02623nas a2200217 4500008004100000022001400041245008300055210006900138260010600207300001400313490000700327520181700334653002402151653001802175653002302193653001102216653002202227100002202249700001702271856011702288 2013 eng d a0025-540800aFungus mediated synthesis of biomedically important cerium oxide nanoparticles0 aFungus mediated synthesis of biomedically important cerium oxide aTHE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLANDbPERGAMON-ELSEVIER SCIENCE LTDcOCT a4134-41380 v483 a

Nanomaterials can be synthesized by chemical, physical and the more recently discovered biological routes. The biological routes are advantageous over the chemical and physical ones as unlike these, the biological synthesis protocols occur at ambient conditions, are cheap, non-toxic and eco-friendly. Although purely biological and bioinspired methods for the synthesis of nanomaterials are environmentally benign and energy conserving processes, their true potential has not been explored yet and attempts are being made to extend the formation of technologically important nanoparticles using microorganisms like fungi. Though there have been reports on the biosynthesis of oxide nanoparticles by our group in the past, no attempts have been made to employ fungi for the synthesis of nanoparticles of rare earth metals or lanthanides. Here we report for the first time, the bin-inspired synthesis of biomedically important cerium oxide (CeO2) nanoparticles using the thermophilic fungus Humicola sp. The fungus Humicola sp. when exposed to aqueous solutions of oxide precursor cerium (III) nitrate hexahydrate (CeN3O9 center dot 6H(2)O) results in the extracellular formation of CeO2 nanoparticles containing Ce (III) and Ce (IV) mixed oxidation states, confirmed by X-ray Photoemission Spectroscopy (XPS). The formed nanoparticles are naturally capped by proteins secreted by the fungus and thus do not agglomerate, are highly stable, water dispersible and are highly fluorescent as well. The biosynthesized nanoparticles were characterized by UV-vis spectroscopy, Photoluminescence spectroscopy (PL), Transmission Electron Microscopy (TEM), Fourier Transform Infrared Spectroscopy (FTIR), X-Ray Diffraction (XRD) and X-ray Photoemission Spectroscopy (XPS). (c) 2013 Elsevier Ltd. All rights reserved.

10aElectron microscopy10aNanostuctures10aOptical properties10aOxides10aX-ray diffraction1 aKhan, Shadab, Ali1 aAhmad, Absar uhttps://library.ncl.res.in/content/fungus-mediated-synthesis-biomedically-important-cerium-oxide-nanoparticles-001842nas a2200217 4500008004100000022001400041245008200055210006900137260006900206300001200275490000800287520110600295653001201401653002201413653001301435653001601448653000901464100002201473700001701495856011201512 2013 eng d a1385-894700aPhase, size and shape transformation by fungal biotransformation of bulk TiO20 aPhase size and shape transformation by fungal biotransformation  aPO BOX 564, 1001 LAUSANNE, SWITZERLANDbELSEVIER SCIENCE SAcAUG a367-3710 v2303 a

Nanosynthesis is an ever expanding frontier in recent years in view of its implications to many future technologies covering diverse fields. Considerable progress has been made realizing high quality synthesis of elemental nanomaterials as well as compounds as chemical, biological and physical routes. The issue of technologically viable large scale synthesis still continues to be a challenge. Here we demonstrate a novel environmentally friendly top down approach to nanosynthesis which exploits the strength and peculiarities of fungus based bioleaching in extracting radicals from compounds and then providing them with a reactive as well as capping environment. Thus protein capped nanoparticles of TiO2 (5-28 nm, circular and brookite phase) are formed directly from micron size (150-250 nm, disc shape, anatase phase) powder by exposing them to a medium of fungus Humicola sp. at just 50 degrees C. The fungus Humicola sp. is not only useful for the synthesis of nanoparticles but also for the transformation of shape, phase and size of TiO2. (C) 2013 Elsevier B.V. All rights reserved.

10aAnatase10aBiotransformation10aBrookite10ahumicola sp10aTiO21 aKhan, Shadab, Ali1 aAhmad, Absar uhttps://library.ncl.res.in/content/phase-size-and-shape-transformation-fungal-biotransformation-bulk-tio2-001889nas a2200157 4500008004100000022001400041245015900055210006900214260011000283300001400393490000600407520115000413100002201563700001701585856012901602 2014 eng d a2046-206900aEnzyme mediated synthesis of water-dispersible, naturally protein capped, monodispersed gold nanoparticles; their characterization and mechanistic aspects0 aEnzyme mediated synthesis of waterdispersible naturally protein  aTHOMAS GRAHAM HOUSE, SCIENCE PARK, MILTON RD, CAMBRIDGE CB4 0WF, CAMBS, ENGLANDbROYAL SOC CHEMISTRYcJUL a7729-77340 v43 a

Inorganic nanomaterials are conventionally synthesized under harsh environments like extremes of temperature, pressure and pH. These methods are eco-unfriendly, expensive, toxic, cumbersome, yield bigger particles which agglomerate due to not being capped by capping agents. In contrast, biological synthesis of inorganic nanomaterials occurs under ambient conditions viz. room temperature, atmospheric pressure, physiological pH and is reliable, eco-friendly and cheap. We have already reported the extracellular biosynthesis of monodispersed gold nanoparticles from the whole cells of novel extremophilic actinomycete Thermomonospora sp. In order to know the exact mechanism of synthesis, we decided to investigating it further. Here we describe the simple protocol for purification of the temperature and SDS resistant sulfite reductase enzyme and organic capping molecule, which are required for the synthesis and stabilization of gold nanoparticles respectively. This purified enzyme was then employed for the synthesis of gold nanoparticles along with the capping molecule, which render gold nanoparticles monodispersed in solution.

1 aKhan, Shadab, Ali1 aAhmad, Absar uhttps://library.ncl.res.in/content/enzyme-mediated-synthesis-water-dispersible-naturally-protein-capped-monodispersed-gold-001826nas a2200229 4500008004100000022001400041245016600055210006900221260008600290300001200376490000600388520089900394653001901293653002001312653002101332653001601353653003701369100002201406700002001428700001701448856013101465 2014 eng d a2190-428600aExtracellular biosynthesis of gadolinium oxide (Gd2O3) nanoparticles, their biodistribution and bioconjugation with the chemically modified anticancer drug taxol0 aExtracellular biosynthesis of gadolinium oxide Gd2O3 nanoparticl aTRAKEHNER STRASSE 7-9, FRANKFURT AM MAIN, 60487, GERMANYbBEILSTEIN-INSTITUTcMAR a249-2570 v53 a

As a part of our programme to develop nanobioconjugates for the treatment of cancer, we first synthesized extracellular, protein-capped, highly stable and well-dispersed gadolinium oxide (Gd2O3) nanoparticles by using thermophilic fungus Humicola sp. The biodistribution of the nanoparticles in rats was checked by radiolabelling with Tc-99m. Finally, these nanoparticles were bioconjugated with the chemically modified anticancer drug taxol with the aim of characterizing the role of this bioconjugate in the treatment of cancer. The biosynthesized Gd2O3 nanoparticles were characterized by UV-vis spectroscopy, transmission electron microscopy (TEM), X-ray diffraction (XRD) and X-ray photoemission spectroscopy (XPS). The Gd2O3-taxol bioconjugate was confirmed by UV-vis spectroscopy and fluorescence microscopy and was purified by using high performance liquid chromatography (HPLC).

10abioconjugation10abiodistribution10agadolinium oxide10ahumicola sp10atransmission electron microscopy1 aKhan, Shadab, Ali1 aGambhir, Sanjay1 aAhmad, Absar uhttps://library.ncl.res.in/content/extracellular-biosynthesis-gadolinium-oxide-gd2o3-nanoparticles-their-biodistribution-and-001490nas a2200181 4500008004100000022001400041245018700055210006900242260000900311300001200320490000600332520077400338100002201112700001701134700001601151700001701167856012401184 2014 eng d a1932-620300aFungus-mediated preferential bioleaching of waste material such as fly - ash as a means of producing extracellular, protein capped, fluorescent and water soluble silica nanoparticles0 aFungusmediated preferential bioleaching of waste material such a cSEP  ae1075970 v93 a

In this paper, we for the first time show the ability of the mesophilic fungus Fusarium oxysporum in the bioleaching of waste material such as Fly-ash for the extracellular production of highly crystalline and highly stable, protein capped, fluorescent and water soluble silica nanoparticles at ambient conditions. When the fungus Fusarium oxysporum is exposed to Fly-ash, it is capable of selectively leaching out silica nanoparticles of quasi-spherical morphology within 24 h of reaction. These silica nanoparticles have been completely characterized by UV-vis spectroscopy, Photoluminescence (PL), Transmission electron microscopy (TEM), X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR) and Energy dispersive analysis of Xrays (EDAX).

1 aKhan, Shadab, Ali1 aUddin, Imran1 aMoeez, Sana1 aAhmad, Absar uhttps://library.ncl.res.in/content/fungus-mediated-preferential-bioleaching-waste-material-such-fly-ash-means-producing01574nas a2200265 4500008004100000022001400041245011300055210006900168260000900237300001000246490000700256520067800263653002800941653001300969653001900982653001401001653001301015653002401028100001601052700002201068700003101090700002701121700003001148856013001178 2020 eng d a1751-825300aOvalbumin-mediated synthesis and simultaneous functionalization of graphene with increased protein stability0 aOvalbuminmediated synthesis and simultaneous functionalization o cJAN  a60-670 v133 a

In this article, we have shown an easy, one-step, protein-directed approach for the synthesis of water-soluble and functional graphene using ovalbumin. The obtained ovalbumin functionalized graphene was characterized by UV-visible spectroscopy, X-ray diffraction, Raman spectroscopy, and Fourier transform infrared spectroscopy. Atomic force microscopy was used to check the attachment/functionalization of ovalbumin on grapheme sheets. It was shown that the functionalization of ovalbumin on grapheme sheets makes the protein more thermally stable compare to free ovalbumin, which has been shown by using sodium dodecyl sulfate polyacrylamide gel electrophoresis.

10aAtomic force microscopy10agraphene10agraphene oxide10aOvalbumin10aSDS-PAGE10athermal degradation1 aUllah, Riaz1 aKhan, Shadab, Ali1 aAladresi, Aref, Ali Mohamm1 aAlharbi, Sulaiman, Ali1 aChinnathambi, Arunachalam uhttps://library.ncl.res.in/content/ovalbumin-mediated-synthesis-and-simultaneous-functionalization-graphene-increased-protein