02792nas a2200337 4500008004100000022001400041245011000055210006900165260007100234300001200305490000600317520166100323653001301984653002501997653001502022653001502037653002102052100001702073700002002090700002002110700002002130700002202150700002102172700002102193700002202214700001902236700002702255700001702282700002502299856013002324 2014 eng d a2168-048500aUptake of Azo dyes into silk glands for production of colored silk cocoons using a green feeding approach0 aUptake of Azo dyes into silk glands for production of colored si a1155 16TH ST, NW, WASHINGTON, DC 20036 USAbAMER CHEMICAL SOCcFEB a312-3170 v23 a

Dyeing of textile fabrics is considered to be one of the most polluting industries today, and there is a need to develop green processes that can reduce this pollution. A promising technology that can potentially cleanup the dyeing of silk fibers that are widely used for textile applications would involve the generation of intrinsically colored silk cocoons. This can be achieved by feeding of Bombyx mori silkworm larvae with a modified feed of mulberry leaves containing a sprayed dye solution. This process significantly reduces the need for treating toxic dye effluents that are generated in traditional dyeing processes. In this report, we have evaluated a set of seven different azo dyes that are used in the textile industry for dyeing to produce intrinsically dyed silk. The dyes used in the study had similar chemical structures with systematically varying partition coefficients. The results suggest that while some dyes produced intrinsically colored silk other did not. Careful evaluation of the physical properties of these related azo dyes suggest that the balance of hydrophobic and hydrophilic character is necessary for diffusion of the dye from the alimentary canal of the silkworm larva into the hemolymph and later into the silk glands. The partition coefficient of the dye also determines the preferential association of the dye with either sericin or fibroin protein in the silkworm gland and finally into the cocoon. These insights are extremely important in development of novel dye molecules that can be successfully fed to Bombyx mori silkworm larvae for producing intrinsically colored silk of various colors and shades.

10aAzo dyes10aBiochemical pathways10aColor silk10aDye uptake10a`' Green `' silk1 aNisal, Anuya1 aTrivedy, Kanika1 aMohammad, Hasan1 aPanneri, Suyana1 aGupta, Sayam, Sen1 aLele, Ashish, K.1 aManchala, Ramesh1 aKumar, Nirmal, S.1 aGadgil, Mugdha1 aKhandelwal, Harish, B.1 aMore, Snehal1 aLaxman, Ryali, Seeta uhttps://library.ncl.res.in/content/uptake-azo-dyes-silk-glands-production-colored-silk-cocoons-using-green-feeding-approach-003206nas a2200193 4500008004100000245011200041210006900153260000800222300001200230490000700242520252000249100001902769700002002788700001702808700002102825700001702846700002002863856012902883 2019 eng d00aEffect of media components and growth conditions for improved linoleic acid production by beauveria species0 aEffect of media components and growth conditions for improved li cAUG a945-9540 v963 a

Beauveria species are well-known insect pathogenic fungi, and Beauveria bassiana is used as a biopesticide against various pests in agriculture. However, the Beauveria species has not been reported as producers of microbial oils. In this study, Beauveria spp. MTCC 5184 was used to produce microbial oil with high linoleic acid (LA) content. Ten experiments were performed to evaluate the effects of several media parameters, such as carbon and nitrogen sources, pH, various concentrations of carbon and nitrogen, growth duration, and oleic acid (OLA) supplementation for maximum LA and dry biomass production by the fungus. Several of these parameters had a significant impact on the production of LA, as well as dry biomass. The glucose yeast extract (GYE) medium supplemented with 1.5% (w/v) peptone yielded maximum LA (0.32 +/- 0.01 g L-1) and biomass (5.51 +/- 0.26 g L-1). However, through the addition of 1.0% (w/v) OLA, the precursor of LA, LA production was enhanced 12-fold (1.24 +/- 0.03 g L-1), and the biomass production increased by 5-fold (11.05 +/- 0.46 g L-1) in comparison to those in the basal (GYE) medium. Using lactose as the sole carbon source produced the lowest LA (0.05 +/- 0.00 g L-1) and biomass (1.04 +/- 0.10 g L-1). The results of this study will be useful for the commercial exploitation of this fungus for the production of LA-rich microbial oil for use in the production of lubricants, greases, paints, cosmetics, etc.

1 aSanghi, Smrati1 aChirmade, Tejas1 aMore, Snehal1 aPrabhune, Asmita1 aGupta, Vidya1 aKadoo, Narendra uhttps://library.ncl.res.in/content/effect-media-components-and-growth-conditions-improved-linoleic-acid-production-beauveria02344nas a2200289 4500008004100000022001400041245008000055210006900135260000900204300001100213490000800224520144400232653002501676653003301701653001501734653001201749100002301761700002201784700002101806700001901827700002101846700002101867700001701888700002201905700002101927856010601948 2021 eng d a0959-652600aEnergy and life cycle impact assessment for xylitol production from corncob0 aEnergy and life cycle impact assessment for xylitol production f cJAN  a1232170 v2783 a

The conversion of biobased feedstocks into energy and value-added chemicals is one main approach to address the current global challenge for waste mitigation through the biorefinery mode. Corncob biomass which is one of the most abundant agricultural residue in India with a high hemicellulose content, can serve as a potential low-cost raw material for food-grade xylitol production. The present study demonstrates a process for xylitol production from corncob biomass with detailed energy and life cycle analysis for viability assessment. The major highlight of the process is the use of microbial fermentation, where biomass-derived D-xylose is converted to xylitol with high selectivity. Overall, 0.502 kg of xylitol crystals could be produced from 3.5 kg of corncob biomass. Simulation analysis revealed that the evaporators are the primary consumers of energy, and the process of heat integration can significantly reduce the energy requirements of the overall process. Environmental impacts of the system evaluated showed emission results of 8.68 kg CO2 equivalent and revealed that marine aquatic and freshwater eco-toxicity are the only possible contributors to the environment. The results suggest that the process would have favourable energy balances, which can be used in pilot plant and heat exchanger network design and operation for xylitol production scale up to 500 L. (C) 2020 Elsevier Ltd. All rights reserved.

10abiomass valorization10aLife cycle impact assessment10asimulation10axylitol1 aDasgupta, Diptarka1 aSidana, Arushdeep1 aGhosh, Prasenjit1 aSharma, Tripti1 aSingh, Jasvinder1 aPrabhune, Asmita1 aMore, Snehal1 aBhaskar, Thallada1 aGhosh, Debashish uhttps://library.ncl.res.in/content/energy-and-life-cycle-impact-assessment-xylitol-production-corncob02381nas a2200289 4500008004100000022001400041245008400055210006900139260000900208300001100217490000800228520148700236653001201723653001701735653002701752653002501779653001201804100001801816700002301834700002801857700002101885700001701906700001801923700001901941700002201960856010901982 2022 eng d a0926-669000aCrystalline xylitol production from corncob biomass with oral toxicity analysis0 aCrystalline xylitol production from corncob biomass with oral to cNOV  a1154070 v1873 a

Xylitol, a five-carbon sugar alcohol, has a steady global market and finds application as a natural sugar substitute in various food and confectionery products. Biocatalytic xylitol production, although touted as a greener alternative to conventional chemical catalysis, suffers from certain challenges, the primary being high cost of production. This study demonstrates a process for food-grade xylitol production from corncob biomass with energy reduction through two major process modifications. A non-conventional fermentation strategy was adopted whereby adjusting aeration without agitation, xylitol with high yield (0.86 +/- 0.015 g/g), and productivity (0.74 gL(-1)h(-1)) could be produced by a GRAS Pichia caribbica MTCC 5703 strain. Xylitol was recovered from the broth in the form of crystals using a combination of membrane-based filtration and crystallization. The crystals demonstrated similar to 98 % purity when quantified with H-1 NMR. Oral toxicity analysis of the crystals demonstrated no adverse effect in female Winstar rats (at a loading of 2000 mg/kg body weight of animals). Overall process statistics showed that 0.584 kg of food-grade xylitol crystals could be produced from 3.5 kg of corncob biomass. The two-process modifications during fermentation and xylitol recovery enabled an energy saving of similar to 20.842 kW/kg of crystals, providing tremendous advantages for biorefinery-based large-scale xylitol production from corncob biomass.

10aBiomass10aFermentation10aOral toxicity analysis10aWastewater treatment10axylitol1 aAhuja, Vishal1 aDasgupta, Diptarka1 aKshirsagar, Siddheshwar1 aGhosh, Prasenjit1 aMore, Snehal1 aGupta, Piyush1 aBehera, Babita1 aBhaskar, Thallada uhttps://library.ncl.res.in/content/crystalline-xylitol-production-corncob-biomass-oral-toxicity-analysis02115nas a2200289 4500008004100000022001400041245010000055210006900155260000800224300001000232490000800242520122000250653001201470653001201482653001901494653001701513653002201530653001201552100002301564700002201587700001801609700001701627700002101644700002201665700001501687856012301702 2022 eng d a0960-308500aProcess development for crystalline xylitol production from corncob biomass by Pichia caribbica0 aProcess development for crystalline xylitol production from corn cMAY a45-560 v1333 a

Xylitol, a commodity chemical, is widely used in nutraceutical and pharmaceutical formulations. Microbial xylitol production is a promising alternative to mitigate current industrial practice issues and offers an environment-friendly sustainable conversion route. This study demonstrates a bioprocess for xylitol production from corncob using a mesophilic yeast Pichia caribbica. Corncob was hydrolyzed by dilute acid and steam explosion to recover fermentable xylose and used as the feed for xylitol production. Activated carbon treatment (3% w/v) completely removed the biomass-derived inhibitors furfural and hydroxymethyl furfural from the liquid hydrolysate. The fermenting yeast Pichia caribbica produced 124.1 +/- 0.45 g/L xylitol from the detoxified and concentrated corncob hydrolysate with a high yield of 0.80 +/- 0.02 g/g. The crystallized xylitol with 96.5% purity demonstrated no harmful effects on the cell line used as a control for the in-vitro toxicity studies. This proof of concept can be applied to help scale up for bio-refinery-based large-scale production of xylitol from corncob biomass.(c) 2022 Institution of Chemical Engineers. Published by Elsevier Ltd. All rights reserved.

10aBiomass10aCorncob10aDetoxification10aFermentation10aToxicity analysis10axylitol1 aDasgupta, Diptarka1 aSidana, Arushdeep1 aSarkar, Bipul1 aMore, Snehal1 aGhosh, Debashish1 aBhaskar, Thallada1 aRay, Anjan uhttps://library.ncl.res.in/content/process-development-crystalline-xylitol-production-corncob-biomass-pichia-caribbica01602nas a2200277 4500008004100000022001400041245011400055210006900169260000800238300001100246490000700257520070400264653002300968653001900991653001701010653001701027653001201044100001801056700002801074700002101102700001801123700001601141700001701157700002301174856012701197 2022 eng d a2213-292900aProcess development for detoxification of corncob hydrolysate using activated charcoal for xylitol production0 aProcess development for detoxification of corncob hydrolysate us cFEB a1070970 v103 aThe work describes process development for detoxification of corncob hydrolysate using activated carbon for microbial xylitol production. Activated carbon was used as an adsorbent to completely remove the major fermentation inhibitors obtained during dilute acid and steam explosion of biomass. A xylitol yield of 0.78 g/g was achieved from the detoxified hydrolysate in a 5 L fermenter by Pichia caribbica. The spent carbon was effectively regenerated and used for three consecutive cycles of operation. The successive regeneration and reuse of carbon could reduce the operational cost by similar to 38% and be used as a reference dataset for process scale-up and cost-effective xylitol production.10aActivated charcoal10aDetoxification10aFermentation10aRegeneration10axylitol1 aAhuja, Vishal1 aKshirsagar, Siddheshwar1 aGhosh, Prasenjit1 aSarkar, Bipul1 aSutar, Ajit1 aMore, Snehal1 aDasgupta, Diptarka uhttps://library.ncl.res.in/content/process-development-detoxification-corncob-hydrolysate-using-activated-charcoal-xylitol01688nas a2200229 4500008004100000022001400041245011100055210006900166260000800235300001100243490000800254520092400262653001501186653002601201653001101227653001201238653001201250100002401262700002301286700001701309856013201326 2023 eng d a0926-669000aChallenges and opportunities for production of C5 sugar fatty acid esters (SFAEs) from renewable resources0 aChallenges and opportunities for production of C5 sugar fatty ac cMAR a1161700 v1933 a

Sugar fatty acid esters represent a billion-dollar market with huge global demand. Although glucose and sucrose-based surfactants have prevailed in the chemical industry for decades, C-5-based esters are relatively unknown and have recently started to gain attention owing to their multifunctional properties. These molecules can be derived from cheap and inexpensive biomass/renewable resources and present promising potential with diverse applications as additives in various nutraceutical, pharmaceutical, and cosmeceutical formulations. However, large-scale production of C-5 sugar esters is limited, with one or two commercial plants operating worldwide. This review highlights the major challenges and prospects of biotechnological production of the C-5 sugar esters, considering the possible advancements over existing technology with various aspects of industrial bioprocessing and product recovery.

10aBioprocess10aC-5-fatty acid esters10aLipase10asolvent10axylitol1 aTracy, Preetanshika1 aDasgupta, Diptarka1 aMore, Snehal uhttps://library.ncl.res.in/content/challenges-and-opportunities-production-c5-sugar-fatty-acid-esters-sfaes-renewable-resources01751nas a2200265 4500008004100000022001400041245008800055210006900143260000800212300000800220490000700228520093200235653002401167653001201191653001701203653002401220653001201244100002301256700001801279700002001297700001701317700002101334700001701355856011301372 2023 eng d a0959-399300aFood-grade xylitol production from corncob biomass with acute oral toxicity studies0 aFoodgrade xylitol production from corncob biomass with acute ora cAPR a1020 v393 a

Xylitol, a sugar substitute, is widely used in various food formulations and finds a steady global market. In this study, xylitol crystals were produced from corncob by fermentation (as an alternative to the chemical catalytic process) by a GRAS yeast Pichia caribbica MTCC 5703 and characterized in detail for their purity and presence of any possible contaminant that may adversely affect mammalian cell growth and proliferation. The acute and chronic oral toxicity trials demonstrated no gross pathological changes with average weekly weight gain in female Wistar rats at high xylitol loading (LD50 > 10,000 mg/kg body weight). The clinical chemistry analysis supported the evidence of no dose-dependent effect by analyzing blood biochemical parameters. The finding suggests the possible application of the crystals (> 98% purity) as a food-grade ingredient for commercial manufacture pending human trials.

10aAcute oral toxicity10aCorncob10aFermentation10aShelf life analysis10axylitol1 aDasgupta, Diptarka1 aAhuja, Vishal1 aSingh, Raghuvir1 aMore, Snehal1 aMudliar, Sandeep1 aKumar, Madan uhttps://library.ncl.res.in/content/food-grade-xylitol-production-corncob-biomass-acute-oral-toxicity-studies02060nas a2200277 4500008004100000022001400041245010300055210006900158260000800227300000800235490000700243520117200250653001901422653001101441653001101452653002101463653002001484100002401504700002301528700002601551700001701577700001801594700001801612700002401630856012801654 2025 eng d a0959-399300aEnzymatic biodegradation of used engine oil using a novel lipase derived from renewable feedstocks0 aEnzymatic biodegradation of used engine oil using a novel lipase cNOV a4480 v413 a

This study introduces an eco-friendly enzymatic approach for biodegrading used engine oil, utilizing a novel lipase from the mesophilic yeast strain Pichia Caribbica. The yeast was cultured in detoxified corncob hydrolysate with used cooking oil as an inducer. OVAT study confirmed that the optimal pH and temperature for lipase production by the strain was 7.0 and 25 degrees Celsius, respectively, with lipase activity of 70 IU/mL. The enzyme effectively degraded similar to 46% of used engine oil over a period of 15 days. Gas Chromatography-Mass Spectrometry (GC-MS) and FT-IR analysis confirmed a significant reduction in hydrocarbon concentrations and the emergence of new functional groups, confirming the enzyme's ability to degrade complex hydrocarbons into less toxic derivatives. These findings highlight the lipase's potential as an effective biocatalyst for eco-friendly bioremediation of oil-contaminated environments, such as soils and aquatic systems, while also emphasizing its role in environmental management through the reuse of waste by-products in enzyme production, thus reducing the environmental impact of improper waste disposal.

10aBiodegradation10aLipase10aPC570310aUsed cooking oil10aUsed engine oil1 aTracy, Preetanshika1 aDasgupta, Diptarka1 aAshok, Patel, Pratima1 aMore, Snehal1 aSarkar, Bipul1 aPorwal, Jyoti1 aTripathi, Deependra uhttps://library.ncl.res.in/content/enzymatic-biodegradation-used-engine-oil-using-novel-lipase-derived-renewable-feedstocks