02151nas a2200265 4500008004100000022001400041245014400055210006900199260007300268300001200341490000700353520114100360653002401501653002601525653002801551653001701579100002801596700002501624700002301649700002401672700002501696700001901721700001701740856012801757 2007 eng d a1381-514800aImmobilization of mushroom polyphenol oxidase on poly(allyl glycidyl ether-co-ethylene glycol dimethacrylate) macroporous beaded copolymers0 aImmobilization of mushroom polyphenol oxidase on polyallyl glyci aPO BOX 211, 1000 AE AMSTERDAM, NETHERLANDSbELSEVIER SCIENCE BVcOCT a905-9150 v673 a

Functional, macroporous, beaded copolymers containing epoxy groups were synthesized for immobilization of polyphenol oxidase (PPO) from edible mushroom (Agaricus bisporus). The effect of incorporation of two different sets of monomers such as glycidyl methacrylate (GMA) and allyl glycidyl ether (AGE) and the effect of cross-linking agent ethylene glycol dimethacrylate (EGDM) with varying cross-link densities on binding and expression of mushroom PPO activity were studied. The effect of porogen viz. cyclohexanol and hexanol on PPO immobilization was studied. AGE copolymers with hexanol as a porogen were found to give higher binding and expression of PPO activity than GE polymers. Crosslinking of amino groups of enzyme with 5% glutaraldehyde for 6 h gave a stable binding of PPO on AGE-75(Hex) polymer with storage half-life of approximately 25 days. Under optimum conditions, AGE-75(Hex) polymer gave 70.3% of activity yield while percent retention of PPO activity was found to be 83.5%. Immobilized PPO showed a broader pH, higher temperature and excellent storage stability. (c) 2007 Elsevier Ltd. All rights reserved.

10across-linking agent10aEnzyme immobilization10aEpoxy-activated support10amushroom PPO1 aVaidya, Bhalchandra, K.1 aKarale, Abhijeet, J.1 aSuthar, Hitesh, K.1 aIngavle, Ganesh, C.1 aPathak, Tara, Sankar1 aPonrathnam, S.1 aNene, Sanjay uhttps://library.ncl.res.in/content/immobilization-mushroom-polyphenol-oxidase-polyallyl-glycidyl-ether-co-ethylene-glycol-001845nas a2200229 4500008004100000022001400041245013700055210006900192260009900261300001400360490000700374520092000381653002601301653002601327653002801353100002801381700002401409700001901433700002101452700002101473856012101494 2008 eng d a0960-852400aImmobilization of Candida rugosa lipase on poly(allyl glycidyl ether-co-thylene glycol dimethacrylate) macroporous polymer particles0 aImmobilization of Candida rugosa lipase on polyallyl glycidyl et aTHE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLANDbELSEVIER SCI LTDcJUN a3623-36290 v993 a

Macroporous polymer particles containing surface epoxy groups were synthesized for immobilization of Candida rugosa lipase (CRL). The effect of incorporation of two different sets of monomers [allyl glycidyl ether (AGE) and glycidyl methacrylate (GMA)] and the effect of crosslinking density on immobilization of lipase were studied. AGE-co-EGDM polymers gave higher binding and expression of lipase than GMA-co-EGDM polymers. Optimization of immobilization parameters was done with respect to immobilization time and enzyme loading. Amongst AGE-co-EGDM polymer series, AGE-150 polymer found to give maximum lipase activity yield and therefore evaluated for temperature, pH and storage stability. Under optimum conditions, AGE-150 polymer gave 78.40% of activity yield. Immobilized lipase on AGE-150 showed a broader pH, higher temperature and excellent storage stability. (C) 2007 Published by Elsevier Ltd.

10aCandida rugosa lipase10aEnzyme immobilization10aEpoxy-activated support1 aVaidya, Bhalchandra, K.1 aIngavle, Ganesh, C.1 aPonrathnam, S.1 aKulkarni, B., D.1 aNene, Sanjay, N. uhttps://library.ncl.res.in/content/immobilization-candida-rugosa-lipase-polyallyl-glycidyl-ether-co-thylene-glycol-002244nas a2200265 4500008004100000022001400041245013100055210006900186260005700255300001200312490000600324520126300330653002701593653001201620653001801632653002501650653002201675100002901697700002401726700002801750700002201778700002001800700002601820856013201846 2008 eng d a1931-757300aSulfur nanoparticles synthesis and characterization from H2S gas, using novel biodegradable iron chelates in w/o microemulsion0 aSulfur nanoparticles synthesis and characterization from H2S gas a233 SPRING ST, NEW YORK, NY 10013 USAbSPRINGERcJUN a221-2290 v33 a

Sulfur nanoparticles were synthesized from hazardous H2S gas using novel biodegradable iron chelates in w/o microemulsion system. Fe3+-malic acid chelate (0.05 M aqueous solution) was studied in w/o microemulsion containing cyclohexane, Triton X-100 and n-hexanol as oil phase, surfactant, co-surfactant, respectively, for catalytic oxidation of H2S gas at ambient conditions of temperature, pressure, and neutral pH. The structural features of sulfur nanoparticles have been characterized by X-ray diffraction (XRD), transmission electron microscope (TEM), energy dispersive spectroscopy (EDS), diffused reflectance infra-red Fourier transform technique, and BET surface area measurements. XRD analysis indicates the presence of alpha-sulfur. TEM analysis shows that the morphology of sulfur nanoparticles synthesized in w/o microemulsion system is nearly uniform in size (average particle size 10 nm) and narrow particle size distribution (in range of 5-15 nm) as compared to that in aqueous surfactant systems. The EDS analysis indicated high purity of sulfur (> 99%). Moreover, sulfur nanoparticles synthesized in w/o microemulsion system exhibit higher antimicrobial activity (against bacteria, yeast, and fungi) than that of colloidal sulfur.

10aAntimicrobial activity10aH2S gas10airon chelates10asulfur nanoparticles10aw/o microemulsion1 aDeshpande, Aniruddha, S.1 aKhomane, Ramdas, B.1 aVaidya, Bhalchandra, K.1 aJoshi, Renuka, M.1 aHarle, Arti, S.1 aKulkarni, Bhaskar, D. uhttps://library.ncl.res.in/content/sulfur-nanoparticles-synthesis-and-characterization-h2s-gas-using-novel-biodegradable-iron-002249nas a2200241 4500008004100000022001400041245010900055210006900164260009900233300001400332490000700346520131600353653001801669653002401687653003301711653002101744100002401765700002801789700002201817700002101839700002101860856012601881 2008 eng d a0960-852400aUse of response surface optimization for the production of biosurfactant from rhodococcus spp. MTCC 25740 aUse of response surface optimization for the production of biosu aTHE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLANDbELSEVIER SCI LTDcNOV a7875-78800 v993 a

The production of biosurfactant from Rhodococcus spp. MTCC 2574 was effectively enhanced by response surface methodology (RSM). Rhodococcus spp. MTCC 2574 was selected through screening of seven different Rhodococcus strains. The preliminary screening experiments (one-factor at a time) suggested that carbon source: mannitol, nitrogen source: yeast extract and meat peptone and inducer: n-hexadecane are the critical medium components. The concentrations of these four media components were optimized by using central composite rotatable design (CCRD) of RSM. The adequately high R-2 value (0.947) and F score 19.11 indicated the statistical significance of the model. The optimum medium composition for biosurfactant production was found to contain mannitol (1.6 g/L), yeast extract (6.92 g/L), meat peptone (19.65 g/L), n-hexadecane (63.8 g/L). The crude biosurfactant was obtained from methyl tert-butyl ether extraction. The yield of biosurfactant before and after optimization was 3.2 g/L of and 10.9 g/L, respectively. Thus, RSM has increased the yield of biosurfactant to 3.4-fold. The crude biosurfactant decreased the surface tension of water from 72 mN/m to 30.8 mN/m (at 120 mg L-1) and achieved a critical, micelle concentration (CMC) value of 120 mg L-1. (C) 2008 Published by Elsevier Ltd.

10aBiosurfactant10aMedium optimization10aResponse surface methodology10aRhodococcus spp.1 aMutalik, Snehal, R.1 aVaidya, Bhalchandra, K.1 aJoshi, Renuka, M.1 aDesai, Kiran, M.1 aNene, Sanjay, N. uhttps://library.ncl.res.in/content/use-response-surface-optimization-production-biosurfactant-rhodococcus-spp-mtcc-2574-001976nas a2200253 4500008004100000022001400041245013800055210006900193260008000262300001200342490000700354520098200361653001201343653002401355653003001379653003301409653002901442100002801471700002401499700002201523700002101545700002601566856013001592 2009 eng d a1367-543500aEnhanced production of amidase from Rhodococcus erythropolis MTCC 1526 by medium optimisation using a statistical experimental design0 aEnhanced production of amidase from Rhodococcus erythropolis MTC aTIERGARTENSTRASSE 17, D-69121 HEIDELBERG, GERMANYbSPRINGER HEIDELBERGcMAY a671-6780 v363 a

In the present work, statistical experimental methodology was used to enhance the production of amidase from Rhodococcus erythropolis MTCC 1526. R. erythropolis MTCC 1526 was selected through screening of seven strains of Rhodococcus species. The Placket-Burman screening experiments suggested that sorbitol as carbon source, yeast extract and meat peptone as nitrogen sources, and acetamide as amidase inducer are the most influential media components. The concentrations of these four media components were optimised using a face-centred design of response surface methodology (RSM). The optimum medium composition for amidase production was found to contain sorbitol (5 g/L), yeast extract (4 g/L), meat peptone (2.5 g/L), and acetamide (12.25 mM). Amidase activities before and after optimisation were 157.85 units/g dry cells and 1,086.57 units/g dry cells, respectively. Thus, use of RSM increased production of amidase from R. erythropolis MTCC 1526 by 6.88-fold.

10aAmidase10aMedium optimisation10aPlackett-Burman screening10aResponse surface methodology10aRhodococcus erythropolis1 aVaidya, Bhalchandra, K.1 aMutalik, Snehal, R.1 aJoshi, Renuka, M.1 aNene, Sanjay, N.1 aKulkarni, Bhaskar, D. uhttps://library.ncl.res.in/content/enhanced-production-amidase-rhodococcus-erythropolis-mtcc-1526-medium-optimisation-using-002362nas a2200277 4500008004100000022001400041245014500055210006900200260008000269300001200349490000700361520130400368653003001672653001801702653002201720653002301742653003301765653001601798100002101814700002801835700002101863700002201884700002101906700002601927856013101953 2009 eng d a1367-543500aMedia optimization for biosurfactant production by rhodococcus erythropolis MTCC 2794: artificial intelligence versus a statistical approach0 aMedia optimization for biosurfactant production by rhodococcus e aTIERGARTENSTRASSE 17, D-69121 HEIDELBERG, GERMANYbSPRINGER HEIDELBERGcMAY a747-7560 v363 a

This paper entails a comprehensive study on production of a biosurfactant from Rhodococcus erythropolis MTCC 2794. Two optimization techniques-(1) artificial neural network (ANN) coupled with genetic algorithm (GA) and (2) response surface methodology (RSM)-were used for media optimization in order to enhance the biosurfactant yield by Rhodococcus erythropolis MTCC 2794. ANN and RSM models were developed, incorporating the quantity of four medium components (sucrose, yeast extract, meat peptone, and toluene) as independent input variables and biosurfactant yield [calculated in terms of percent emulsification index (% EI24)] as output variable. ANN-GA and RSM were compared for their predictive and generalization ability using a separate data set of 16 experiments, for which the average quadratic errors were similar to 3 and similar to 6%, respectively. ANN-GA was found to be more accurate and consistent in predicting optimized conditions and maximum yield than RSM. For the ANN-GA model, the values of correlation coefficient and average quadratic error were similar to 0.99 and similar to 3%, respectively. It was also shown that ANN-based models could be used accurately for sensitivity analysis. ANN-GA-optimized media gave about a 3.5-fold enhancement in biosurfactant yield.

10aArtificial neural network10aBiosurfactant10aGenetic algorithm10aMedia optimization10aResponse surface methodology10aRhodococcus1 aPal, Moumita, P.1 aVaidya, Bhalchandra, K.1 aDesai, Kiran, M.1 aJoshi, Renuka, M.1 aNene, Sanjay, N.1 aKulkarni, Bhaskar, D. uhttps://library.ncl.res.in/content/media-optimization-biosurfactant-production-rhodococcus-erythropolis-mtcc-2794-artificial-002415nas a2200253 4500008004100000022001400041245015200055210006900207260006900276300001100345490000700356520145300363653002201816653001501838653001701853653003101870653001401901653001701915100002601932700002601958700002801984700002302012856012602035 2011 eng d a1369-703X00aStrain improvement and statistical media optimization for enhanced erythritol production with minimal by-products from candida magnoliae mutant R230 aStrain improvement and statistical media optimization for enhanc aPO BOX 564, 1001 LAUSANNE, SWITZERLANDbELSEVIER SCIENCE SAcJUL a92-1000 v553 a

Mutants of Candida magnoliae NCIM 3470 were generated by ultra-violet and chemical mutagenesis to enhance erythritol production. The mutants were screened for higher reductase activity on agar plates containing high concentration of glucose and 2,3,5-triphenyl tetrazolium chloride (TTC). One of the mutants named as R23 gave maximum erythritol production, 60.3 g L(-1), compared to 14 g L(-1) of the parent strain. Glucose and yeast extract were identified as critical medium components which decide the ratio of polyols produced, mainly erythritol, mannitol and glycerol. In order to enhance the production of erythritol and to minimize the production of mannitol and glycerol, a four component-five level-three response central-composite-rotatable-design (CCRD) of response surface methodology (RSM) model was used. The optimum medium composition for erythritol production was found to contain (g L(-1)) glucose 238, yeast extract 9.2, KH(2)PO(4), 5.16 and MgSO(4) 0.23. Moreover, erythritol production was studied in a 10 L fermentor in batch and fed-batch mode using RSM optimized medium. In fed-batch fermentation, 87.8 g L(-1) erythritol was produced with 31.1% yield, without formation of any other polyols. Thus present study involving strain improvement followed by media and process optimization resulted in 6.2-fold increase in erythritol production and 3.4-fold increase in the yield. (C) 2011 Elsevier B.V. All rights reserved.

10aCandida magnoliae10aErythritol10aFermentation10aMetabolite over-production10aModelling10aOptimization1 aSavergave, Laxman, S.1 aGadre, Ramchandra, V.1 aVaidya, Bhalchandra, K.1 aNarayanan, Karthik uhttps://library.ncl.res.in/content/strain-improvement-and-statistical-media-optimization-enhanced-erythritol-production-001975nas a2200217 4500008004100000022001400041245013800055210006900193260007300262300001200335490000700347520111400354653002601468653002801494653001901522100002801541700002401569700001901593700002101612856012401633 2012 eng d a1381-514800aPoly(allyl glycidyl ether-co-ethylene glycol dimethacrylate) copolymer beads as support for covalent immobilization of L-aminoacylase0 aPolyallyl glycidyl ethercoethylene glycol dimethacrylate copolym aPO BOX 211, 1000 AE AMSTERDAM, NETHERLANDSbELSEVIER SCIENCE BVcOCT a687-6940 v723 a

Porous epoxy-activated copolymer beads were synthesized as support for the covalent immobilization of Aspergillus melleus L-aminoacylase. Here, a series of copolymer bead were synthesized using either glycidyl methacrylate (GMA) or ally] glycidyl ether (AGE) as monomer units and ethylene glycol dimethacrylate (EGDM) as cross-linking agent. The effect of monomer used and the effect of amount of cross-linking agent on covalent immobilization of aminoacylase were studied. Furthermore, the effect of porogen on immobilization of aminoacylase was also evaluated. AGE-co-EGDM copolymer beads gave higher binding of aminoacylase than GMA-co-EGDM copolymer beads. AGE-co-EGDM copolymer beads synthesized with lauryl alcohol as porogen and having 150% cross-linked density (i.e. AGE-(L)-150) gave maximum enzyme binding. Under optimum conditions, AGE-(L)-150 copolymer beads gave about 130 U/g of aminoacylase activity which corresponds to 72.24% of activity yield. Immobilized aminoacylase showed a broader pH, higher temperature and extended storage stability. (C) 2012 Elsevier Ltd. All rights reserved.

10aEnzyme immobilization10aEpoxy-activated support10aL-Aminoacylase1 aVaidya, Bhalchandra, K.1 aIngavle, Ganesh, C.1 aPonrathnam, S.1 aNene, Sanjay, N. uhttps://library.ncl.res.in/content/polyallyl-glycidyl-ether-co-ethylene-glycol-dimethacrylate-copolymer-beads-support-002089nas a2200241 4500008004100000022001400041245011000055210006900165260007300234300001200307490000700319520116600326653002201492653003501514653001901549653002201568653002601590100002801616700002101644700002901665700002101694856013201715 2012 eng d a1381-117700aPreparation of cross-linked enzyme aggregates of L-aminoacylase via co-aggregation with polyethyleneimine0 aPreparation of crosslinked enzyme aggregates of Laminoacylase vi aPO BOX 211, 1000 AE AMSTERDAM, NETHERLANDSbELSEVIER SCIENCE BVcFEB a184-1910 v743 a

L-Aminoacylase from Aspergillus melleus was co-aggregated with polyethyleneimine and subsequently cross-linked with glutaraldehyde to obtain aminoacylase-polyethyleneimine cross-linked enzyme aggregates (termed as AP-CLEA). Under the optimum conditions, AP-CLEA expressed 74.9% activity recovery and 81.2% aggregation yield. The said method of co-aggregation and cross-linking significantly improved the catalytic stability of L-aminoacylase with respect to temperature and storage. AP-CLEA were employed for enantioselective synthesis of three unnatural amino acids (namely: phenylglycine, homophenylalanine and 2-naphthylalanine) via chiral resolution of their ester-. amide- and N-acetyl derivatives. The enantioselectivity of AP-CLEA was the highest for hydrolysis of amino acid amides; was moderate for hydrolysis of N-acetyl amino acids and was the least for hydrolysis of amino acid esters. Furthermore, AP-CLEA were found to retain more than 92% of the initial activity after five consecutive batches of (RS)-homophenylalanine hydrolysis suggesting an adequate operational stability of the biocatalyst. (C) 2011 Elsevier B.V. All rights reserved.

10achiral resolution10aCross-linked enzyme aggregates10aL-Aminoacylase10aPolyethyleneimine10aUnnatural amino acids1 aVaidya, Bhalchandra, K.1 aKuwar, Suyog, S.1 aGolegaonkar, Sandeep, B.1 aNene, Sanjay, N. uhttps://library.ncl.res.in/content/preparation-cross-linked-enzyme-aggregates-l-aminoacylase-co-aggregation-polyethyleneimine-002192nas a2200241 4500008004100000022001400041245010200055210006900157260005700226300001200283490000700295520130800302653002201610653001501632653001601647653002301663653002701686100002601713700002601739700002801765700002501793856013201818 2013 eng d a1615-759100aTwo-stage fermentation process for enhanced mannitol production using Candida magnoliae mutant R90 aTwostage fermentation process for enhanced mannitol production u a233 SPRING ST, NEW YORK, NY 10013 USAbSPRINGERcFEB a193-2030 v363 a

Mutants of Candida magnoliae NCIM 3470 were generated by treatment of ultra-violet radiations, ethyl methyl sulphonate and N-methyl-N'-nitro-N-nitrosoguanidine. Mutants with higher reductase activity were screened by means of 2,3,5-triphenyl tetrazolium chloride agar plate assay. Among the screened mutants, the mutant R9 produced maximum mannitol (i.e. 46 g l(-1)) in liquid fermentation medium containing 250 g l(-1) glucose and hence was selected for further experiments. Preliminary optimization studies were carried out on shake-flask level which increased the mannitol production to 60 g l(-1) in liquid fermentation medium containing 300 g l(-1) glucose. A two-stage fermentation process comprising of growth phase and production phase was employed. During the growth phase, glucose was supplemented and aerobic conditions were maintained. Thereafter, the production phase was initiated by supplementing fructose and switching to anaerobic conditions by discontinuing aeration and decreasing the speed of agitation. The strategy of two-stage fermentation significantly enhanced the production of mannitol up to 240 g l(-1), which is the highest among all fermentative production processes and corresponds to 81 % yield and 4 g l(-1) h(-1) productivity without formation of any by-product.

10aCandida magnoliae10aD-Mannitol10aMutagenesis10aStrain improvement10aTwo-stage fermentation1 aSavergave, Laxman, S.1 aGadre, Ramchandra, V.1 aVaidya, Bhalchandra, K.1 aJogdand, Vitthal, V. uhttps://library.ncl.res.in/content/two-stage-fermentation-process-enhanced-mannitol-production-using-candida-magnoliae-mutant-0