02552nas a2200289   4500008004100000022001400041245018000055210006900235260007400304300001000378490000800388520141500396653002101811653002801832653004001860653002101900653003201921653002401953653003801977100001802015700001902033700002002052700001702072700002102089700002602110856012602136       2013                        eng d  a0003-607200aBiochemical correlate of dimorphism in a zygomycete benjaminiella poitrasii: characterization of purified NAD-dependent glutamate dehydrogenase, a target for antifungal agents0 aBiochemical correlate of dimorphism in a zygomycete benjaminiell  aVAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDSbSPRINGERcJUL  a25-360 v1043 a<p>The fungal organisms, especially pathogens, change their vegetative (Y, unicellular yeast and H, hypha) morphology reversibly for survival and proliferation in the host environment. NAD-dependent glutamate dehydrogenase (NAD-GDH, EC 1.4.1.2) from a non-pathogenic dimorphic zygomycete Benjaminiella poitrasii was previously reported to be an important biochemical correlate of the transition process. The enzyme was purified to homogeneity and characterized. It is a 371 kDa native molecular weight protein made up of four identical subunits. Kinetic studies showed that unlike other NAD-GDHs, it may act as an anabolic enzyme and has more affinity towards 2-oxoglutarate than l-glutamate. Chemical modifications revealed the involvement of single histidine and lysine residues in the catalytic activity of the enzyme. The phosphorylation and dephosphorylation study showed that the NAD-GDH is present in active phosphorylated form in hyphal cells of B. poitrasii. Two of the 1,2,3 triazole linked beta-lactam-bile acid conjugates synthesized in the laboratory (B18, B20) were found to be potent inhibitors of purified NAD-GDH which also significantly affected Y-H transition in B. poitrasii. Furthermore, the compound B20 inhibited germ tube formation during Y-H transition in Candida albicans strains and Yarrowia lipolytica. The possible use of NAD-GDH as a target for antifungal agents is discussed.</p>10aAntifungal agent10aBenjaminiella poitrasii10aBiochemical correlate of dimorphism10aCandida albicans10aNAD-GDH activity regulation10aYarrowia lipolytica10aYeast-hypha reversible transition1 aJoshi, C., V.1 aPathan, E., K.1 aPunekar, N., S.1 aTupe, S., G.1 aKapadnis, B., P.1 aDeshpande, Mukund, V.  uhttps://library.ncl.res.in/content/biochemical-correlate-dimorphism-zygomycete-benjaminiella-poitrasii-characterization-001532nas a2200145   4500008004100000245005600041210005400097260001300151300001200164520106400176100001901240700001801259700002201277856008701299       2019                        eng d00aBioprospecting of fungal entomo- and myco-pathogens0 aBioprospecting of fungal entomo and mycopathogens  bSpringer  a497-5133 aThe pest and pathogen control in the field using fungi and their metabolites has indeed gone beyond ‘proof of concept’. However, in view of the performances of these biocontrol fungi in the fields, moreover, acceptability by the end users regarding cost-effectiveness, shelf life, intellectual property rights (IPR), the additional roles, and possible applications are being explored. Dual pathogenicity, viz. entomopathogenesis and mycoparasitism, has added the advantage of wide-spectrum biocontrol in single-crop system. In addition to plant protection, these fungi can promote plant growth. Further, in integrated pest management, the bioremediation of pesticide residues, if any, can also be achieved by these organisms. For value addition, use of cuticle-degrading and mycolytic enzymes produced as killing components can be explored in food, detergent, organic synthesis, and pharmaceutical industries. While exploring the additional applications, the safety to beneficial insects and fungi, biodiversity, and humans, in general, is also addressed.1 aPathan, E., K.1 aPatil, A., V.1 aDeshpande, M., V.  uhttps://library.ncl.res.in/content/bioprospecting-fungal-entomo-and-myco-pathogens