01743nas a2200241   4500008004100000022001400041245008400055210006900139260007300208300000800281490000800289520094500297653000901242653001701251653001801268653002601286653001401312653000801326100001301334700001901347700002101366856011401387       2013                        eng d  a0167-577X00aPhytosynthesis of silver nanoparticles by semecarpus anacardium L. leaf extract0 aPhytosynthesis of silver nanoparticles by semecarpus anacardium   aPO BOX 211, 1000 AE AMSTERDAM, NETHERLANDSbELSEVIER SCIENCE BVcJUL  a5-70 v1023 a<p>Green synthesis of silver nanoparticles (AgNPs) using Semecmpus anacardium L leaf extract was studied. The reduction of silver (Ag+) ions was characterized by using UV-vis spectrophotometer showing formation of AgNPs within 15-20 mm. A time dependent reaction showed the increase in the nanoparticles (NPs) with time. Transmission electron microscopy (TEM) analysis showed that the synthesized AgNPs varied from 10 to 25 nm and has spherical shape. The Fourier transform infrared (FTIR) analysis showed that phenols and protein were responsible for the formation of the AgNPs. The energy dispersive spectroscopy (EDAX) analysis confirms the formed NPs were of silver. The quantification of AgNPs was studied by inductive coupled plasma spectrometry (ICP-AES). The important outcome of this work can be value addition to the medicinal plants in synthesis of NPs for biomedical applications. (C) 2013 Elsevier B.V. All rights reserved.</p>10aFTIR10aLeaf extract10aNanoparticles10aSemecarpus anacardium10asynthesis10aTEM1 aRaju, D.1 aHazra, Sulekha1 aMehta, Urmil, J.  uhttps://library.ncl.res.in/content/phytosynthesis-silver-nanoparticles-semecarpus-anacardium-l-leaf-extract-002416nas a2200229   4500008004100000022001400041245010200055210006900157260000900226300000700235490000600242520164400248653002301892653002101915653001401936653002601950653001901976100002501995700002102020700001902041856012602060       2017                        eng d  a2190-572X00aOptimizing culture conditions for establishment of hairy root culture of semecarpus anacardium L.0 aOptimizing culture conditions for establishment of hairy root cu  cAPR   a210 v73 a<p>Semecarpus anacardium L. is a tree species which produces secondary metabolites of medicinal importance. Roots of the plant have been traditionally used in folk medicines. Different strains of Agrobacterium rhizogenes (A4, ATCC15834 and LBA 9402) were used for induction of hairy roots in in vitro grown tissues of the plant. Hairy root initiation was observed after 25-30 days of infection. Optimum transformation frequency of 61% was achieved on leaf explants with ATCC15834 strain. Infection time of 30 min resulted in greater transformation frequency compared to 10 and 20 min, respectively. The hairy roots cultured in growth regulator-free semi-solid woody plant medium differentiated into callus. Whole shoots infected with ATCC 15834 were found to produce more transformants upon co-cultivation for 4 (65%) and 5 (67%) days. Induction of hairy roots in stem explants infected with ATCC 15834 was lower (52%) compared to leaves (62%) after 4 days of co-cultivation. In A4 and LBA9402 strains transformation efficiency was 49 +/- 2.8% and 36 +/- 5.7% in shoots after 4 days of co-cultivation. Transformation frequency was higher in ATCC15834 strain, irrespective of explants. The hairy roots of S. anacardium elongated slowly upon transfer to half-strength liquid medium. After 3-4 passages in liquid medium slender hairy roots started differentiating which were separated from the original explants. Visible growth of the roots was observed in hormone-free liquid medium after 2-3 months of culturing. Polymerase chain reaction with gene-specific primers from rol A, B and C genes confirms the positive transformation events.</p>10aHairy root culture10aIn vitro culture10arol genes10aSemecarpus anacardium10aTransformation1 aPanda, Bhuban, Mohan1 aMehta, Urmil, J.1 aHazra, Sulekha  uhttps://library.ncl.res.in/content/optimizing-culture-conditions-establishment-hairy-root-culture-semecarpus-anacardium-l