01875nas a2200241   4500008004100000022001400041245013700055210006900192260007300261300001200334490000700346520092200353653002801275653002501303653002201328653003101350653002401381100002301405700002001428700002301448700003001471856013201501       2009                        eng d  a0927-776500aIn situ synthesis of water dispersible bovine serum albumin capped gold and silver nanoparticles and their cytocompatibility studies0 aIn situ synthesis of water dispersible bovine serum albumin capp  aPO BOX 211, 1000 AE AMSTERDAM, NETHERLANDSbELSEVIER SCIENCE BVcOCT  a224-2280 v733 a<p>A simple and convenient one step room temperature method is described for the synthesis of bovine serum albumin (BSA) capped gold and silver nanoparticles. BSA reduces silver ions to silver nanoparticles but does not directly reduce gold ions to gold nanoparticles at room temperature and varying pH conditions. However, when silver and gold ions are simultaneously added to BSA, silver ions get reduced to metallic silver first and these in turn reduce gold ions to gold nanoparticles through a galvanic exchange reaction. The so synthesized silver and gold nanoparticles are easily water dispersible and can withstand addition of salt even at high concentrations. It is shown that the capped protein retains its secondary structure and the helicity to a large extent on the nanoparticles surface and that the protein capping makes the nanoparticles cytocompatible. (C) 2009 Elsevier B.V. All rights reserved.</p>10aAg and Au nanoparticles10aBovine serum albumin10aCytocompatibility10aGalvanic exchange reaction10aReductive synthesis1 aMurawala, Priyanka1 aPhadnis, S., M.1 aBhonde, Ramesh, R.1 aPrasad, Bhagavatula L. V.  uhttps://library.ncl.res.in/content/situ-synthesis-water-dispersible-bovine-serum-albumin-capped-gold-and-silver-nanoparticles-001545nas a2200193   4500008004100000022001400041245011300055210006900168260011000237300001200347490000600359520075400365100001801119700002301137700001501160700001801175700003001193856012801223       2012                        eng d  a2040-336400aGellan gum capped silver nanoparticle dispersions and hydrogels: cytotoxicity and in vitro diffusion studies0 aGellan gum capped silver nanoparticle dispersions and hydrogels   aTHOMAS GRAHAM HOUSE, SCIENCE PARK, MILTON RD, CAMBRIDGE CB4 0WF, CAMBS, ENGLANDbROYAL SOC CHEMISTRYcOCT  a563-5670 v43 a<p>The preparation of highly stable water dispersions of silver nanoparticles using the naturally available gellan gum as a reducing and capping agent is reported. Further, exploiting the gel formation characteristic of gellan gum silver nanoparticle incorporated gels have also been prepared. The optical properties, morphology, zeta potential and long-term stability of the synthesized silver nanoparticles were investigated. The superior stability of the gellan gum-silver nanoparticle dispersions against pH variation and electrolyte addition is revealed. Finally, we studied the cytotoxicity of AgNP dispersions in mouse embryonic fibroblast cells (NIH3T3) and also evaluated the in vitro diffusion of AgNP dispersions/gels across rat skin.</p>1 aDhar, Sheetal1 aMurawala, Priyanka1 aShiras, A.1 aPokharkar, V.1 aPrasad, Bhagavatula L. V.  uhttps://library.ncl.res.in/content/gellan-gum-capped-silver-nanoparticle-dispersions-and-hydrogels-cytotoxicity-and-vitro-002002nas a2200253   4500008004100000022001400041245013400055210006900189260007300258300001200331490000700343520106300350653001401413653002501427653001901452653001701471653002301488653002301511100002301534700002001557700001901577700003001596856012201626       2014                        eng d  a0928-493100aIn situ synthesized BSA capped gold nanoparticles: effective carrier of anticancer drug methotrexate to MCF-7 breast cancer cells0 aIn situ synthesized BSA capped gold nanoparticles effective carr  aPO BOX 211, 1000 AE AMSTERDAM, NETHERLANDSbELSEVIER SCIENCE BVcJAN  a158-1670 v343 a<p>The proficiency of MTX loaded BSA capped gold nanoparticles (Au-BSA-MTX) in inhibiting the proliferation of breast cancer cells MCF-7 as compared to the free drug Methotrexate (MTX) is demonstrated based on MTT and Ki-67 proliferation assays. In addition, DNA ladder gel electrophoresis studies, flow cytometry and TUNEL assay confirmed the induction of apoptosis by MTX and Au-BSA-MTX in MCF-7 cells. Notably, Au-BSA-MTX: was found to have higher cytotoxicity on MCF-7 cells compared with an equivalent dose of free MTX. The enhanced activity is attributed to the preferential uptake of Au-BSA-MTX particles by MCF-7 cells due to the presence of BSA that acts as a source of nutrient and energy to the rapidly proliferating MCF-7 cells. Moreover, the targeting ability of the drug MTX to the over expressed folate receptors on MCF-7 cells also contributes to the enhanced uptake and activity. Taken together, these results unveil that Au-BSA-MTX could be more effective than free drug for cancer treatment. (C) 2013 Elsevier B.V. All rights reserved.</p>10aApoptosis10aBovine serum albumin10aCancer therapy10aCytotoxicity10aDual active agents10agold nanoparticles1 aMurawala, Priyanka1 aTirmale, Amruta1 aShiras, Anjali1 aPrasad, Bhagavatula L. V.  uhttps://library.ncl.res.in/content/situ-synthesized-bsa-capped-gold-nanoparticles-effective-carrier-anticancer-drug-0