02040nas a2200193   4500008004100000022001400041245013600055210006900191260011000260300001400370490000600384520121700390100002401607700002201631700002501653700002001678700002201698856012601720       2013                        eng d  a2046-206900aSynergistic action of flavin containing NADH dependant azoreductase and cytochrome P450 monooxygenase in azoaromatic mineralization0 aSynergistic action of flavin containing NADH dependant azoreduct  aTHOMAS GRAHAM HOUSE, SCIENCE PARK, MILTON RD, CAMBRIDGE CB4 0WF, CAMBS, ENGLANDbROYAL SOC CHEMISTRYcDEC  a3062-30700 v33 a<p>An alkaliphilic strain Bacillus lentus Bl377 was isolated from contaminated soil of the textile area of Solapur, India. The strain was able to degrade almost 98% of recalcitrant azoic compounds by a mutually regulated process of azoreductase and a monooxygenase system. An enzyme activity study and a periodical carbon monoxide (CO) binding spectra study on a UV-visible spectrophotometer revealed that the intermediate amines formed by typical azoreduction (N=N cleavage), subsequently underwent hydroxylation by the cytochrome P450 monooxygenase (CYP450) system. Azoreductase was purified by chromatographic techniques and characterization by MALDI-TOF substantiated its identity as FMN containing NADH dependent azoreductase of 32 kDa in size. Surprisingly, purified azoreductase showed the highest activity at 80 degrees C and pH 8.0. An increase in the activity of superoxide dismutase after decolorization confirmed the signature of oxidative stress and its involvement in the dismutation of reactive metabolites. Intermediate metabolite analysis by HPLC, GC-MS and FTIR and the removal of total organic carbon (TOC) suggested the azoaromatics' degradation leads to mineralization via a TCA cycle.</p>
1 aOturkar, Chetan, C.1 aOthman, Munif, A.1 aKulkarni, Mahesh, J.1 aMadamwar, Datta1 aGawai, Kachru, R.  uhttps://library.ncl.res.in/content/synergistic-action-flavin-containing-nadh-dependant-azoreductase-and-cytochrome-p450-0