01895nas a2200265 4500008004100000022001400041245008600055210006900141260013700210300001400347490000700361520091500368653002701283653002701310653001701337653000801354100001801362700002201380700001601402700001501418700002901433700002201462700002501484856012001509 2011 eng d a0956-566300aQuantum dot based immunosensor using 3D circular microchannels fabricated in PDMS0 aQuantum dot based immunosensor using 3D circular microchannels f aOXFORD FULFILLMENT CENTRE THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLANDbELSEVIER ADVANCED TECHNOLOGYcFEB a3050-30530 v263 a

Microchannel is basic functional component of microfluidic chip and every step-forward of its construction technique has been receiving concern all over the world. The present work describes a novel, rapid and simple fabrication technique for building 3D microchannels in poly(dimethyl siloxane) (PDMS) elastomer. These microchannels were used for rapid detection of antigens (E. coli) by quantum dot (QD) based approach. Luminescent QD (CdTe) were synthesized by aqueous method and characterized using high resolution transmission electron microscopy (HRTEM), fluorescence spectroscopy and X-ray diffraction (XRD). The QDs were functionalized with anti-E. coli antibodies for immuno-detection. The reported process allowed easier and faster method of fabrication of circular 3D micochannels and demonstrated their potential use in an immuno-biosensor device. (C) 2010 Elsevier B.V. All rights reserved.

10aCapillary driven flows10aCircular microfluidics10aImmunosensor10aQDs1 aMorarka, Amit1 aAgrawal, Shailaja1 aKale, Sonia1 aKale, Anup1 aOgale, Satishchandra, B.1 aPaknikar, Kishore1 aBodas, Dhananjay, S. uhttps://library.ncl.res.in/content/quantum-dot-based-immunosensor-using-3d-circular-microchannels-fabricated-pdms-002658nas a2200313 4500008004100000022001400041245010900055210006900164260007400233300002600307490000700333520156000340653001801900653001501918653001901933653002201952653001801974653002801992100002202020700002102042700002202063700002002085700001802105700002302123700001702146700002202163700002902185856013002214 2013 eng d a1388-076400aHollow nanogold/meso-magnetite composite: pulsed laser synthesis, properties, and biosensing application0 aHollow nanogoldmesomagnetite composite pulsed laser synthesis pr aVAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDSbSPRINGERcNOV aArticle No. UNSP 20810 v153 a

A facile and template-free UV (Excimer) laser photolysis process is demonstrated to transform a Fe-complex into unique hollow Fe3O4 nanosphere morphology with each sphere having nanometric pores and an opening. Depending on the Fe-complex concentration and processing time interesting configurations are seen to evolve. When an identical process is applied to a mixture of Fe-complex and chloroauric acid an enthrallingly decorated Au-Fe3O4 nanostructure evolves, with Au nanoparticles surface-loaded on mesospheric Fe3O4. This room temperature process implemented under normal laboratory conditions is clearly versatile and applicable to heterojunction nanomaterials synthesis in a single-step process. The potential application of these gold-decorated magnetic nanostructures was also investigated for immuno-magnetic capture of E. coli in biosensing and these were found to be sensitive even below 1,000 cfu/ml. The test results demonstrate linear sensing response in the range of 10(3)-10(5) cfu/ml. We also show that these nanostructures can be used for simple electrical conductivity-based biosensing since they show dramatic conductivity change in a simple drop-cast test. A new laser-based approach to the synthesis of unique hollow sphere morphology of magnetite (Fe3O4) without and with Au nanoparticle decoration is presented. The potential application of these gold-decorated magnetic nanostructures was also investigated for immuno-magnetic capture of E. coli in biosensing and these were found to be sensitive even below 1,000 cfu/ml.

10aE. coli cells10aFe-complex10aMeso-magnetite10aNanobiotechnology10aNanocomposite10aPulse laser irradiation1 aChaudhari, Nilima1 aWarule, Sambhaji1 aAgrawal, Shailaja1 aThakare, Vishal1 aJouen, Samuel1 aHannoyer, Beatrice1 aKale, Bharat1 aPaknikar, Kishore1 aOgale, Satishchandra, B. uhttps://library.ncl.res.in/content/hollow-nanogoldmeso-magnetite-composite-pulsed-laser-synthesis-properties-and-biosensing-002563nas a2200265 4500008004100000022001400041245013200055210006900187260000900256300001000265490000700275520167700282653001801959653001501977653002201992653002702014653001302041100002202054700002102076700001602097700002402113700001702137700002202154856012102176 2015 eng d a0920-506300aFluorescent cadmium telluride quantum dots embedded chitosan nanoparticles: a stable, biocompatible preparation for bio-imaging0 aFluorescent cadmium telluride quantum dots embedded chitosan nan cJAN  a42-560 v263 a

Fluorescent cadmium telluride quantum dots (CdTe QDs) are an optically attractive option for bioimaging, but are known to display high cytotoxicity. Nanoparticles synthesized from chitosan, a natural biopolymer of beta 1-4 linked glucosamine, display good biocompatibility and cellular uptake. A facile, green synthetic strategy has been developed to embed green fluorescent cadmium telluride quantum dots (CdTe QDs) in biocompatible CNPs to obtain a safer preparation than `as is' QDs. High-resolution transmission electron microscopy showed the crystal lattice corresponding to CdTe QDs embedded in CNPs while thermogravimetry confirmed their polymeric composition. Electrostatic interactions between thiol-capped QDs (4nm, -57mV) and CNPs (\~300nm, +38mV) generated CdTe QDs-embedded CNPs that were stable up to three months. Further, viability of NIH3T3 mouse fibroblast cells in vitro increased in presence of QDs-embedded CNPs as compared to bare QDs. At the highest concentration (10 mu g/ml), the former shows 34 and 39% increase in viability at 24 and 48h, respectively, as compared to the latter. This shows that chitosan nanoparticles do not release the QDs up to 48h and do not cause extended toxicity. Furthermore, hydrolytic enzymes such as lysozyme and chitinase did not degrade chitosan nanoparticles. Moreover, QDs-embedded CNPs show enhanced internalization in NIH3T3 cells as compared to bare QDs. This method offers ease of synthesis and handling of stable, luminescent, biocompatible CdTe QDs-embedded CNPs with a favorable toxicity profile and better cellular uptake with potential for bioimaging and targeted detection of cellular components.

10aBiocompatible10abioimaging10aCdTe quantum dots10achitosan nanoparticles10aToxicity1 aGhormade, Vandana1 aGholap, Haribhau1 aKale, Sonia1 aKulkarni, Vaishnavi1 aBhat, Suresh1 aPaknikar, Kishore uhttps://library.ncl.res.in/content/fluorescent-cadmium-telluride-quantum-dots-embedded-chitosan-nanoparticles-stable02212nas a2200181 4500008004100000245013800041210006900179260000800248300001200256490000800268520152300276100002801799700002101827700001801848700002201866700002201888856012001910 2016 eng d00aRadio-frequency triggered heating and drug release using doxorubicin-loaded LSMO nanoparticles for bimodal treatment of breast cancer0 aRadiofrequency triggered heating and drug release using doxorubi cSEP a878-8900 v1453 aRadio-frequency responsive nanomaterials combined with drugs for simultaneous hyperthermia and drug delivery are potential anti-cancer agents. In this study, chitosan coated La0.7Sr0.3MnO3 nanoparticles (C-LSMO NPs) were synthesized and characterized by X-ray diffraction, dynamic light scattering, Fourier transform infra red spectroscopy, vibrating sample magnetometer, scanning electron and atomic force microscopy, Under low radio-frequency (365 kHz, RF), C-LSMO NPs (90 nm) showed good colloidal stability (+22 mV), superparamagnetic nature (15.4 emu/g) and heating capacity (57.4W/g SAR value). Chitosan facilitated doxorubicin entrapment (76%) resulted in DC-LSMO NPs that showed drug release upon a 5 min RF exposure. MCF-7 and MDA-MB-231 cancer cells responded to a 5 min RF exposure in the presence of bimodal DC-LSMO NPs with a significant decrease in viability to 73% and 88% (Pearson correlation, r = 1, P<0.01) respectively, as compared to hyperthermia alone. Internalization of DC-LSMO NPs via the endosomal pathway led to an efficient localization of doxorubicin within the cell nucleus. The ensuing DNA damage, heat shock protein induction, and caspase production triggered apoptotic cell death. Moreover, DC-LSMO NPs successfully restricted the migration of metastatic MDA-MB-231 cancer cells. These data suggest that DC-LSMO NPs are potential bimodal therapeutic agents for cancer treatment and hold promise against disease recurrence and drug resistance. (C) 2016 Elsevier B.V. All rights reserved.1 aKulkarni, Vaishnavi, M.1 aBodas, Dhananjay1 aDhoble, Deepa1 aGhormade, Vandana1 aPaknikar, Kishore uhttps://library.ncl.res.in/content/radio-frequency-triggered-heating-and-drug-release-using-doxorubicin-loaded-lsmo04670nas a2200205 4500008004100000245011000041210006900151260000800220300001400228490000700242520391400249100002104163700002204184700002404206700002304230700003404253700002704287700002204314856012804336 2019 eng d00a Development of a nano-gold immunodiagnostic assay for rapid on-site detection of invasive aspergillosis 0 aDevelopment of a nanogold immunodiagnostic assay for rapid onsit cSEP a1341-13520 v683 a

Introduction. Timely detection of invasive aspergillosis (IA) caused by fungal pathogens, i.e. Aspergillus fumigatus and Aspergillus flavus, in immunocompromised patients is crucial in preventing high mortality.

Aim. To develop a simple immunoassay for the detection of galactomannan (GM), an IA biomarker.

Methodology. GM from A. fumigatus and A. flavus clinical strains was purified and characterized by X-ray diffraction, IR spectroscopy and C-13/H-1 nuclear magnetic resonance (NMR) for polyclonal antibody (pAb) production in rabbits. An enzyme-linked immunosorbent assay (ELISA) was standardized using concanavalin A to capture Aspergillus GM and pAbs to detect it. Gold nanoparticles (AuNPs) were synthesized and conjugated to pAbs for the development of a dot-blot immunoassay. The developed dot-blot was evaluated with 109 clinical serum and bronchoalveolar lavage samples.

Results. Spectroscopy studies characterized the D-galactofuranosyl groups of GM responsible for the immune response and generation of pAbs. The ELISA employing pAbs showed a sensitivity of 1 ng ml(-1) for Aspergillus GM. Furthermore, a sensitive, visual, rapid dot-blot assay developed by the conjugation of pAbs to AuNPs (similar to 24 +/- 5 nm size, -36 +/- 2 mV zeta potential) had a detection limit of 1 pg ml(-1) in serum. The pAbs interacted with Aspergillus spp. but did not cross-react with other fungal pathogen genera such as Penicillium and Candida. Evaluation of the dot-blot with 109 clinical samples showed high sensitivity (80%) and specificity (93.2 %), with an overall assay accuracy of 89%.

Conclusion. The developed nano-gold immunodiagnostic assay has immense potential for practical use in rapid, specific and sensitive on-site diagnosis of IA, even under resource-limited settings.

1 aRaval, Komal, M.1 aGhormade, Vandana1 aRajamohanan, P., R.1 aChoudhary, Hansraj1 aRudramurthy, Shivaprakash, M.1 aChakrabarti, Arunaloke1 aPaknikar, Kishore uhttps://library.ncl.res.in/content/development-nano-gold-immunodiagnostic-assay-rapid-site-detection-invasive-aspergillosis