01700nas a2200157   4500008004100000245010000041210006900141260000800210300001600218490000700234520111600241100002101357700001901378700002401397856012101421       2017                        eng d00aDry molten globule-like intermediate during the base-induced unfolding of a multidomain protein0 aDry molten globulelike intermediate during the baseinduced unfol  cNOV  a30207-302160 v193 aThe nature of the initial structural events during the base-induced unfolding of the native (N) state of proteins is poorly understood. Combining site-specific fluorescence resonance energy transfer, size exclusion chromatography, dynamic fluorescence quenching, red-edge excitation shift and circular dichroism spectroscopy, we show here that an early intermediate during the base-induced unfolding of a multidomain protein, i.e., the B form, has features of a dry molten globule. We show that the N (sic) B transition involves protein expansion and loosening of packing of inter-domain helices near domains I and II without the disruption of intra-domain packing or any change in hydration of the inter-domain region which resembles a molten hydrocarbon. Surprisingly, the disruption of inter-domain packing accounts for 40-45% of the total change in free energy of complete unfolding. Our results show that the disruption of van der Waals packing can be decoupled in different regions of a protein and could occur prior to hydrophobic solvation during base-induced unfolding, challenging the existing notion.1 aAcharya, Nirbhik1 aMishra, Prajna1 aJha, Santosh, Kumar  uhttps://library.ncl.res.in/content/dry-molten-globule-intermediate-during-base-induced-unfolding-multidomain-protein00607nas a2200145   4500008004100000245012500041210006900166260000800235300001600243490000700259100002100266700001900287700002400306856013100330       2019                        eng d00aDry molten globule-like intermediate during the base-induced unfolding of a multidomain protein (vol 19, pg 20307, 2017)0 aDry molten globulelike intermediate during the baseinduced unfol  cSEP  a20499-204990 v211 aAcharya, Nirbhik1 aMishra, Prajna1 aJha, Santosh, Kumar  uhttps://library.ncl.res.in/content/dry-molten-globule-intermediate-during-base-induced-unfolding-multidomain-protein-vol-19-pg