01663nas a2200253   4500008004100000022001400041245014500055210006900200260007300269300001200342490000700354520072000361653001701081653002001098653001101118653001701129653001701146100002501163700002201188700002501210700002101235700002601256856012701282       2008                        eng d  a1369-703X00aProduction and single step purification of cyclodextrin glycosyltransferase from alkalophilic bacillus firmus by ion exchange chromatography0 aProduction and single step purification of cyclodextrin glycosyl  aPO BOX 211, 1000 AE AMSTERDAM, NETHERLANDSbELSEVIER SCIENCE BVcMAY  a510-5150 v393 a<p>Production and purification of starch digesting cyclodextrin glycosyl transferase (CGTase) from alkalophilic Bacillus firmus was investigated. Fermentation was carried out in 141 bioreactor at 28 degrees C using a medium containing dextrin, yeast extract, peptone, (NH4)H2PO4 and MgSO4.7H(2)O. The extracellular enzyme was concentrated by tangential flow ultrafiltration. The concentrated enzyme was chromatographed using DEAE-sepharose and phenyl sepharose. DEAE-sepharose could be used to purify CGTase in a single step with 23.1 fold purification and 80.6% recovery. The enzyme obtained had homogeneity and the molecular weight was 76 kDa confirmed by SDS-PAGE. (c) 2007 Elsevier B.V. All rights reserved.</p>10aalkalophilic10aBacillus firmus10aCGTase10acyclodextrin10aPurification1 aSavergave, Laman, S.1 aMule, Santosh, S.1 aJogdand, Vitthal, V.1 aNene, Sanjay, N.1 aGadre, Ramchandra, V.  uhttps://library.ncl.res.in/content/production-and-single-step-purification-cyclodextrin-glycosyltransferase-alkalophilic-002420nas a2200241   4500008004100000022001400041245011100055210006900166260005700235300001400292490000700306520155500313653001701868653001101885653002101896653002101917100002101938700002201959700002601981700001702007700002502024856012902049       2015                        eng d  a1615-759100aProduction of polyunsaturated fatty acids in recombinant lipomyces starkeyi through submerged fermentation0 aProduction of polyunsaturated fatty acids in recombinant lipomyc  a233 SPRING ST, NEW YORK, NY 10013 USAbSPRINGERcJUL  a1407-14140 v383 a<p>Long chain polyunsaturated fatty acids (LCPUFA) are known to play an important role in human health and nutrition. Considering the limitation of LCPUFA sources, it is necessary to search new avenues for their production. Oleaginous yeasts are an attractive target for harvesting single cell oil, mainly because of the ease of cultivation with cheaper raw material. Lipomyces starkeyi is one such oleaginous yeast, which can accumulate oil to the extent of 60 % of its biomass and where genetic transformation can be achieved. In our earlier work, Delta 15 desaturase gene (AEP37840) from flax was transformed into L. starkeyi. In the present work, we report optimization of medium for the production of omega-3 enriched oil from this transformed yeast. A basic medium containing 20 g/l glucose as a carbon source and 10 g/l yeast extract as a nitrogen source was used during fermentation. At regular time intervals, glucose was fed to maintain high C:N ratio (65:10) during fermentation. Under the most favorable conditions, dry biomass and total lipid content were 18 and 7.29 g/l, respectively. Prior to genetic transformation, L. starkeyi contained 56.03 mg/l DHA along with 71.4 mg/l EPA and 42.2 mg/l ALA. Genetic engineering of this yeast resulted in a strain that produced 1080 mg/l DHA (17.4 %) along with 74.28 mg/l EPA and 126.72 mg/l ALA possibly through modification of PUFA biosynthetic pathway. To the best of our knowledge, this is a first report of DHA enrichment and opens up avenues for LCPUFA production through L. starkeyi.</p>10aFermentation10aLCPUFA10aLipid production10aOleaginous yeast1 aSalunke, Devyani1 aManglekar, Rupali1 aGadre, Ramchandra, V.1 aNene, Sanjay1 aHarsulkar, Abhay, M.  uhttps://library.ncl.res.in/content/production-polyunsaturated-fatty-acids-recombinant-lipomyces-starkeyi-through-submerged-0