01275nas a2200253   4500008004100000022001400041245006000055210006000115260009500175300001200270490000800282520047900290653001200769653001200781653001700793653001800810653001600828653001100844100001900855700001800874700002100892700001900913856008900932       2011                        eng d  a0003-269700aMethod to extract intact and pure RNA from mycobacteria0 aMethod to extract intact and pure RNA from mycobacteria  a525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USAbACADEMIC PRESS INC ELSEVIER SCIENCEcOCT  a286-2880 v4173 a<p>We describe a high-yielding, simple, and aerosol-free protocol for the isolation of RNA from mycobacteria that does not require sophisticated instruments. The method yielded 50 mu g of RNA from 10(7) cells, 50 times more than a recently reported method. Our method can extract total RNA from aerobically grown bacteria and from in vitro hypoxia-induced dormant bacilli and mycobacteria residing within infected macrophages. (C) 2011 Elsevier Inc. All rights reserved.</p>10aDormant10aHypoxia10aMycobacteria10aRNA isolation10aSpheroplast10aTrizol1 aAkhtar, Shamim1 aSarkar, Sampa1 aMishra, Abhishek1 aSarkar, Dhiman  uhttps://library.ncl.res.in/content/method-extract-intact-and-pure-rna-mycobacteria-001185nas a2200157   4500008004100000245010300041210006900144260005100213520053400264100001700798700001900815700001900834700001700853700002100870856013600891       2015                        eng d00aMechanistic understanding of rapid gelation of silk fibroin using a biosurfactant – sophorolipid0 aMechanistic understanding of rapid gelation of silk fibroin usin  aNational Chemical Laboratory, Pune  IndiacFEB3 a<p>3D polymeric hydrogels find use in biomedical applications as scaffolds and drug delivery vehicle • Silk fibroin a structural protein is a promising candidate (excellent thermo-mechanicals, biocompatibility, biodegradability) • Sophorolipid (SL)-a biosurfactant is known to exhibit antimicrobial, anti cancerous and cell differentiating. • Use of SL as gelling agent can enhance the applicability of SF hydrogels • SF-SL scaffolds obtained by lyophilization of hydrogels can find application in tissue regeneration</p>1 aDubey, Parul1 aNawale, Laxman1 aSarkar, Dhiman1 aNisal, Anuya1 aPrabhune, Asmita  uhttps://library.ncl.res.in/content/mechanistic-understanding-rapid-gelation-silk-fibroin-using-biosurfactant-%E2%80%93-sophorolipid01427nas a2200133   4500008004100000245010100041210006900142260000800211490000600219520089900225100001801124700001901142856013201161       2015                        eng d00aMultifunctional silica nanoparticles for pancreatic cancer specific drug delivery and bioimaging0 aMultifunctional silica nanoparticles for pancreatic cancer speci  cJUN0 v23 a<p>Mesoporous silica nanoparticles (MSN) have attracted increasing interest as drug carriers due to their biocompatibility and robustness. In this analysis we explored the potential of MSN as nanotheranostic platform for pancreatic cancer specific drug delivery and bioimaging. Pancreatic cancer is the most fatal cancer and due to its high mortality and low prognosis it is imperative to develop new methods for early diagnosis and therapy of this cancer. Though traditionally organic dyes, quantum dots and fluorescent agents are used for bioimaging but here we have utilized graphene quantum dots (GQDs) for bioimaging due to their superior biocompatibility and fluorescence properties. MSN were loaded with gemcitabine; the most preferred drug for pancreatic cancer. MSN and GQDs were characterized by SEM, TEM, FT-IR etc and the efficacy of nanoformulation was determined by MTT assay.</p>1 aNigam, Preeti1 aSarkar, Dhiman  uhttps://library.ncl.res.in/content/multifunctional-silica-nanoparticles-pancreatic-cancer-specific-drug-delivery-and-bioimaging02700nas a2200349   4500008004100000022001400041245011800055210006900173260000800242490000700250520161600257653000901873653001201882653001801894653002601912653001101938653001801949653001901967653001801986653002202004653002402026653001502050653001202065653002702077100002502104700002002129700002002149700002002169700001902189700001902208856012302227       2017                        eng d  a0928-493100aMultifunctional inulin tethered silver-graphene quantum dots nanotheranostic module for pancreatic cancer therapy0 aMultifunctional inulin tethered silvergraphene quantum dots nano  cSEP0 v783 a<p><span style="color: rgb(51, 51, 51); font-family: arial, helvetica, sans-serif; font-size: 13px; background-color: rgb(248, 248, 248);">Cancer nanotechnology is an emerging area of cancer diagnosis and therapy. Although considerable progress has been made for targeted drug delivery systems to deliver anticancer agents to particular site of interest, new nanomaterials are frequently being developed and explored for better drug delivery efficiency. In the present work, we have explored a novel nanoformulation based on silver-graphene quantum dots (Ag-GQDs) nanocomposite for its successful implementation for pancreatic cancer specific drug delivery in wistar rats. Carboxymethyl inulin (CMI); a modified variant of natural polysaccharide inulin is tethered with the nanocomposite via carbodiimide coupling to enhance the biocompatibility of nanoformulation. Experiments are performed to investigate the cytotoxicity reduction of silver nanoparticles after inulin tethering as well as anticancer efficacy of the system using 5-Fluorouracil (5-FU) as model drug. SEM, TEM, FT-IR, UV-vis, photoluminescence and anti proliferative assays (MTT) are performed for characterisation of the nanocomposite. Hyaluronic acid (HA) is conjugated as targeting moiety for CD-44 (cancer stem cell marker) to fabricate a complete targeted drug delivery vehicle specific for pancreatic cancer. In the present work two prime objectives were achieved; mitigation the toxicity of silver nanoparticles by inulin coating and it's in vivo application for pancreatic cancer. (C) 2017 Elsevier B.V. All rights reserved.</span></p>10aacid10aDextran10aDrug-delivery10agraphene quantum dots10aInulin10aNanocomposite10aNanocomposites10aNanoparticles10aPancreatic Cancer10aSilver nanoparticle10aStrategies10aSystems10aTargeted Drug Delivery1 aJoshi, Preeti, Nigam1 aAgawane, Sachin1 aAgawane, Sachin1 aAgawane, Sachin1 aSarkar, Dhiman1 aSarkar, Dhiman  uhttps://library.ncl.res.in/content/multifunctional-inulin-tethered-silver-graphene-quantum-dots-nanotheranostic-module02321nas a2200193   4500008004100000022001400041245013500055210006900190260000800259300001600267490000600283520160500289100002301894700002501917700001701942700002001959700001901979856012901998       2018                        eng d  a2046-206900aMolecular modeling studies and in vitro screening of dihydrorugosaflavonoid and its derivatives against mycobacterium tuberculosis0 aMolecular modeling studies and in vitro screening of dihydrorugo  cMAY  a10634-106430 v83 a<p>Novel drug regimens against tuberculosis (TB) are urgently needed and may be developed by targeting essential enzymes of Mtb that sustain the pathogenicity of tuberculosis. In the present investigation, series of compounds (5a-f and 6a-f) based on a naturally occurring rugosaflavonoid moiety were evaluated by in silico molecular modeling studies against -ketoacyl-ACP reductase (MabA) (PDB ID: IUZN) and pantothenate kinase (PanK) (PDB ID: ; 3AF3). Compounds 5a, 5c, 5d, and 6c, which had docking scores of -8.29, -8.36, -8.17 and -7.39 kcal mol(-1), respectively, displayed interactions with MabA that were better than those of isoniazid (-6.81 kcal mol(-1)). Similarly, compounds 5a, 5c, 5d, and 6c, which had docking scores of -7.55, -7.64, -7.40 and -6.7 kcal mol(-1), respectively, displayed interactions with PanK that were comparable to those of isoniazid (-7.64 kcal mol(-1)). Because of their docking scores, these compounds were screened in vitro against Mycobacterium tuberculosis H37Ra (Mtb) using an XRMA protocol. Among the screened compounds, the dihydrorugosaflavonoid derivatives 5a, 5c, and 5d had IC50 values of 12.93, 8.43 and 11.3 g mL(-1), respectively, and exhibited better inhibitory activity than the parent rugosaflavonoid derivatives. The rugosaflavonoid derivative 6c had an IC50 value of 17.57 g mL(-1). The synthesized compounds also displayed inhibitory activity against the Gram-positive bacteria Bacillus subtilis and Staphylococcus aureus. The present study will be helpful for the further development of these molecules into antitubercular lead candidates.</p>1 aPuranik, Ninad, V.1 aSrivastava, Pratibha1 aSwami, Sagar1 aChoudhari, Amit1 aSarkar, Dhiman  uhttps://library.ncl.res.in/content/molecular-modeling-studies-and-vitro-screening-dihydrorugosaflavonoid-and-its-derivatives