01871nas a2200217 4500008004100000022001400041245012000055210006900175260013100244300000900375490000700384520101500391653001601406653001401422653001001436653002101446653002201467100002001489700001801509856012601527 2007 eng d a0972-853800aEffect of auxin types on in vitro and ex vitro rooting and acclimatization of grapevine as influenced by substrates0 aEffect of auxin types on in vitro and ex vitro rooting and accli aDIV FRUITS & HORTICULTURAL TECHNOL, INDIAN AGRICULTURAL RESEARCH INST, NEW DELHI, 110 012, INDIAbHORTICULTURAL SOC INDIAcMAR a5-110 v643 a

Transferring micropropagated plantlets from in vitro to in vivo conditions is a critical step because plantlets have under-developed cuticle and stomata which result in wilting due to low humidity, fungal attack and bleaching in high light intensities. The resumption of terminal growth indicates successful establishment which is a change from mixotrophy to autotrophy. The survival of Vitis vinifera cvs. 2A-Clone and Red Globe plantlets rooted in vitro was 86.66 and 83.33%, respectively and the established plants showed a well-defined root system. Plantlets rooted in vivo by auxin pulse were easier to transfer and acclimatize with a survival percentage of 96.66 and 79.92 for 2A-Clone and Red Globe, respectively but the rooting was of fibrous type with few main roots and less number of root hairs. Coco-peat in combination with sand and soil was better for quick acclimatization and establishment (97.5 and 95% survival for 2A-Clone and Red Globe respectively) of the micropropagated plantlets.

10aauxin pulse10acoco-peat10agrape10amicropropagation10aplantlet survival1 aBarreto, M., S.1 aNookaraju, A. uhttps://library.ncl.res.in/content/effect-auxin-types-vitro-and-ex-vitro-rooting-and-acclimatization-grapevine-influenced02041nas a2200229 4500008004100000022001400041245011500055210006900170260012300239300001200362490000700374520115000381653001801531653000901549653001801558653002001576100001801596700002001614700002401634700002401658856012901682 2007 eng d a0042-750000aSynergistic effect of CPPU and benzyladenine on embryo rescue in six. stenospermocarpic cultivars of grapevine0 aSynergistic effect of CPPU and benzyladenine on embryo rescue in aINST REBENZUCHTUNG GEILWEILERHOF, D-76833 SIEBELDINGEN, GERMANYbBUNDESANSTALT ZUCHTUNGS FORSCHUNG KULTURPFLANZENcJAN a188-1910 v463 a

In ovulo/embryo culture technique has been used to recover hybrids from seedless grapevines. The present investigation was carried out to study the influence of pre-bloom sprays of N-(2-chloro-4-pyridyl)-N'-phenylurea (CPPU) - a synthetic cytokinin, and N-6-benzyladenine (BA) supplemented in culture media on embryo recovery in six stenospermocarpic grapevine cultivars. The results showed synergistic effect of CPPU and BA on embryo recovery. Though a CPPU spray alone increased embryo recovery in four out of six cultivars tested, the efficiency was enhanced several fold on culture of excised ovules on media supplemented with BA. The percentage of embryo recovery also depended on BA concentration and varied among six cultivars indicating a genotypic influence as well. Germination percentage of rescued embryos varied among the six cultivars and mostly corresponded with embryo recovery. Germinated embryos developed into normal plantlets. Present study demonstrates that spraying of panicles with CPPU and incorporation of BA in the ovule culture medium can enhance the embryo recovery in stenospermocarpic cultivars of grapevine.

10abenzyladenine10aCPPU10aembryo rescue10aseedless grapes1 aNookaraju, A.1 aBarreto, M., S.1 aKaribasappa, G., S.1 aAgrawal, Dinesh, C. uhttps://library.ncl.res.in/content/synergistic-effect-cppu-and-benzyladenine-embryo-rescue-six-stenospermocarpic-cultivars-002839nas a2200301 4500008004100000020002200041022001400063245007100077210006900148260030100217300001200518520163500530653001602165653001402181653002202195653002102217653001902238653001002257100002002267700001802287700002102305700002402326700002402350700001902374700001902393700002302412856010202435 2008 eng d a978-90-6605-268-0 a0567-757200aVariability among vitis vinifera cultivars to in vitro propagation0 aVariability among vitis vinifera cultivars to in vitro propagati aPo Box 500, 3001 Leuven 1, BelgiumbAgr & Processed Food Prod Export Dev Author; Indian Farmers Fertilizer Cooperat Ltd; Maharashtra Agro Industries Dev Corp Ltd; Indian Council Agr Res; Natl Res Ctr Grapes; Federat Indian Chambers Commerce & Ind; Natl Hort Board; Minist Food Proc IndustriecMAY a127-1393 a

Response of grapevines to tissue culture has been reported to be genotype dependent, hence it becomes imperative to optimize culture conditions for newly developed varieties or cultivars, needing a large scale planting but availability of sufficient planting stock is a constraint. In the present study, conditions for in vitro propagation of six popular table grape cultivars have been standardized. Single node stem segments of cultivars 2A-Clone of Thompson Seedless, Red Globe, Crimson Seedless, Thompson Seedless, Flame Seedless and Italia cultured on ten different basal media showed varied percentages of bud break and morphogenetic responses. The percentage of bud break varied among the six cultivars. Direct rooting at basal ends of single node segments was observed in all the cultivars except in Italia. Woody plant medium induced the highest response and Eriksson medium the least in all the cultivars tested. Rooted nodal segments with shoots in axils could be established into whole plants on potting. Six cultivars showed different optimum concentrations of growth regulators for induction of maximum number of multiple shoots in both primary as well as secondary nodal segments. Shoot proliferation could be enhanced by several fold in the majority of cultivars on culture of initial shoot clumps to glass bottles instead of culture tubes. Six cultivars showed significant differences in optimum requirement of nutrients and growth regulators for shoot elongation, in vitro and ex vitro rooting and survival rate. Tissue culture plants of all the six cultivars could establish successfully in a greenhouse.

10abasal media10aGrapevine10agrowth regulators10amicropropagation10aTissue culture10aVitis1 aBarreto, M., S.1 aNookaraju, A.1 aJoglekar, A., M.1 aKaribasappa, G., S.1 aAgrawal, Dinesh, C.1 aAdsule, P., G.1 aSawant, I., S.1 aShikhamany, S., D. uhttps://library.ncl.res.in/content/variability-among-vitis-vinifera-cultivars-vitro-propagation-002271nas a2200229 4500008004100000022001400041245012800055210006900183260007300252300001200325490000700337520138600344653002301730653002001753653003401773653002201807653001901829653002201848100001801870700002401888856012901912 2012 eng d a0254-629900aGenetic homogeneity of in vitro raised plants of grapevine cv. crimson seedless revealed by ISSR and microsatellite markers0 aGenetic homogeneity of in vitro raised plants of grapevine cv cr aPO BOX 211, 1000 AE AMSTERDAM, NETHERLANDSbELSEVIER SCIENCE BVcJAN a302-3060 v783 a

The present study was conducted to test the clonal homogeneity of six month old tissue culture raised plants of grapevine cv. Crimson Seedless using Inter Simple Sequence Repeat (ISSR) and Simple Sequence Repeat (SSR) markers. Visible assessment of these in vitro raised plants maintained in polyhouse did not show any morphological differences among themselves. However, to test the genetic homogeneity of these plants, we screened 50 ISSR primers out of which, 22 primers produced scorable and repeatable bands. These 22 primers were used further for assessing genetic homogeneity of in vitro raised plants of Crimson Seedless. These 22 ISSR primers generated 134 distinct band classes with a total of 3216 scorable bands. All the primers showed uniform banding pattern for all the in vitro raised plants and the mother plant. In case of 5 SSR primers (VS I, VVMD5, VVS2, VMCNG4c8 and VVMD31) used, a total of 288 scorable bands were obtained. The allele sizes ranged from 98 to 254 bp. Allelic composition of 23 in vitro raised plants and the mother plant at 5 SSR loci did not show any polymorphism. The results of the two marker systems in the present study revealed the genetic uniformity among the in vitro raised plants demonstrating the reliability of in vitro propagation system used for the cultivar. (C) 2011 SAAB. Published by Elsevier B.V. All rights reserved.

10aAllele composition10aClonal fidelity10aInter Simple Sequence Repeats10aMonomorphic bands10aTissue culture10aVitis vinifera L.1 aNookaraju, A.1 aAgrawal, Dinesh, C. uhttps://library.ncl.res.in/content/genetic-homogeneity-vitro-raised-plants-grapevine-cv-crimson-seedless-revealed-issr-and-002282nas a2200205 4500008004100000022001400041245010000055210006900155260008200224300001200306490000700318520149900325653001601824653002601840653002201866653002301888100001801911700002401929856012301953 2013 eng d a0042-750000aUse of amino acids for a highly efficient somatic embryogenesis in grapevine `Crimson Seedless'0 aUse of amino acids for a highly efficient somatic embryogenesis  aGEILWEILERHOF, D-76833 SIEBELDINGEN, GERMANYbJKI-INSTITUT REBENZUCHTUNGcJAN a137-1400 v523 a

Somatic embryogenesis influenced by growth regulators and amino acids was studied in in vitro leaves of grapevine `Crimson Seedless'. In vitro leaves of the cultivar were collected from multiple shoot cultures maintained on Murashige and Skoog's (MS) basal medium supplemented with 9 mu M N-6-benzyladenine (BA). Among the growth regulators used, BA at 4.5 mu M induced higher embryogenic response producing more number of somatic embryos per explant. This response was increased with the addition of 5 mu M naphthoxyl-acetic acid (NOA) to 1/2 MS containing 4.5 mu M BA. Further, supplementation of amino acids in the callus induction medium significantly improved the embryogenic response of in vitro leaves. The higher number of explants showing somatic embryo production (55.3 %) and higher number of somatic embryos per explant (15.5 per explant) were recorded with the supplementation of 5 mM phenylalanine to callus induction medium. Primary somatic embryos showed repetitive embryogenesis on % MS medium devoid of growth regulators. Plantlets derived from somatic embryos were transferred to soil-sand-peat mixture (1:1:1 v/v) and hardened plantlets were established in greenhouse with 90 % survival. This somatic embryogenesis system has been successfully used for Agrobacterium-mediated transformation studies in `Crimson Seedless' in our laboratory. To our knowledge, this is the first report on the use of amino acids for the high efficient somatic embryogenesis in grapevine.

10aAmino acids10asomatic embryogenesis10aVitis vinifera L.10a`Crimson Seedless'1 aNookaraju, A.1 aAgrawal, Dinesh, C. uhttps://library.ncl.res.in/content/use-amino-acids-highly-efficient-somatic-embryogenesis-grapevine-crimson-seedless-0