02349nas a2200217 4500008004100000022001400041245011100055210006900166260008000235300001200315490000700327520154200334653001701876653001001893653002501903653001101928653001701939100002101956700002601977856012802003 2010 eng d a1367-543500aEnhanced production of gibberellin A(4) (GA(4)) by a mutant of Gibberella fujikuroi in wheat gluten medium0 aEnhanced production of gibberellin A4 GA4 by a mutant of Gibbere aTIERGARTENSTRASSE 17, D-69121 HEIDELBERG, GERMANYbSPRINGER HEIDELBERGcMAR a297-3060 v373 a

Mutants of Gibberella fujikuroi with different colony characteristics, morphology and pigmentation were generated by exposure to UV radiation. A mutant, Mor-189, was selected based on its short filament length, relatively high gibberellin A(4) (GA(4)) and gibberellin A(3) (GA(3)) production, as well as its lack of pigmentation. Production of GA(4) by Mor-189 was studied using different inorganic and organic nitrogen sources, carbon sources and by maintaining the pH of the fermentation medium using calcium carbonate. Analysis of GA(4) and GA(3) was done by reversed-phase high-performance liquid chromatography and LC-MS. The mutants of G. fujikuroi produced more GA(4) when the pH of the medium was maintained above 5. During shake flask studies, the mutant Mor-189 produced 210 mg l(-1) GA(4) in media containing wheat gluten as the nitrogen source and glucose as the carbon source. Fed-batch fermentation in a 14 l agitated fermenter was performed to evaluate the applicability of the mutant Mor-189 for the production of GA(4). In 7-day fed-batch fermentation, 600 mg l(-1) GA(4) were obtained in the culture filtrate. The concentration of GA(4) and GA(3) combined was 713 mg l(-1), of which GA(4) accounted for 84% of the total gibberellin. These values are substantially higher than those published previously. The present study indicated that, along with maintenance of pH and controlled glucose feeding, the use of wheat gluten as the sole nitrogen source considerably enhances GA(4) production by the mutant Mor-189.

10aFermentation10aGA(4)10aGibberella fujikuroi10aMutant10aWheat gluten1 aLale, Geetanjali1 aGadre, Ramchandra, V. uhttps://library.ncl.res.in/content/enhanced-production-gibberellin-a4-ga4-mutant-gibberella-fujikuroi-wheat-gluten-medium-002415nas a2200253 4500008004100000022001400041245015200055210006900207260006900276300001100345490000700356520145300363653002201816653001501838653001701853653003101870653001401901653001701915100002601932700002601958700002801984700002302012856012602035 2011 eng d a1369-703X00aStrain improvement and statistical media optimization for enhanced erythritol production with minimal by-products from candida magnoliae mutant R230 aStrain improvement and statistical media optimization for enhanc aPO BOX 564, 1001 LAUSANNE, SWITZERLANDbELSEVIER SCIENCE SAcJUL a92-1000 v553 a

Mutants of Candida magnoliae NCIM 3470 were generated by ultra-violet and chemical mutagenesis to enhance erythritol production. The mutants were screened for higher reductase activity on agar plates containing high concentration of glucose and 2,3,5-triphenyl tetrazolium chloride (TTC). One of the mutants named as R23 gave maximum erythritol production, 60.3 g L(-1), compared to 14 g L(-1) of the parent strain. Glucose and yeast extract were identified as critical medium components which decide the ratio of polyols produced, mainly erythritol, mannitol and glycerol. In order to enhance the production of erythritol and to minimize the production of mannitol and glycerol, a four component-five level-three response central-composite-rotatable-design (CCRD) of response surface methodology (RSM) model was used. The optimum medium composition for erythritol production was found to contain (g L(-1)) glucose 238, yeast extract 9.2, KH(2)PO(4), 5.16 and MgSO(4) 0.23. Moreover, erythritol production was studied in a 10 L fermentor in batch and fed-batch mode using RSM optimized medium. In fed-batch fermentation, 87.8 g L(-1) erythritol was produced with 31.1% yield, without formation of any other polyols. Thus present study involving strain improvement followed by media and process optimization resulted in 6.2-fold increase in erythritol production and 3.4-fold increase in the yield. (C) 2011 Elsevier B.V. All rights reserved.

10aCandida magnoliae10aErythritol10aFermentation10aMetabolite over-production10aModelling10aOptimization1 aSavergave, Laxman, S.1 aGadre, Ramchandra, V.1 aVaidya, Bhalchandra, K.1 aNarayanan, Karthik uhttps://library.ncl.res.in/content/strain-improvement-and-statistical-media-optimization-enhanced-erythritol-production-002420nas a2200241 4500008004100000022001400041245011100055210006900166260005700235300001400292490000700306520155500313653001701868653001101885653002101896653002101917100002101938700002201959700002601981700001702007700002502024856012902049 2015 eng d a1615-759100aProduction of polyunsaturated fatty acids in recombinant lipomyces starkeyi through submerged fermentation0 aProduction of polyunsaturated fatty acids in recombinant lipomyc a233 SPRING ST, NEW YORK, NY 10013 USAbSPRINGERcJUL a1407-14140 v383 a

Long chain polyunsaturated fatty acids (LCPUFA) are known to play an important role in human health and nutrition. Considering the limitation of LCPUFA sources, it is necessary to search new avenues for their production. Oleaginous yeasts are an attractive target for harvesting single cell oil, mainly because of the ease of cultivation with cheaper raw material. Lipomyces starkeyi is one such oleaginous yeast, which can accumulate oil to the extent of 60 % of its biomass and where genetic transformation can be achieved. In our earlier work, Delta 15 desaturase gene (AEP37840) from flax was transformed into L. starkeyi. In the present work, we report optimization of medium for the production of omega-3 enriched oil from this transformed yeast. A basic medium containing 20 g/l glucose as a carbon source and 10 g/l yeast extract as a nitrogen source was used during fermentation. At regular time intervals, glucose was fed to maintain high C:N ratio (65:10) during fermentation. Under the most favorable conditions, dry biomass and total lipid content were 18 and 7.29 g/l, respectively. Prior to genetic transformation, L. starkeyi contained 56.03 mg/l DHA along with 71.4 mg/l EPA and 42.2 mg/l ALA. Genetic engineering of this yeast resulted in a strain that produced 1080 mg/l DHA (17.4 %) along with 74.28 mg/l EPA and 126.72 mg/l ALA possibly through modification of PUFA biosynthetic pathway. To the best of our knowledge, this is a first report of DHA enrichment and opens up avenues for LCPUFA production through L. starkeyi.

10aFermentation10aLCPUFA10aLipid production10aOleaginous yeast1 aSalunke, Devyani1 aManglekar, Rupali1 aGadre, Ramchandra, V.1 aNene, Sanjay1 aHarsulkar, Abhay, M. uhttps://library.ncl.res.in/content/production-polyunsaturated-fatty-acids-recombinant-lipomyces-starkeyi-through-submerged-0