04223nas a2200277 4500008004100000022001400041245011700055210006900172260000800241300000700249490000700256520327800263653002603541653002303567653002603590653002103616653001303637100002103650700002303671700002203694700002603716700002403742700002403766700002803790856012703818 2023 eng d a1868-695800aGreen adeptness in synthesis of non-toxic copper and cobalt oxide nanocomposites with multifaceted bioactivities0 aGreen adeptness in synthesis of nontoxic copper and cobalt oxide cDEC a790 v143 a

Background: In the present era, we are facing different health problems mainly concerning with drug resistance in microorganisms as well as in cancer cells. In addition, we are also facing the problems of controlling oxidative stress and insect originated diseases like dengue, malaria, chikungunya, etc. originated from mosquitoes. In this investigation, we unfurled the potential of Achatina fulica mucus in green synthesis of mucus mediated copper oxide bio-nanocomposites (SM-CuONC) and cobalt oxide bio-nanocomposites (SM-Co3O4NC). Herein we carried out the physico-chemical characterization like UV-Vis spectra, X-ray diffraction (XRD), Field Emission Scanning Electron Microscopy (FESEM), Transmission electron microscopy (TEM), Energy Dispersive X-ray Analysis (EDAX) and X-ray photoelectron spectroscopy (XPS) of as synthesized bio-nanocomposites. Both the bio-nanocomposites were tested for their potential as antimicrobial activity using well diffusion assay, anticancer activity by MTT assay, antioxidant activity by phosphomolybdenum assay and mosquito larvicidal activity.Results: The results of this study revealed that, SM-CuONC and SM-Co3O4NC were synthesized successfully using A. fulica mucus. The FESEM and TEM data reveal the formation of nanoparticles with quasi-spherical morphology and average particle size of similar to 18 nm for both nanocomposites. The EDAX peak confirms the presence of elemental copper and cobalt in the analyzed samples. The X-ray diffraction analysis confirmed the crystalline nature of the CuO and Co3O4. The result of anti microbial study exhibited that, SM-CuONC showed maximum antimicrobial activity against Escherichia coli NCIM 2065 and Aspergillus fumigatus NCIM 902 which were noted as 2.36 +/- 0.31 and 2.36 +/- 0.59 cm resp. at 60 mu g/well concentration. The result of anticancer activity for SM-CuONC was exhibited as, 68.66 +/- 3.72, 62.66 +/- 3.61 and 71.00 +/- 2.36 percent kill, while SM-Co3O4NC exhibited 61.00 +/- 3.57, 72.66 +/- 4.50 and 71.66 +/- 4.22 percent kill against Human colon cancer (HCT-15), Cervical cancer (HeLa), and Breast cancer (MDA-MB-231) cell lines, respectively, at 20 mu g/well concentration. Both the nanocomposites also exhibited better antioxidant activity. Total antioxidant activity for SM-CuONC at 50 mu g/ml concentration was found to be highest as 55.33 +/- 3.72 while that of SM-Co(3)O(4)Ns was 52.00 +/- 3.22 mM of ascorbic acid/mu g respectively. Both bio-nanocomposites also exhibited 100% mosquito larvicidal activity at concentration ranging from 40 to 50 mg/l. During cytotoxicity study it is noted that at 5 mu g/well concentration, SM-CuO and SM-Co3O4NCs suspension showed more than 97% viability of normal (L929) cell lines. We also studied phytotoxicity of both bio-nanocomposites on Triticum aestivum. In this study, 100% seed germination was observed when seeds are treated with SM-CuONC and SM-Co3O4NC at 500 mg/l and 250 mg/l concentration respectively.Conclusions: This study concludes that in future as synthesized SM-CuONC and SM-Co3O4NC can be used in pharmaceutical, health care system for betterment and welfare of human life as both bio-nanocomposites exhibits better antimicrobial, anticancer, antioxidant and mosquito larvicidal potential.

10aAchatina fulica mucus10aBio-nanocomposites10aBiological activities10aCharacterization10aToxicity1 aMane, Pramod, C.1 aKadam, Deepali, D.1 aKhadse, Ashok, N.1 aChaudhari, Aditya, R.1 aUghade, Supriya, P.1 aAgawane, Sachin, B.1 aChaudhari, Ravindra, D. uhttps://library.ncl.res.in/content/green-adeptness-synthesis-non-toxic-copper-and-cobalt-oxide-nanocomposites-multifaceted04239nas a2200217 4500008004100000245013900041210006900180260000800249300001600257490000600273520343500279100001503714700001803729700002203747700002303769700002603792700002403818700002803842700002403870856012703894 2024 eng d00aGreen synthesis of silver and gold ultra nanocomposites from silk fibroin and their application for treatment of endodontic infections0 aGreen synthesis of silver and gold ultra nanocomposites from sil cJUN a2645–26600 v73 a

The present study is designed to investigate the antibacterial efficacy of B. mori silk fibroin based silver and gold ultra bio-nanocomposites (SF-AgUNC and SF-AuUNC) against Enterococcus faecalis for endodontic disinfection. The SF-AgUNC and SF-AuUNC based irrigant solution was tested in- vitro against Enterococcus faecalis (ATCC: 29212). In this direction, SF-AgUNC and SF-AuUNC synthesized by silk fibroin are very important as they are economically cheap and easy to biosynthesize. The synthesis of SF-AgUNC and SF-AuUNC was accomplished by dissolving 1 mM silver nitrate and 0.5 mM auric chloride respectively in 10 ml of fibroin as precursors. The structural and morphological analysis was executed by UV-Vis Spectroscopy, X-ray diffraction (XRD), Fourier transform infrared spectroscopic analysis (FTIR), Field Emission Scanning Electron Microscopy (FESEM), Transmission electron microscopy (TEM), Energy-dispersive X-ray spectroscopy (EDAX) and X-ray photoelectron spectroscopy (XPS). The qualitative antimicrobial activity of SF-AgUNC and SF-AuUNC were performed against inoculum of Enterococcus faecalis containing 1⨯105 CFU/ ml. The same solutions were tested on dentine specimens inoculated with bacterial cultures and found remarkable biofilm reduction. The structural and morphological analysis reveals the formation of SF-AgUNC and SF-AuUNC having quasi-spherical morphology. The average particle size for SF-AgUNC and SF-AuUNC was same (~ 8 nm). The XRD study confirms that, both SF-AgUNC and SF-AuUNC were crystalline in nature. The bacterial growth inhibition and significant biofilm reduction was observed on dentine specimens treated with SF-AgUNC, SF-AuUNC and positive control. In antibacterial study, at 30 μg/well concentration of SF-AgUNC and SF-AuUNC, 3.13 ± 0.22 and 1.13 ± 0.22 cm of zone of inhibition was noted respectively. Moreover, growth of Enterococcus faecalis was completely inhibited at 20 μg/ml and 30 μg/ml when treated with SF-AgUNC and SF-AuUNC respectively. Hence, the present study concludes that SF-AgUNC and SF-AuUNC can be used as an effective intracanal medicament for the treatment of dental problems with no cyto toxicity.

1 aC., Pramod1 aPawar, Jayant1 aMane, Deepali, P.1 aKhadase, Ashok, N.1 aChaudhari, Aditya, R.1 aUghade, Supriya, P.1 aChaudhari, Ravindra, D.1 aAgawane, Sachin, B. uhttps://library.ncl.res.in/content/green-synthesis-silver-and-gold-ultra-nanocomposites-silk-fibroin-and-their-application04007nas a2200205 4500008004100000245016400041210006900205260000800274300002300282490000700305520319500312100003303507700002403540700001903564700001803583700002503601700002503626700002403651856012603675 2025 eng d00aGreen synthesis and characterization of gold nanoparticles using pomegranate peel extract for inhibition of calcium oxalate crystals and uropathogenic bacteria0 aGreen synthesis and characterization of gold nanoparticles using cAUG aarticle number 5000 v153 a

This study explores the therapeutic potential of pomegranate peel extract (PPE) and its gold nanoparticle conjugate (PPE-AuNP) in the prevention and treatment of urolithiasis. Pomegranate peels collected from four agro-climatic zones of India exhibited consistent methanolic extract yields (10–17%) and total phenolic content, with the highest levels observed in the West zone. Green synthesis of PPE-AuNPs was confirmed through UV–Vis spectroscopy (λmax = 522 nm), DLS (38.2 ± 2.1 nm), zeta potential (-33.66 ± 2.97 mV), and TEM imaging, which showed uniform spherical nanoparticles. Characterization confirmed that PPE-AuNPs were crystalline and phytochemically capped. FTIR shifts in O–H, C = O, and C–O bands, along with a ~ 500 cm-1 Au–O/N vibration, confirmed phytochemical-mediated reduction and stabilization. XRD revealed an FCC structure with a dominant (111) plane, indicating high crystallinity. The synthesized nanoparticles demonstrated antioxidant activity with an IC50 of 21.53 µg/mL, superior to PPE and AuNP alone. In vitro calcium oxalate crystallization assays revealed significant crystal inhibition by PPE-AuNPs, confirmed via E-SEM, EDAX, and XRD analyses. Antibacterial studies against E. coliK. pneumoniaeS. aureus, and uropathogenic E. coli showed MIC values of 31.25–62.5 µg/mL for PPE and PPE-AuNPs, with negligible activity from AuNP alone. In vivo studies in ethylene glycol-induced urolithiatic rats revealed significant improvement in urinary parameters, renal biochemistry (creatinine, urea, SGPT, SGOT), hematological markers, and histopathology, especially in the PPE-AuNP-treated group. PPE-AuNP treatment normalized urinary appearance, reduced serum creatinine and blood urea nitrogen (BUN), and restored renal architecture with minimal degeneration. The enhanced therapeutic effects of PPE-AuNPs are attributed to their bioactive phenolic surface ligands and colloidal stability. Overall, this study demonstrates the synergistic efficacy of PPE-AuNPs as a potent, green nanotherapeutic agent for urolithiasis, offering antioxidant, antibacterial, and nephroprotective effects with promising translational relevance.

1 aMundhewadikar, Dhananjay, M.1 aBhalerao, Minal, R.1 aVairale, Shiva1 aSabir, Safiya1 aMote, Chandrashekhar1 aChowdhury, Chiranjit1 aAgawane, Sachin, B. uhttps://library.ncl.res.in/content/green-synthesis-and-characterization-gold-nanoparticles-using-pomegranate-peel-extract