01490nas a2200253   4500008004100000022001400041245008400055210006900139260010600208300001400314490000700328520053700335653002800872653002100900653002200921653002300943653002000966100002100986700002301007700002901030700002801059700003701087856011201124       2014                        eng d  a0040-403900aFungi mediated production and practical purification of (R)-(-)-3-quinuclidinol0 aFungi mediated production and practical purification of R3quinuc  aTHE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLANDbPERGAMON-ELSEVIER SCIENCE LTDcOCT  a5911-59140 v553 a<p>A fungal system belonging to Mucoraceae family (Mucor piriformis) was explored for the asymmetric reduction of a prochiral ketone, 3-quinuclidinone (I) in an efficient manner to produce an important pharmaceutical precursor (R)-(-)-3-quinuclidinol (II) with similar to 96% enantiomeric excess. The efficiency of the process was improved by developing a cation exchange resin (Amberlite IR-120) which assisted the purification of water soluble metabolite II from fermentation media. (C) 2014 Elsevier Ltd. All rights reserved.</p>10a(R)-(-)-3-Quinuclidinol10aAmberlite IR-12010aBiotransformation10aIon exchange resin10aMucor pinformis1 aKolet, Swati, P.1 aJadhav, Dipesh, D.1 aPriyadarshini, Balaraman1 aSwarge, Bhagyashree, N.1 aThulasiram, Hirekodathakallu, V.  uhttps://library.ncl.res.in/content/fungi-mediated-production-and-practical-purification-r-3-quinuclidinol-002340nas a2200205   4500008004100000022001400041245010400055210006900159260009000228300002600318490000600344520149100350100003101841700002501872700002701897700002201924700001901946700003701965856013202002       2015                        eng d  a2045-232200aFunctional characterization of novel sesquiterpene synthases from Indian sandalwood, santalum album0 aFunctional characterization of novel sesquiterpene synthases fro  aMACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLANDbNATURE PUBLISHING GROUPcMAY  aArticle Number: 100950 v53 a<p>Indian Sandalwood, Santalum album L. is highly valued for its fragrant heartwood oil and is dominated by a blend of sesquiterpenes. Sesquiterpenes are formed through cyclization of farnesyl diphosphate (FPP), catalyzed by metal dependent terpene cyclases. This report describes the cloning and functional characterization of five genes, which encode two sesquisabinene synthases (SaSQS1, SaSQS2), bisabolene synthase (SaBS), santalene synthase (SaSS) and farnesyl diphosphate synthase (SaFDS) using the transcriptome sequencing of S. album. Using Illumina next generation sequencing, 33.32 million high quality raw reads were generated, which were assembled into 84,094 unigenes with an average length of 494.17 bp. Based on the transcriptome sequencing, five sesquiterpene synthases SaFDS, SaSQS1, SaSQS2, SaBS and SaSS involved in the biosynthesis of FPP, sesquisabinene, beta-bisabolene and santalenes, respectively, were cloned and functionally characterized. Novel sesquiterpene synthases (SaSQS1 and SaSQS2) were characterized as isoforms of sesquisabinene synthase with varying kinetic parameters and expression levels. Furthermore, the feasibility of microbial production of sesquisabinene from both the unigenes, SaSQS1 and SaSQS2 in non-optimized bacterial cell for the preparative scale production of sesquisabinene has been demonstrated. These results may pave the way for in vivo production of sandalwood sesquiterpenes in genetically tractable heterologous systems.</p>1 aSrivastava, Prabhakar, Lal1 aDaramwar, Pankaj, P.1 aKrithika, Ramakrishnan1 aPandreka, Avinash1 aShankar, Shiva1 aThulasiram, Hirekodathakallu, V.  uhttps://library.ncl.res.in/content/functional-characterization-novel-sesquiterpene-synthases-indian-sandalwood-santalum-album-102545nas a2200301   4500008004100000022001400041245015600055210006900211260009500280300001200375490000800387520137900395653002301774653003301797653001101830653002101841653002501862100002901887700001601916700002501932700002601957700002401983700002502007700002202032700003702054700002002091856013202111       2016                        eng d  a0006-291X00aFunctional characterization and transient expression manipulation of a new sesquiterpene synthase involved in beta-caryophyllene accumulation in Ocimum0 aFunctional characterization and transient expression manipulatio  a525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USAbACADEMIC PRESS INC ELSEVIER SCIENCEcAPR  a265-2710 v4733 a<p>The genus Ocimum has a unique blend of diverse secondary metabolites, with major proportion of terpenoids including mono- and sesquiterpenes. Although, beta-Caryophyllene, bicyclic sesquiterpene, is one of the major terpene found in Ocimum species and known to possess several biological activities, not much is known about its biosynthesis in Ocimum. Here, we describe isolation and characterization of beta-caryophyllene synthase gene from Ocimum kilimandscharicum Gurke (OkBCS- GenBank accession no. KP226502). The open reading frame of 1629 bp encoded a protein of 542 amino acids with molecular mass of 63.6 kDa and pl value of 5.66. The deduced amino acid sequence revealed 50-70% similarity with known sesquiterpene synthases from angiosperms. Recombinant OkBCS converted farnesyl diphosphate to beta-caryophyllene as a major product (94%) and 6% alpha-humulene. Expression variation of OkBCS well corroborated with beta-caryophyllene levels in different tissues from five Ocimum species. OkBCS transcript revealed higher expression in leaves and flowers. Further, agro-infiltration based transient expression manipulation with OkBCS over-expression and silencing confirmed its role in beta-caryophyllene biosynthesis. These findings may potentially be further utilized to improve plant defense against insect pests. (C) 2016 Elsevier Inc. All rights reserved.</p>10abeta-Caryophyllene10aNatural product biosynthesis10aOcimum10aTerpene synthase10aTransient expression1 aJayaramaiah, Ramesha, H.1 aAnand, Atul1 aBeedkar, Supriya, D.1 aDholakia, Shushan, B.1 aPunekar, Sachin, A.1 aKalunke, Raviraj, M.1 aGade, Wasudeo, N.1 aThulasiram, Hirekodathakallu, V.1 aGiri, Ashok, P.  uhttps://library.ncl.res.in/content/functional-characterization-and-transient-expression-manipulation-new-sesquiterpene-synthase01187nas a2200169   4500008004100000245010300041210006900144260000800213300001400221490000700235520054100242100002300783700002300806700002100829700003700850856013000887       2016                        eng d00aFungal mediated kinetic resolution of racemic acetates to (R)-alcohols using fusarium proliferatum0 aFungal mediated kinetic resolution of racemic acetates to Ralcoh  cOCT  a4563-45670 v573 a<p>Fungal mediated kinetic resolution of seven acyclic/aromatic acetates was achieved using Fusarium proliferatum to furnish (R)-alcohols in high enantiomeric excess (&gt;95%). The kinetic resolution was established as one-pot two-step de-esterification/oxidation biocatalytic process. Further, the preparative scale synthesis of (R)-(+)-1-phenylethanol was accomplished through de-esterification/oxidation of (+/-)-1-phenylethyl acetate using the whole cell of F. proliferatum NCIM 1105. (C) 2016 Elsevier Ltd. All rights reserved.</p>1 aJadhav, Dipesh, D.1 aPatil, Harshal, S.1 aChaya, Patil, S.1 aThulasiram, Hirekodathakallu, V.  uhttps://library.ncl.res.in/content/fungal-mediated-kinetic-resolution-racemic-acetates-r-alcohols-using-fusarium-proliferatum