02078nas a2200253   4500008004100000022001400041245019000055210006900245260007900314300001200393490000800405520106000413653002801473653002501501653002401526653002101550653002701571100001601598700001801614700001901632700002101651700002001672856013201692       2009                        eng d  a0273-228900aEffect of cultural conditions and media constituents on production of penicillin V acylase and CTAB treatment to enhance whole-cell enzyme activity of rhodotorula aurantiaca (NCIM 3425)0 aEffect of cultural conditions and media constituents on producti  a999 RIVERVIEW DRIVE SUITE 208, TOTOWA, NJ 07512 USAbHUMANA PRESS INCcJUN  a463-4720 v1573 a<p>Penicillin V acylase (PVA) is a pharmaceutically important enzyme as it plays a vital role in the manufacture of semi-synthetic beta-lactam antibiotics. Rhodotorula aurantiaca (NCIM 3425) produced high levels of intracellular penicillin V acylase after 18 h at pH 8.0 and temperature 27 degrees C. Fructose was the best carbon source for PVA production, whereas tryptone was the best nitrogen source to produce the enzyme up to 170 and 1,088 IU/l of culture, respectively. Additionally, the cell-bound PVA activity was enhanced on treatment with cationic detergent. Whole-cell activity was found to be doubled (204%) on treatment of 0.01 g dry weight of cells with 50 mu g/ml solution of N-cetyl-N,N,N-trimethylammoniumbromide at pH 8.0 for 1 h at room temperature. Atomic force microscopy images of permeabilized cells show perturbation in the cell wall and offer first-ever visual illustration of surface structure modifications that occur during permeabilization of R. aurantiaca cells leading to enhancement in activity of intracellular enzyme.</p>10aAtomic force microscopy10aIntracellular enzyme10aPenicillin Vacylase10aPermeabilization10aRhodotorula aurantiaca1 aKumar, Atul1 aSingh, Sanjay1 aPoddar, Pankaj1 aPrabhune, Asmita1 aPundle, Archana  uhttps://library.ncl.res.in/content/effect-cultural-conditions-and-media-constituents-production-penicillin-v-acylase-and-ctab-001925nas a2200229   4500008004100000022001400041245013500055210006900190260007300259300001000332490000700342520105100349653002401400653002101424653001901445653002101464653002501485653002101510100001601531700002001547856012801567       2009                        eng d  a1381-117700aEffect of organic solvents on cell-bound penicillin V acylase activity of erwinia aroideae (DSMZ 30186): a permeabilization effect0 aEffect of organic solvents on cellbound penicillin V acylase act  aPO BOX 211, 1000 AE AMSTERDAM, NETHERLANDSbELSEVIER SCIENCE BVcMAY  a67-710 v573 a<p>Erwinia aroideae (DSMZ 30186) is a potential microbial culture to produce intracellular penicillin V acylase (PVA). The whole cell PVA activity was improved by permeabilization with various organic solvents. The cell-bound PVA activity showed an eightfold increase upon treatment with chloroform (5 mu L/mg(dry) (biomass)) for 10 min and diethyl ether (10 mu L/mg(dry) (biomass)) for 45 min. Hexane, toluene, ethyl acetate and dichloromethane enhanced the enzyme activity up to two-, six-, four- and two-fold, respectively; whereas, PVA activity declined drastically on permeabilization with acetone, pyridine and alcohols. The physicochemical properties of the organic solvents used for permeabilization were correlated with the change in activity. It was found that solvents with high hydrophobicity (log P &gt; 0.68) and lower dielectric constant (&lt; 9) were relatively effective in increasing PVA activity. These results allow systematic selection of suitable solvent for best performance. (c) 2008 Elsevier B.V. All rights reserved.</p>10adielectric constant10aErwinia aroideae10aHydrophobicity10aOrganic solvents10aPenicillin V acylase10aPermeabilization1 aKumar, Atul1 aPundle, Archana  uhttps://library.ncl.res.in/content/effect-organic-solvents-cell-bound-penicillin-v-acylase-activity-erwinia-aroideae-dsmz-0