03206nas a2200193 4500008004100000245011200041210006900153260000800222300001200230490000700242520252000249100001902769700002002788700001702808700002102825700001702846700002002863856012902883 2019 eng d00aEffect of media components and growth conditions for improved linoleic acid production by beauveria species0 aEffect of media components and growth conditions for improved li cAUG a945-9540 v963 a

Beauveria species are well-known insect pathogenic fungi, and Beauveria bassiana is used as a biopesticide against various pests in agriculture. However, the Beauveria species has not been reported as producers of microbial oils. In this study, Beauveria spp. MTCC 5184 was used to produce microbial oil with high linoleic acid (LA) content. Ten experiments were performed to evaluate the effects of several media parameters, such as carbon and nitrogen sources, pH, various concentrations of carbon and nitrogen, growth duration, and oleic acid (OLA) supplementation for maximum LA and dry biomass production by the fungus. Several of these parameters had a significant impact on the production of LA, as well as dry biomass. The glucose yeast extract (GYE) medium supplemented with 1.5% (w/v) peptone yielded maximum LA (0.32 +/- 0.01 g L-1) and biomass (5.51 +/- 0.26 g L-1). However, through the addition of 1.0% (w/v) OLA, the precursor of LA, LA production was enhanced 12-fold (1.24 +/- 0.03 g L-1), and the biomass production increased by 5-fold (11.05 +/- 0.46 g L-1) in comparison to those in the basal (GYE) medium. Using lactose as the sole carbon source produced the lowest LA (0.05 +/- 0.00 g L-1) and biomass (1.04 +/- 0.10 g L-1). The results of this study will be useful for the commercial exploitation of this fungus for the production of LA-rich microbial oil for use in the production of lubricants, greases, paints, cosmetics, etc.

1 aSanghi, Smrati1 aChirmade, Tejas1 aMore, Snehal1 aPrabhune, Asmita1 aGupta, Vidya1 aKadoo, Narendra uhttps://library.ncl.res.in/content/effect-media-components-and-growth-conditions-improved-linoleic-acid-production-beauveria02344nas a2200289 4500008004100000022001400041245008000055210006900135260000900204300001100213490000800224520144400232653002501676653003301701653001501734653001201749100002301761700002201784700002101806700001901827700002101846700002101867700001701888700002201905700002101927856010601948 2021 eng d a0959-652600aEnergy and life cycle impact assessment for xylitol production from corncob0 aEnergy and life cycle impact assessment for xylitol production f cJAN  a1232170 v2783 a

The conversion of biobased feedstocks into energy and value-added chemicals is one main approach to address the current global challenge for waste mitigation through the biorefinery mode. Corncob biomass which is one of the most abundant agricultural residue in India with a high hemicellulose content, can serve as a potential low-cost raw material for food-grade xylitol production. The present study demonstrates a process for xylitol production from corncob biomass with detailed energy and life cycle analysis for viability assessment. The major highlight of the process is the use of microbial fermentation, where biomass-derived D-xylose is converted to xylitol with high selectivity. Overall, 0.502 kg of xylitol crystals could be produced from 3.5 kg of corncob biomass. Simulation analysis revealed that the evaporators are the primary consumers of energy, and the process of heat integration can significantly reduce the energy requirements of the overall process. Environmental impacts of the system evaluated showed emission results of 8.68 kg CO2 equivalent and revealed that marine aquatic and freshwater eco-toxicity are the only possible contributors to the environment. The results suggest that the process would have favourable energy balances, which can be used in pilot plant and heat exchanger network design and operation for xylitol production scale up to 500 L. (C) 2020 Elsevier Ltd. All rights reserved.

10abiomass valorization10aLife cycle impact assessment10asimulation10axylitol1 aDasgupta, Diptarka1 aSidana, Arushdeep1 aGhosh, Prasenjit1 aSharma, Tripti1 aSingh, Jasvinder1 aPrabhune, Asmita1 aMore, Snehal1 aBhaskar, Thallada1 aGhosh, Debashish uhttps://library.ncl.res.in/content/energy-and-life-cycle-impact-assessment-xylitol-production-corncob02060nas a2200277 4500008004100000022001400041245010300055210006900158260000800227300000800235490000700243520117200250653001901422653001101441653001101452653002101463653002001484100002401504700002301528700002601551700001701577700001801594700001801612700002401630856012801654 2025 eng d a0959-399300aEnzymatic biodegradation of used engine oil using a novel lipase derived from renewable feedstocks0 aEnzymatic biodegradation of used engine oil using a novel lipase cNOV a4480 v413 a

This study introduces an eco-friendly enzymatic approach for biodegrading used engine oil, utilizing a novel lipase from the mesophilic yeast strain Pichia Caribbica. The yeast was cultured in detoxified corncob hydrolysate with used cooking oil as an inducer. OVAT study confirmed that the optimal pH and temperature for lipase production by the strain was 7.0 and 25 degrees Celsius, respectively, with lipase activity of 70 IU/mL. The enzyme effectively degraded similar to 46% of used engine oil over a period of 15 days. Gas Chromatography-Mass Spectrometry (GC-MS) and FT-IR analysis confirmed a significant reduction in hydrocarbon concentrations and the emergence of new functional groups, confirming the enzyme's ability to degrade complex hydrocarbons into less toxic derivatives. These findings highlight the lipase's potential as an effective biocatalyst for eco-friendly bioremediation of oil-contaminated environments, such as soils and aquatic systems, while also emphasizing its role in environmental management through the reuse of waste by-products in enzyme production, thus reducing the environmental impact of improper waste disposal.

10aBiodegradation10aLipase10aPC570310aUsed cooking oil10aUsed engine oil1 aTracy, Preetanshika1 aDasgupta, Diptarka1 aAshok, Patel, Pratima1 aMore, Snehal1 aSarkar, Bipul1 aPorwal, Jyoti1 aTripathi, Deependra uhttps://library.ncl.res.in/content/enzymatic-biodegradation-used-engine-oil-using-novel-lipase-derived-renewable-feedstocks