01828nas a2200217   4500008004100000022001400041245015700055210006900212260009900281300001200380490000800392520089100400653003201291653002601323653003501349653003201384100002001416700002101436700002601457856012701483       2013                        eng d  a0960-852400aSupplementation of medium with diammonium hydrogen phosphate enhanced the D-lactate dehydrogenase levels leading to increased D-lactic acid productivity0 aSupplementation of medium with diammonium hydrogen phosphate enh  aTHE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLANDbELSEVIER SCI LTDcOCT  a736-7390 v1463 a<p>The production of D-lactic acid by Lactobacillus lactis RM2-24 was investigated using modified media to increase the efficiency of the fermentation process. The results indicated that the addition of 5 g/l peptone and 1 g/l (NH4)(2)HPO4 enhanced D-lactic acid production by 32%, as compared to that obtained from non supplemented media, with a productivity of 3.0 g/l/h. Lactate dehydrogenase (LDH) expression profile in these different media was studied which resulted in appearance of additional LDH isoform produced by cells when they were grown in HSYE supplemented with (NH4)(2)HPO4. The additional LDH appears to be L-LDH contributing to production of L-lactic acid in the fermented broth. This is totally new information in the lactic acid fermentation and could be very useful to industries engaged in D-lactic acid production. (c) 2013 Elsevier Ltd. All rights reserved.</p>10aD(-) lactic acid production10aLactate dehydrogenase10aLactobacillus delbrueckii Uc-310aLactobacillus lactis RM2-241 aSinghvi, Mamata1 aJadhav, Akanksha1 aGokhale, Digambar, V.  uhttps://library.ncl.res.in/content/supplementation-medium-diammonium-hydrogen-phosphate-enhanced-d-lactate-dehydrogenase-0