02444nas a2200217   4500008004100000022001400041245005500055210005500110260006100165300001200226490000700238520177700245653001402022653001102036653001302047653002602060653001602086100001602102700001902118856008902137       2007                        eng d  a1054-547600aMicropropagation of mature pongamia pinnata pierre0 aMicropropagation of mature pongamia pinnata pierre  a233 SPRING STREET, NEW YORK, NY 10013 USAbSPRINGERcDEC  a608-6130 v433 a<p>Murashige and Skoog's (MS) basal medium with benzylaminopurine (BA), kinetin (KN), zeatin (Z), and thidiazuron (TDZ) were tested for induction of multiple shoots from mature-tree-derived axillary meristems of Pongamia pinnata. Sprouting of buds was 64% on medium devoid of plant growth regulators (PGR). Incorporation of BA, KN, or Z was ineffective in enhancing sprouting frequency or induction of multiple shoots. Sprouting was completely suppressed in the presence of TDZ. Caulogenic buds appeared in nodal meristems of these explants after withdrawal of TDZ. The number of shoot buds was more on explants precultured in higher concentrations. At higher concentrations of this PGR, a swelling developed at the axil. Multiple shoot primordia appeared and differentiated from this swelling after culturing these explants on MS medium for six passages of 2 wk each. Shoots were harvested and cultured on 0.45 mu M TDZ for further proliferation. Primary explants after harvesting of shoots were identified as `stump'. Reculturing of stumps on 0.45 mu M TDZ produced more shoots. This step was followed for six cycles to obtain additional shoots in each cycle. Shoots maintained on 0.45 mu M TDZ elongated and rooted (70%) on growth regulator-free medium. Rooted shoots (65%) survived transfer to a sand/soil mixture. This report describes the protocol for micropropagation of P. pinnata using mature-tree-derived nodal meristems. Recycling of mature stock to produce a stream of useable shoots for subculturing and eventual stabilization is of great value and can possibly be generalized as an isolation protocol especially for woody species. Repeated proliferation of caulogenic buds from the same origin may also find application in rescue of endangered germplasm.</p>10aBiodiesel10aPongam10aPongamia10ashoot differentiation10athidiazuron1 aSujatha, K.1 aHazra, Sulekha  uhttps://library.ncl.res.in/content/micropropagation-mature-pongamia-pinnata-pierre-002211nas a2200205   4500008004100000022001400041245008700055210006900142260009200211300001000303490000800313520144000321653001701761653002101778653002901799653001601828100002501844700001901869856011701888       2012                        eng d  a1126-350400aMicropropagation of semecarpus anacardium L.: a medicinally important tree species0 aMicropropagation of semecarpus anacardium L a medicinally import  a4 PARK SQUARE, MILTON PARK, ABINGDON OX14 4RN, OXON, ENGLANDbTAYLOR & FRANCIS LTDcOCT  a61-680 v1463 a<p>Semecarpus anacardium L., a deciduous forest tree, is a source of medicinal compounds. Poor seed-viability restricts the conventional propagation. Micropropagation protocol is standardized for this species. Shoot culture-derived nodal explants were cultured in woody plant media supplemented with thidiazuron (TDZ). Shoot differentiation from meristem was limited. Meristems swelled to form meristematic mass in higher concentrations of TDZ. Swelling of meristem was attributed to the proliferation of meristematic cells. Development of shoots from meristematic mass on withdrawal of TDZ in culture medium indicated the inhibitory influence of TDZ on differentiation of buds to form shoots. Harvesting the primary shoot, leads to appearance of additional shoot buds which elongated on repeated transfer of explants in a medium devoid of growth regulator every four weeks. Optimum (17) number of shoots obtained from each meristem in explants pre-cultured in TDZ 2.27 mu M and re-cultured in growth regulator free medium for seven cycles (28 weeks). This confirms the stimulatory influence of TDZ on proliferation of meristem and inhibitory influence on shoot differentiation. All shoots, rooted in the medium with Indole butyric acid 2.46 mu M. Plantlets survived on transfer to sand: soil (1: 1) mixture and acclimatized. This is the first report on micropropagation of S. anacardium from seedling derived nodal buds using TDZ.</p>10aMeristematic10amicropropagation10aSemecarpus anacardium L.10athidiazuron1 aPanda, Bhuban, Mohan1 aHazra, Sulekha  uhttps://library.ncl.res.in/content/micropropagation-semecarpus-anacardium-l-medicinally-important-tree-species-0