02294nas a2200253   4500008004100000022001400041245011800055210006900173260005700242300001400299490000700313520138200320653001301702653001501715653002401730653002301754100002601777700000901803700001801812700002701830700002401857700002701881856013201908       2013                        eng d  a0735-964000aFunctional characterization of a glucosyltransferase specific to flavonoid 7-O-glucosides from withania somnifera0 aFunctional characterization of a glucosyltransferase specific to  a233 SPRING ST, NEW YORK, NY 10013 USAbSPRINGERcOCT  a1100-11080 v313 a<p>Flavonoids are a large class of phenylpropanoid-derived secondary metabolites, which are usually glycosylated by UDP-glycosyltransferases with one or more sugar groups. Here, we report the cloning and biochemical characterization of a flavonoid glycosyltransferase gene from Withania somnifera (WsGT), which is an important medicinal plant used in Ayurvedic formulations. Using PCR primers, designed for a highly conserved region of previously reported glycosyltransferases, we were able to isolate the corresponding fragment of the WsGT gene. Rapid amplification of cDNA ends (RACE) was then employed to isolate full-length cDNA, which had an open reading frame of 1,371 bp that encode for 456 amino acids. Phylogenetic analysis indicated that WsGT was similar to that of family 1 GT-B glycosyltransferase. Biochemical analysis revealed that WsGT interacts with UDP-glucose and was capable of regiospecifically glycosylating flavonoid-7-ols, such as apigenin, naringenin, luteolin, diadzein and genistein. Expression profiling studies showed that WsGT was highly expressed in young and mature leaves of W. somnifera. Furthermore, exposure to salicylic acid enhanced the expression of WsGT in the leaves and heat shock treatment resulted in decreased expression of WsGT after an initial increase. This may suggest the role of WsGT in response to abiotic/biotic stresses.</p>10aDiadzein10aFlavonoids10aGlycosyltransferase10aWithania somnifera1 aKumar, R., J. Santosh1 aRuby1 aSingh, Somesh1 aSonawane, Prashant, D.1 aVishwakarma, R., K.1 aKhan, Bashir, Mohammad  uhttps://library.ncl.res.in/content/functional-characterization-glucosyltransferase-specific-flavonoid-7-o-glucosides-withania-001726nas a2200169   4500008004100000020002000041022002000061245011200081210006900193260002500262300001200287520107300299100002401372700001501396700001701411856012801428       2016                        eng d  a978-981101085-9  a978-981101084-200aMemory booster plant Bacopa monniera (brahmi): biotechnology and molecular aspects of bacoside biosynthesis0 aMemory booster plant Bacopa monniera brahmi biotechnology and mo  aSingaporebSpringer   a167-1893 aBacopa monniera (Brahmi) has been used in the Ayurvedic system of medicine as a brain tonic, memory enhancer, antianxiety, cardiotonic, anticancer, anti-inflammatory, analgesic, and anticonvulsant agent since ancient times. These pharmacological properties are mainly attributed to the triterpenoid saponins present in the extracts of the plant. Biosynthesis of triterpenoid saponins starts from the isoprenoid pathway through farnesyl pyrophosphate by cyclization of 2,3-oxidosqualene, resulting in the formation of triterpenoid backbones. The plant produces relatively smaller amounts of bacosides, and to overcome this shortage, a large amount of biomass is used in the pharmaceutical preparations. Despite a wealth of medicinal importance, the molecular characterization and pathway engineering of bacoside biosynthesis in Bacopa remain unexplored. In this chapter, we have briefly discussed the research findings on in vitro plant regeneration, genetic transformation, and molecular characterization of some of the genes involved in the biosynthesis of bacosides.1 aVishwakarma, R., K.1 aKumari, U.1 aKhan, B., M.  uhttps://library.ncl.res.in/content/memory-booster-plant-bacopa-monniera-brahmi-biotechnology-and-molecular-aspects-bacoside