aHosmani, P.1 aKhan, R., R.1 aChavan, S.1 aReddy, D., M. R.1 aLagu, Meena, D.1 aBansal, Urmil, K.1 aSaini, R., G.1 aGupta, V., S.  uhttps://library.ncl.res.in/content/molecular-mapping-leaf-rust-resistance-gene-lr15-hexaploid-wheat-0